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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
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Re: Ask Quick Questions, Get Quick Answers

1
#29077711 - 01/05/25 05:51 AM (1 year, 10 months ago)

What size filter?

How much Do those weigh?

What was your prep method?

Are those 2 bags each or the same bag both pictures (of each)?

Edited by LewDoja (01/05/25 05:54 AM)

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Milan174
Mr

Registered: 12/04/24
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Loc: Serbia
Last seen: 1 year, 9 months

Re: Ask Quick Questions, Get Quick Answers

[Re: LewDoja]
#29077734 - 01/05/25 06:44 AM (1 year, 10 months ago)

If you mean filter permeability not sure,as those are "chinese" bags😂
5lb bags,but filled halfway so maybe 2.5lb of total Substrate.
I hidrated pallets to 55-60%,put in bag,hidrated an cooked wheat grains,put on top an then sterilized in presurecooker for 2h.
Inoculated with agar plate on top.
After aprox 1.5 to 2 weeks grain was complete taken by micelium but then it stoped to progress.
Pioppino i shaked and you can see it is trying to progress to sawdust around grains but extremely slow and it is struggling(i am guessing by micelium piss😂
First two pics are maitake bag-top where grain is and bottom to see there is no change.
Second two are same pioppino bag-bottom with few mic islands around individual grains and top where it is thriving.
Is it possible that issue is with fuel pallets?
They add something or maybe it is not hardwood as they are claiming?

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
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Re: Ask Quick Questions, Get Quick Answers

[Re: Milan174]
#29077739 - 01/05/25 07:03 AM (1 year, 10 months ago)

Do you always keep the bag tops rolled down tight to the grain like that? I like to keep as much airspace in the bags as possible to give good gas exchange. If the filter is rolled up inside the creases maybe it’s starved for air?

--------------------
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Kloakk
Strange

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Re: Ask Quick Questions, Get Quick Answers

[Re: RoscoeReturns]
#29077755 - 01/05/25 07:27 AM (1 year, 10 months ago)

Should I do one last brake&shake or just LITFA?

--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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Milan174
Mr

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Re: Ask Quick Questions, Get Quick Answers

[Re: RoscoeReturns]
#29077756 - 01/05/25 07:30 AM (1 year, 10 months ago)

RoscoeReturns said:

Do you always keep the bag tops rolled down tight to the grain like that? I like to keep as much airspace in the bags as possible to give good gas exchange. If the filter is rolled up inside the creases maybe it’s starved for air?

I will try that,it will not harm for sure.
Thanks

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LewDoja
mad $cientist, ganjacologist

Registered: 09/18/09
Posts: 4,849
Loc: A fictional tale, in fictional...
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Re: Ask Quick Questions, Get Quick Answers

[Re: Kloakk]

2
#29077757 - 01/05/25 07:31 AM (1 year, 10 months ago)

I'd wait till 100.01% to shake. That way you know for sure it was fully colonized and can spawn a day two when signs of recovery.

Edited by LewDoja (01/05/25 07:32 AM)

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Kloakk
Strange

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Re: Ask Quick Questions, Get Quick Answers

[Re: LewDoja]

1
#29077760 - 01/05/25 07:39 AM (1 year, 10 months ago)

LewDoja said:

I'd wait till 100.01% to shake. That way you know for sure it was fully colonized and can spawn a day two when signs of recovery.

:takingnotes:

Got it, thanks and congratulation on the new TC tag :rockon:

:rockon:

--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett

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bluespirit13
Getting Back Into It

Registered: 12/06/24
Posts: 467
Loc: USA
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Re: Ask Quick Questions, Get Quick Answers

[Re: Kloakk]
#29077872 - 01/05/25 09:58 AM (1 year, 10 months ago)

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?

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Axman
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Re: Ask Quick Questions, Get Quick Answers

[Re: bluespirit13]
#29077877 - 01/05/25 10:03 AM (1 year, 10 months ago)

bluespirit13 said:

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?

A lot of people will fill a jar/shot glass with distilled water, put foil over it then pierce the foil with the syringe's (closed syringe, no water or air inside) needle. Then wrap in foil, and PC. Once the PC cycle is done you can then pull the sterile distilled water out of the jar (that the needle is pierced into) and into the syringe.

IIRC if you put a syringe with water into the PC the water inside the syringe can boil and possibly cause the syringe to lose fluid. That may be what caused your problem.

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The Tao
Read more, post less.

