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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: fahtster] * 7
    #29126134 -

Another shoebox coming in.  Got some interesting cap colors on a few of them


Here’s a closer pic of the blue caps.  I thought they were aborting at first but they’ve continued to grow

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: TheGreatDellion] * 7
    #29126417 -

Mine are looking pretty scraggly so far, I upped the air so hopefully that helps.




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LAGM2020

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 999999999]
    #29126632 -

I like this idea of smaller tubes in a big tub, might have to try that!


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Networking my way to subtropicalis spores

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 999999999] * 2
    #29126690 -

999999999 said:


I thought I had mixed 2qt by 3 tubs with my feet, as usual, but now I see the reason of no pinning in that corner 🥴 … but these little fellows are undisturbed, for now


Very beautiful :heart::heart:

ps,
Are those gray containers from Clas Ohlson? If so, jævla kult :nyan:


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:ancientaliens:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: the_chosen_one] * 2
    #29126693 -

the_chosen_one said:
Last pics of this tray. Harvested last night.













Best wedding flower bouquet ever :heart::heart:
:nicesmile::trippinbawelz:


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:ancientaliens:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: fahtster]
    #29126695 -

fahtster said:
Nice.  I spawned my first semp clone zipblock today


Straight coir under fc 1:2.  Gonna add a casing at 100%



:solidnod::heart::heart:


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:ancientaliens:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: astra]
    #29127014 -

Looking for little advice.






Got spores from reputable vendor and sent them to agar.  These look clean to me.  All the growth looks similar. No weird colors.  Plan on doing at least 1 transfer before knocking up a bunch of grain and making a liquid culture.

My question is......I'm going out of town in 2 weeks and would like to time my activities such that something useful is going on when I'm gone.... Like maybe transfer to LC or grain right before we leave  or if I need to wait longer, do my first agar transfer right before we leave, but everything looks so consistent that I am hoping I can do my first transfer sooner rather than later.

I've read this thread and have a good idea what to do with the semps.  Still trying to figure out what do to with atl
... Not to side track the semp thread with stone stuff.  I'll go to that thread for that.  I just started all the plates at the same time with spore syringes from a reputable vendor.


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Hardhead] * 2
    #29127250 -

For how long will you be gone?

yeah it'd be good to have on grain before hand, if it's at least a week.

I'd probably let those colonies grow together and it should take off pretty quickly. 

And if you're feeling foggy start a small test jar when you take your xfer (if still looks clean)

Lc might not be the best route...:shrug:

Edited by LewDoja (02/15/25 04:15 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: LewDoja]
    #29127326 -

LewDoja said:
For how long will you be gone?

yeah it'd be good to have on grain before hand, if it's at least a week.

I'd probably let those colonies grow together and it should take off pretty quickly. 

And if you're feeling foggy start a small test jar when you take your xfer (if still looks clean)

Lc might not be the best route...:shrug:


Gone for 1 week.    Maybe i can do t1 middle of next week.  That will give it a week and change to grow.  Then I’ll send to grain right before we leave.    Should be ready to shake when we get back. 

Why not lc?    I’ve had great luck with other species.  Can always test lc on agar.    I never have though.  I just send it as long as solution is clear.  Had good luck so far.        I usually do stuff multiple ways in case my primary method fails.  Usually end up with fruits from both techniques.

My backup plan Ttbvi that I did lc to poo bag to humidity dome seed tray is still sitting up there cranking out fruits and I’ve neglected and abused that poor tray so bad it’s not even funny.  Last week it had water in bottom under the substrate.  Way too wet.  Figured it would’ve triched out by now.  I’ve left stems in it from harvest that rotted and it persists.  I got enough fruits off the primary grow to last a year.

I’m just anxious to get semps going.  I’ll tend them carefully till I get some fruits


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I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Hardhead] * 1
    #29127853 -

So, uh, had 5 tubs now go green, my culture is definitely clean but it’s been stupid hot here and I didn’t realise I should spawn a little dryer than standard till last night, reckon the heat and wetter substrate are the cause or should I focus elsewhere?


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Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 3.A.M] * 1
    #29127855 -

I'd say that's a starting point . 

Still have any plates of the culture ? Pics?

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Hardhead] * 1
    #29127858 -

Sounds like you've got this.

If your LC game is tight by all means. That comment was more aimed at the "if feeling froggy" part before t1 .

G/L
Safe travels on your trip.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: LewDoja] * 2
    #29127905 -

LewDoja said:
I'd say that's a starting point . 

Still have any plates of the culture ? Pics?


