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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 3
    #29727311 -

This has been a brilliant conversation to read and it super relevant to my current culture cleaning endeavors.

I had a very stubborn piece of myc that would not leave my scalple. I ended up using a pair of sterile tweezers to pull it off and drop it onto the agar.

Another thing I want to add to this conversation is what ive found from doing AM transfers directly to a trench. Post trench the myc becomes very aerial IME. Low nute AM transfers directly to a trench have bought be about a week of time before the biofilm makes it across the trench. So much so that im trying double trenching now and cutting 2 trenches in 1 plate to see how quickly the myc can get away from the biofilm.

I had a cabin produce a thick myc, I then put it through a 2nd low nute cabin, which ended up with the biofilm as well.

From a few other plates that were AM transfers directly to a trench, the biofilm did cross the trench, but about 5+ days after it appeared on the initial transfer zone. But the post trench biofilm was almost gone. I could barely see it. So I think im very close to being about to make an AM transfers directly to the center of a plate without biofilm showing up.


Biofilm right outside of the cabin and in a few other places though its hard to see in the picture.


This is the double trench I just did an AM to.
I think AM directly to trench is my new favorite cleaning method because ive been having good results.



Apparently biofilm on the initial transfer site vs very light biofilm post trench (hard to see in the photos but its still just barley there.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 1
    #29727406 -

Thank you all for sharing!

I do have a plate from an AM transfer. This is TTBVI AM transfer. It just took a little longer to start growing out. The wedges are pure experimentation. AM transfers have saved a couple cultures of mine. Thank you Faht for first showing me and that write up, clarifies what I am looking for! Thank you Kloakk for video and making this possible! And everyone else’s pictures have helped me understand some good ways to do things and what I am looking for.



I have also done an AM transfer of K-BVI from a MS LN plate. (You can see the myc growing in the middle where I first tried to get the tuft off the blade, but it fell right after, it was a big tuft from leading edge) (sorry I can’t open it right this second, my cat will destroy any chance I have at a clean culture. But I love her.)




The WA transfers looked the worst and all WA MS plates are showing yellow bacteria rings, so I only assume it was there when I took wedge transfers from the WA plate. There was no  AM on the WA plates to take from.


T1 from WA plate.




After using the warm mug yesterday to get rid of condensation, it all ended up on the AM, and now it looks like this. Is this biofilm? I plan on taking AM sample from edge (the edge is super fluffy around where it got wet and is now laid down) and put it to trench plate.


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*Not a therapist - Do not take advice from me

Be nice. You are loved. You deserve happiness. You are not alone.

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Re: Official Copelandia/Panaeolus Thread [Re: JoeTherapist]
    #29727420 -




Speaking of AM I'm still slightly untrained to the laying down/am these laying down or good?


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An enigma some caerulescens n a mono 😎

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Re: Official Copelandia/Panaeolus Thread [Re: bloodssonnew]
    #29727439 -

If you look at the side of the plate, you can tell if it’s laying flat or not. But those don’t look like it’s laying down to me :thumbup:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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