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Adas
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Re: Official Copelandia/Panaeolus Thread

[Re: HoodedBingling]

4
#29083461 - 01/10/25 12:06 PM (1 year, 9 months ago)

HoodedBingling said:

Anyone else got these kinds of yellow caps on bisps? Funny how they grow on that side. I didnt mix the all in one bags together so in that area mustve been the healthy one

They are just a bit dry. No worries.

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread

[Re: PuffM4gicDr4gon]

2
#29083595 - 01/10/25 02:16 PM (1 year, 9 months ago)

CaptainPuffy said:

I have some mycelium that I hope is pan, but could also just be mold. I'd like a 2nd opinion.

The source is from a bacterial BVI LC.

The top looked ugly AF, and I was about to throw it away as mold, but then I held it up to the light and saw different growth. What do you guys think? Is this worth taking a transfer from the leading edge?

The agar is only 1% LME. I've made up some .5 if the community thinks it's worth making some transfers.

This looks not good, if anything I would try to clean it up on BRF pucks or by using the trenching technique or just place a big wedge of this culture on one side of an empty dish, wait for the mycelium to grow out on the bare plate until it's safe to swab to fresh agar.

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Edited by Baba Yaga (01/10/25 02:33 PM)

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PuffM4gicDr4gon
Professional Coir Waster

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Re: Official Copelandia/Panaeolus Thread

[Re: Baba Yaga]
#29083613 - 01/10/25 02:38 PM (1 year, 9 months ago)

Baba Yaga said:

CaptainPuffy said:

I have some mycelium that I hope is pan, but could also just be mold. I'd like a 2nd opinion.

The source is from a bacterial BVI LC.

The top looked ugly AF, and I was about to throw it away as mold, but then I held it up to the light and saw different growth. What do you guys think? Is this worth taking a transfer from the leading edge?

The agar is only 1% LME. I've made up some .5 if the community thinks it's worth making some transfers.

This looks not good, if anything I would try to clean it up on BRF pucks or by using the trenching technique or just place a big wedge of this culture on one side of an empty dish, wait for the mycelium to grow out on the bare plate until it's safe to swab swap to fresh agar.

Thank you for the reply baba! I do have some fresh spore swabs of TTBVI coming from a sponsored vendor, so if this culture doesn't work out I'm not heartbroken about it.

I haven't tried the trenching technique, but I am familiar with making brf pucks and have all the equipment for that.

Could you provide more information about the swab swap procedure? By empty plate, do you mean a sterilized plate with 0 agar, or just a fresh blank plate? Also, what exactly is swab swap?

Even if I have spores coming, I'd still love to learn new techniques.

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Xclozure
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Re: Official Copelandia/Panaeolus Thread

[Re: PuffM4gicDr4gon]
#29083617 - 01/10/25 02:42 PM (1 year, 9 months ago)

Would yall make new coir if your bucket tek didnt have enough water. I was breaking up a couple handfuls of dry chunks next day and added more water.

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread

2
#29083634 - 01/10/25 03:03 PM (1 year, 9 months ago)

Should be good. If that happens to me I add some more water, if it comes out too wet I add some verm. Just make sure that the stuff needing proper pasteurization is on point.

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Xclozure
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Re: Official Copelandia/Panaeolus Thread

[Re: Baba Yaga]
#29083645 - 01/10/25 03:09 PM (1 year, 9 months ago)

Ty Yaga. I had a gut feeling just don't trust it sometimes lol :P

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread

[Re: PuffM4gicDr4gon]

3
#29083656 - 01/10/25 03:21 PM (1 year, 9 months ago)

CaptainPuffy said:

Thank you for the reply baba! I do have some fresh spore swabs of TTBVI coming from a sponsored vendor, so if this culture doesn't work out I'm not heartbroken about it.

I haven't tried the trenching technique, but I am familiar with making brf pucks and have all the equipment for that.

Could you provide more information about the swab swap procedure? By empty plate, do you mean a sterilized plate with 0 agar, or just a fresh blank plate? Also, what exactly is swab swap?

Even if I have spores coming, I'd still love to learn new techniques.

Lol, sorry. That was a typo which I have edited out now. I meant to say just "swab". Its just like the trenching technique in principle but you place a wedge onto an empty plate and wait for the mycelium to grow across the bare plastics and then transfer it to a new agar plate. I remember someone doing this in glass jars but I can't remember who, feel like it was smelly hobbit or yoshi trainer.