Registered: 09/12/19
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Loc: Canada

Last seen: 3 hours, 8 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: bluespirit13]
#29077886 - 01/05/25 10:07 AM (1 year, 10 months ago)

What was your process?

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bluespirit13
Getting Back Into It

Registered: 12/06/24
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Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]
#29077910 - 01/05/25 10:38 AM (1 year, 10 months ago)

I put water into a syringe, capped it, and then wrapped it in foil, then pc for 30 mins

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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,848

Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]
#29077912 - 01/05/25 10:39 AM (1 year, 10 months ago)

FWIW, when I’ve been in quick need of “sterile” water with none on hand, using an SAB or FFU, I pour some water from an iso-wiped sealed bottle of drinking water (or distilled, whatever I’ve got) into a test tube or vial and then aspirate into a sterile syringe and use it from there. It’s a shortcut that is not bulletproof and has potential to bite ya in the ass, but in my own experience, I’ve not yet encountered any contaminations over this past year. That said, I don't make spore syringes or prepare stuff for others and am typically just hydrating spores for a bit before putting them to agar for cleanup (so, i’m expecting it to be dirty/non-sterile from the outset). I’ve never bothered
to plate straight water using this approach…probably should though.

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Stuntbum
Reader

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Re: Ask Quick Questions, Get Quick Answers

[Re: flapping_bird]

8
#29077915 - 01/05/25 10:42 AM (1 year, 10 months ago)

First Nat tub. Harvest now or let em ride a little longer?

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flapping_bird
Human, Be-ing

Registered: 01/03/25
Posts: 1,848

Re: Ask Quick Questions, Get Quick Answers

[Re: Stuntbum]
#29077918 - 01/05/25 10:46 AM (1 year, 10 months ago)

nice tub-o-nats! Beautiful. Congrats!

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Peace to you, friend!
The HHG is a guide to getting started.
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ThurmanMurman
Sandwich Artist

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Re: Ask Quick Questions, Get Quick Answers

[Re: bluespirit13]
#29077996 - 01/05/25 12:14 PM (1 year, 10 months ago)

bluespirit13 said:

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?

Did you have the needle pointing up in the PC? If not, try that.

--------------------

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Shine down upon the severed
Shine until the two become one

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,773
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers

2
#29078039 - 01/05/25 12:51 PM (1 year, 10 months ago)

Stuntbum said:

First Nat tub. Harvest now or let em ride a little longer?

I'd harvest that.

--------------------
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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Churpee Choozle
Crusted Cultinator

Registered: 11/11/24
Posts: 3,029
Last seen: 9 hours, 22 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: cybork]
#29078067 - 01/05/25 01:09 PM (1 year, 10 months ago)

Shit dat wordt voor het eerst in 1000 jaar naar de Dirk

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cybork
Stdt mycology

Registered: 07/01/23
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Re: Ask Quick Questions, Get Quick Answers

[Re: ThurmanMurman]
#29078076 - 01/05/25 01:16 PM (1 year, 10 months ago)

I think the water will boil and expand. And as such water will come out via the needle, right?

ThurmanMurman said:

bluespirit13 said:

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?

Did you have the needle pointing up in the PC? If not, try that.

I think the water in the syringe will boil and expand. And as such water will come out via the needle, right? Regardless of it standing up.

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PuffM4gicDr4gon
Professional Coir Waster

Registered: 06/09/11
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Re: Ask Quick Questions, Get Quick Answers

[Re: cybork]

2
#29078082 - 01/05/25 01:19 PM (1 year, 10 months ago)

cybork said:

I think the water will boil and expand. And as such water will come out via the needle, right?
ThurmanMurman said:

bluespirit13 said:

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?

Did you have the needle pointing up in the PC? If not, try that.

I think the water in the syringe will boil and expand. And as such water will come out via the needle, right? Regardless of it standing up.

I've sterilized water in a needle several times. Put the cap on, point it so the needle is facing up wrap it in tin foil and it works. You lose a couple milliliters but not much.

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Radamanto
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Re: Ask Quick Questions, Get Quick Answers

[Re: cybork]
#29078084 - 01/05/25 01:20 PM (1 year, 10 months ago)

Yes water will probably come out in every scenario, but by PCing the syringes with the neede upright only a little bit of water will boil over. If you PC them horizontally the syringe will be almost empty when you take it out of the PC. (At least this is what my personal experience has been with both options)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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