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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: divineverdance] * 1
    #29288980 -

B Traven said:
LadysKnight said:
Theoretically speaking, if you had a clean, fully colonized jar of grain spawn, do you think it would be dangerous to open the jar, sniff contents, replace lid until spawned a couple weeks later?


Pssshhhh...

I've routinely swapped lids, transferred spawn to old carryout containers for storage because I need the jar and don't have space for the spawn anywhere else, and even just dumped spawn into a tub and then let it ait there because I got busy. (That last one I don't do anymore because I finally figured out that it's way easier to mix spawn in mixing bowl and then just dump it into the tub all pre-mixed.) I've then let that shit sit for so long that I had to pick pins off the surface before spawning.

It's always the EXCESSIVE time till finally spawning that fucks me up. I'm trying to break those habits and tighten up my scheduling, because the complacency doesn't do me any favors and eventually stuff dries out or gets funky. But exposing colonized grain and then letting it sit a few weeks? Nah. I don't lose a second of sleep over it. Opening for sniff tests is a pretty major part of my process lol


you are diabolical. takeout containers?  haha.  unreal dude.

divineverdance said:
how long after birthing do you see fruits, usually/


hes talking about spawn.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: misterswisher] * 2
    #29288981 -

misterswisher said:
Yeah using food dehydrator the fruits were dried properly. Stored improperly.

Idk if anyone has a bat signal for Nichrome but I really need his expertise right now or anyone who knows really 😂
WHAT THE FUCK IS THIS? I’m more excited than worried about it. Is it a slime mold? If it is I’m definitely keeping it as a pet.



Tubs riddled with a pine smell. It’s like 4th or 5th flush contams expected lmao.



Looks filimentous, so more likely some iffy mold. There are a lot of yellow molds, everything from penicillium to fusarium can be yellow like that.  That school bus yellow looks like xanthone.


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Question everything, it's very responsive.

Culture Storage

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29289070 -

Is it normal that panaeolus bisporus mycelium changes the dye this much? I was worried about contams at first but after taking a good look it seems like the mycelium just ate the dye/ changed the hue which I've seen before, just not this aggressively. I've got the same goin on my ochra plates.


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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv] * 1
    #29289080 -

SkanskKorv said:
Is it normal that panaeolus bisporus mycelium changes the dye this much? I was worried about contams at first but after taking a good look it seems like the mycelium just ate the dye/ changed the hue which I've seen before, just not this aggressively. I've got the same goin on my ochra plates.



What % of DME are you using? If it is low and there is anything useful(it usually is) in the food color then the mycelium will digest it.


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29289083 -

Kloakk said:
SkanskKorv said:
Is it normal that panaeolus bisporus mycelium changes the dye this much? I was worried about contams at first but after taking a good look it seems like the mycelium just ate the dye/ changed the hue which I've seen before, just not this aggressively. I've got the same goin on my ochra plates.



What % of DME are you using? If it is low and there is anything useful(it usually is) in the food color then the mycelium will digest it.


Oooh.... That makes a lot of sense then because I just switched to 0.5% lme in my agar recipe so I guess that solves it :rockon: thanks again bro!


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Re: Ask Quick Questions, Get Quick Answers [Re: SkanskKorv]
    #29289090 -

Would that mean dyed water agar would be less effective for outrunning contams?

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Re: Ask Quick Questions, Get Quick Answers [Re: The Ferryman] * 2
    #29289109 -

all mycelium seem to eat the dye which i why i dont bother adding it.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29289145 -



Here's some T2 plates of GT. I started with spores. Is the translucent growth normal or are all these contaminated?
Can I use any of these for inoculation of grain?

I'm still trying to understand how to use agar and what I'm looking at.


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Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Harpo67]
    #29289169 -

I would take another transfer from each. - I like to see symmetrical uniform growth.

Check this out:
https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap]
    #29289852 -

nocap said:
I would take another transfer from each. - I like to see symmetrical uniform growth.