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PuffM4gicDr4gon
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Re: Official Copelandia/Panaeolus Thread

[Re: Baba Yaga]
#29083663 - 01/10/25 03:25 PM (1 year, 9 months ago)

Thanks baba! I'll probably do both. Do the empty plate, grab some mycelium, transfer to agar, let it grow a bit and then put that in a trench'd brf puck. If for nothing else than to try something new!

Edit: I'm just saw that the linked thread is with trenched agar, so I guess I can save a step

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Edited by PuffM4gicDr4gon (01/10/25 03:33 PM)

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Dala
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Re: Official Copelandia/Panaeolus Thread

1
#29083667 - 01/10/25 03:29 PM (1 year, 9 months ago)

Baba Yaga said:

...or just place a big wedge of this culture on one side of an empty dish, wait for the mycelium to grow out on the bare plate until it's safe to swab to fresh agar.

Wow. I'd read about placing a wedge on the empty side of a half-poured agar plate and letting it reach out to colonize the agar on the other side, then transferring from there, but what you're doing is beautifully simple. Thanks for sharing.

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread

[Re: PuffM4gicDr4gon]
#29083674 - 01/10/25 03:42 PM (1 year, 9 months ago)

TBH I only have used BRF pucks so far and have not tried the trenching or empty plate method yet :blush:

:blush:

but I will the next time I have to perform a cleanup.

CaptainPuffy said:

Thanks baba! I'll probably do both. Do the empty plate, grab some mycelium, transfer to agar, let it grow a bit and then put that in a trench'd brf puck. If for nothing else than to try something new!

That's the spirit :rockon:

:rockon:

--------------------
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lillFish
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Re: Official Copelandia/Panaeolus Thread

[Re: 3.A.M]
#29083727 - 01/10/25 04:43 PM (1 year, 9 months ago)

3.A.M said:

Yeah, I was experimenting with a different mix and method of pasteurisation and all my tubs got rubbery bruised overlay, going back to tried and true for my next lot.

What does that kind of rubbery overlay indicate? I have seen that on one of my trays that is refusing to pin.

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Mackload
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Re: Official Copelandia/Panaeolus Thread

[Re: Baba Yaga]

1
#29083742 - 01/10/25 04:56 PM (1 year, 9 months ago)

nice new avatar :hellyeah:

:hellyeah:

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Baba Yaga
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Re: Official Copelandia/Panaeolus Thread

1
#29083751 - 01/10/25 05:03 PM (1 year, 9 months ago)

Thanks

:awedance:

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3.A.M
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Re: Official Copelandia/Panaeolus Thread

9
#29083766 - 01/10/25 05:13 PM (1 year, 9 months ago)

@lillfish
I’d assume maybe bacterial or I just cased them too early and should’ve cased again :shrug:

:shrug:

Pans are still mostly a mystery to me and like to buck convention, the fact I’m growing in tubs might have something to do with it also.

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Edited by 3.A.M (01/11/25 02:41 AM)

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Bra
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Re: Official Copelandia/Panaeolus Thread

[Re: 3.A.M]

10
#29084549 - 01/11/25 09:29 AM (1 year, 9 months ago)

Pan cyanescens Peace River

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Smellyhobbit
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Re: Official Copelandia/Panaeolus Thread

[Re: Bra]

8
#29085224 - 01/11/25 06:34 PM (1 year, 9 months ago)

Spawn check. Sending these way before anyone answers but I’d still like to hear opinions. Took 21 days to colonize from teeny weenie wedges.

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Henry Swanson

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Re: Official Copelandia/Panaeolus Thread

[Re: Smellyhobbit]

3
#29085233 - 01/11/25 06:44 PM (1 year, 9 months ago)

Looks good to me. :rockon:

:rockon:

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Smellyhobbit
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Re: Official Copelandia/Panaeolus Thread

[Re: Henry Swanson]

8
#29085291 - 01/11/25 07:13 PM (1 year, 9 months ago)

Let’s rock. First pan cyans here i come

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PancyanterA
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Re: Official Copelandia/Panaeolus Thread

[Re: Smellyhobbit]
#29085428 - 01/11/25 08:48 PM (1 year, 9 months ago)

Hell yeah! What is that on?

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Smellyhobbit
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Re: Official Copelandia/Panaeolus Thread

[Re: PancyanterA]
#29085536 - 01/11/25 10:04 PM (1 year, 9 months ago)

PancyanterA said:

Hell yeah! What is that on?

Sorghum

--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:

:kodama:

Anyone can get a canopy. But can you get what you want?

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