Goddamnit Lew, make me prove I’ve got clean plates, fuckin mutter, grumble, mutter fuckin…


I’m not sure what their preferred temps are but I’m betting 36c and humidity in the high 90’s aren’t helping.


--------------------

Clean MS from fresh fruits every time.
How I prep brown rice

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 3.A.M] * 1
    #29127920 -

3.A.M said:
LewDoja said:
I'd say that's a starting point . 

Still have any plates of the culture ? Pics?


Goddamnit Lew, make me prove I’ve got clean plates, fuckin mutter, grumble, mutter fuckin…


I’m not sure what their preferred temps are but I’m betting 36c and humidity in the high 90’s aren’t helping.


Not gonna lie... I went through SEVERAL green containers before deciding to split my jars berween bulk and myceliated grain. Semps can be tricky and the mycelium its magnificent at hiding embedded mold for some fucking reason lol This goes beyond cult skills, just plain voodoo nonsense that gets annoying but eventually you figure it out and well, semps are worth the extra effort 100% :heart::heart:

Your plates look a little off but then again my brain is starting to melt off from the sleeping meds hitting hard as i'm typing this. Hopefully i'm wrong and your cultures are flawless :mushroom2: (semp mycelium on agar looks like white mold anyway though)

ps,
If your current indoor temps are 36c with sky high humidity, give sterile sub a chance? Pretend they're pans for a minute, luv

:nicesmile::heart::heart:


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:ancientaliens:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: astra]
    #29128001 -

I've been thinking about contamination...

It seems to me that a very vulnerable time is when the bulk substrate is colonizing in open air, even though this may be done with a closed lid...there is still free air exchange.  You can do grain totally sterile and even to spawn to bulk sterile, but once you put the uncolonized bulk in free air....I think trich and other molds could sneak in there....Maybe not get a foothold for a while due to the overwhelming presence of mycelia, but give it a week or two and the mold rears its ugly head.....

My two large tubs of TTBVI got trich after a couple or few weeks and I'm thinking it probably got started during the open air colonization of bulk.  I did a sterile spawn to bulk transfer and mixed with sterilized instruments.  But after putting lid on in open air, all it takes is 1 mold spore and you are potentially done.

New idea is to do all in one bags.  Will sterilize bulk substrate in bag and then do spawn to bulk and reseal bag.  This way colonization can be sterile.  Should be able to case (with sterilized alkaline casing) immediately upon transfering to fruiting container.  Case......maybe let it sit with lid on for a couple days and then straight to fruiting chamber.

Just a thought here....only thing that has got me thinking about this, is my neglected seed tray of TTBVI in a humidity dome that I started in a bag......the thing just won't die....just keeps on keeping on.  I've started back trying to take care of it, but at one point I left the lid on it when it had fruits...every thing way too wet.  Was getting ready to throw it out.  Stems all fuzzy due to no fae.......Opened everything back up, resumed misting sides of dome and it is delivering the goods.......

Someone else on this thread talked about the all in one. AIO bags.  I don't claim any of this to be original thinking....still a noob here.


--------------------
I may not have stayed at a Holiday Inn Express, but I have taken 20 grams of fresh subtropicalis :smile:

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Hardhead] * 10
    #29128009 -



--------------------
Thank your postal workers when you see them. None of this would be possible without them.

Necessity is the mother of invention

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Hardhead] * 1
    #29128316 -

As long as your spawn is 100% colonized you don’t have to worry about much. Coir doesn’t have enough nutrients to go moldy, and properly prepared manure based sub should be resistant.

With pans it’s normal to get mold after a couple weeks, they have a very quick cycle compared to everything else.


Taking extra precautions won’t hurt, but it probably won’t make much difference either.


--------------------
LAGM2020

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a] * 8
    #29128361 -

These dry to toothpicks, 16 grams.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: 3.A.M] * 3
    #29128387 -

3.A.M said:
LewDoja said:
I'd say that's a starting point . 

Still have any plates of the culture ? Pics?


Goddamnit Lew, make me prove I’ve got clean plates, fuckin mutter, grumble, mutter fuckin…


I’m not sure what their preferred temps are but I’m betting 36c and humidity in the high 90’s aren’t helping.


That’s the kind of shit that stopped me growing in the summer.. can’t take the heartache

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 1
    #29128396 -

Just put spores on some plates this morning. Excited to get these going! I’ll be going back through this thread to make sure I have all my ducks in a row. Hopefully these are like my TTBVI and I get fruits my first tub. Been getting lucky after getting started last year.


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Hitchhikers guide to the Shroomery

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Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Grow Bags   North Spore Bulk Substrate

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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