Check this out:
https://www.shroomery.org/forums/showflat.php/Number/18430998#18430998


The one on the bottom right appears to be the best, or no?
Is the growth at 3 o'clock contamination or fuzzy mycellium growth? It appears on all of them in some form.

I will make some more transfer tonight.


--------------------
Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Harpo67]
    #29289856 -

Quick one, is there any verified difference between 12 on/12 off or 24/7 lighting during the grow?

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Re: Ask Quick Questions, Get Quick Answers [Re: Harpo67]
    #29289862 -

For me, it really depends on what I'm planning to do with the wedges. So if I'm wanting to take a culture to some sort of liquid, for example stasis storage or an LC, then I like to have very uniform growth before I do that.  Likewise, if I was planning to inoculate a lot of grains, for example  five or six jars for a monotub, then I would want to have that assurity from the uniform growth.

But, personally, I tend to fruit small projects that don't require a lot of grain. So in your case, what I would do, is I would take a transfer from the bottom right plate to another plate. And also put a wedge in a grain jar. And use that jar for a shoebox grow or something along those lines.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: DryySocks] * 1
    #29289870 -

DryySocks said:
Quick one, is there any verified difference between 12 on/12 off or 24/7 lighting during the grow?


Like most everyone, mycelium has circadian rhythms.

So I can imagine 24/7 being detrimental, but I'm not aware of any experiments actually verifying this.

I imagine if it is detrimental, immune health would be one of the first things affected.  As well as secondary metobolic processes that are less important to survival, such as biosynthesis of psilocybin.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: nocap]
    #29289907 -

I think it's pretty negligible for home growers maybe if u were some biotech company that needs to min max everything

I don't really give my shit controlled lighting or anything

I use 5000-6500k white/blue lighting but I don't keep track of the time I honestly usually leave it 24/7 so I don't got a fuck with it

And I leave my spawn in the dark just cuz I keep the lights off unless I'm in there


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: DryySocks] * 1
    #29289909 -

Got curious thanks to your question.  Learned fungi are a model organism for studying circadian rhythms.  So there's possibly even more relevant studies out there if you look.  But this one caught my eye.

Quote:
However, when grown in the laboratory under either constant light or dark conditions, spore
release ceased, indicating that this rhythm is driven by environmental cycles.


https://www.ias.ac.in/article/fulltext/jgen/075/03/0387-0401

I don't think it's healthy for the colonies reproductive cycle.  And as we're after the fruits of this cycle, I wouldn't recommend 24/7 light anymore than I would recommend growing in the dark.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29289919 -

I have two strains - Red Boy and Golden T.

Arrived at the same time.

The G.T. already gave the first flush and the R.B. not even a pin.

They have some white cloud like spots around but I dont know if it is Micelyum or contamination

Here are some pictures:






It does not smell but it is weird notting is growing.

Should I dunk it in water?

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Re: Ask Quick Questions, Get Quick Answers [Re: catnip40] * 1
    #29289924 -

catnip40 said:
I think it's pretty negligible for home growers maybe if u were some biotech company that needs to min max everything




I think it's one of those things like evaporation cycles in pans.  Sure you can get away without it, and still get enough fruits to trip, but fruit morphology will likely be affected.  As spores are intimitaly tied to fruits.

Plus, I enjoy taking photos of healthy fruits.  So I imagine I will min max fruit morphology for as long as I continue to take care of fungi. For me it's more about that than yield.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29289935 -

Blue light photomorphogenesis


--------------------

∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29289967 -

BeefSupremeJr said:
all mycelium seem to eat the dye which i why i dont bother adding it.


:whathesaid:

That and I like the clear stuff so any color that shouldn't be there really pops.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: Harpo67] * 1
    #29289968 -

Harpo67 said:


Here's some T2 plates of GT. I started with spores. Is the translucent growth normal or are all these contaminated?
Can I use any of these for inoculation of grain?

I'm still trying to understand how to use agar and what I'm looking at.


Transfer from 10 oclock on the bottom right plate, and five oclock on the bottom left plate.  See how that grows out.

I really like to see uniform growth before transferring to grain.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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