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nektar61
Heterotroph



Registered: 07/04/20
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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] 1
#29072250 - 12/30/24 11:58 AM (17 days, 9 hours ago) |
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Quote:
KlarnEvil9 said: QQ: How should I proceed with this BRF puck? We were away for a couple of days and this morning I found this:

The other day I could see the teeny-tiny cluster starting to form, it was exciting. But now I realize that I only kinda have a plan.
Should I just pop the puck out and slap 'er in a smallish ziploc? The other x7x+ puck looks to ba a few days behind.
Thank you all for your expertise.
I haven't grow with pucks. But personally I'd open the lid and let them grow and open up, it will look very cool and make a nice picture.
Plus I just Googled. Apparently x7x+ are a type of Psilocybe cubensis mushrooms, so you can eat them after you photograph them.
I've heard they're non-toxic, and actually make you feel happy, and make the walls wiggle a little.
But I'd take the tallest one or widest one and do a spore print before eating. That way you can grow more later!
(it's Monday, I'm at work, and didn't sleep enough. But seriously, that's what I'd do with those.
Is your user name an ELP reference? I like them.
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,297
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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] 1
#29072263 - 12/30/24 12:03 PM (17 days, 9 hours ago) |
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Quote:
KlarnEvil9 said: QQ: How should I proceed with this BRF puck? We were away for a couple of days and this morning I found this:

The other day I could see the teeny-tiny cluster starting to form, it was exciting. But now I realize that I only kinda have a plan.
Should I just pop the puck out and slap 'er in a smallish ziploc? The other x7x+ puck looks to ba a few days behind.
Thank you all for your expertise.
Isn't the whole purpose of growing on pucks like that to have a clean way to grab pins and then clone them? Grab one with a clean pair of tweezers then put it on agar or another petri puck and then, when it seems contam free, grow it out.
-------------------- Matter is spirit moving slowly enough to be seen.
—Pierre Teilhard de Chardin
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nektar61
Heterotroph



Registered: 07/04/20
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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca] 1
#29072272 - 12/30/24 12:07 PM (17 days, 9 hours ago) |
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Quote:
KauaiOrca said:
Quote:
KlarnEvil9 said: QQ: How should I proceed with this BRF puck? We were away for a couple of days and this morning I found this:

The other day I could see the teeny-tiny cluster starting to form, it was exciting. But now I realize that I only kinda have a plan.
Should I just pop the puck out and slap 'er in a smallish ziploc? The other x7x+ puck looks to ba a few days behind.
Thank you all for your expertise.
Isn't the whole purpose of growing on pucks like that to have a clean way to grab pins and then clone them? Grab one with a clean pair of tweezers then put it on agar or another petri puck and then, when it seems contam free, grow it out.
If that's the case, pluck it before any of them drop spores or there will be a chance it won't be a clone. Also, cut off the cap, (same reason) and base (to keep from having mycleum of more than one other shrooms).
That's how you get a real clone from a pin (or plin).
You could do that and still leave the lid off and let it grow out.
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KlarnEvil9
Spoot the juice!!!



Registered: 06/13/24
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] 1
#29072273 - 12/30/24 12:08 PM (17 days, 9 hours ago) |
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Thank you for the info!
I popped the puck out and put it on a lid, in a small container, with a little water at the bottom. Then I put all that in a ziploc bag w/ holes (fahtlock style). I have some more samples of this culture in various stages, but I'm absolutely going to print the big boi. Seems to be growing from the little cluster that was there first.
And yes, my username is definitely an ELP reference. One of my favorite bands of all time! And I was probably listening to Brain Salad Surgery when I made my account...
-------------------- I am Klarn of the Cosmos, ninth of his name! Keeper of the green plates, protector of arachnids!
(and killer of threads!)
Turns out that I'm an amateur at everything...
Read more, post less! LAGM 2025
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KlarnEvil9
Spoot the juice!!!



Registered: 06/13/24
Posts: 549
Loc: Upper Earth
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Re: Ask Quick Questions, Get Quick Answers [Re: KauaiOrca] 1
#29072279 - 12/30/24 12:12 PM (17 days, 9 hours ago) |
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Well, I was kinda trying to do both - clean up a culture and let it grow out. I'd like to clone and print that big one, but I'd also like to grow a few fruits while I wait on grain jars.
@Nektar61 - good advice on cloning, I had not considered the idea of physically seperating it from the substrate to help differentiate its genetics from its neighbors.
-------------------- I am Klarn of the Cosmos, ninth of his name! Keeper of the green plates, protector of arachnids!
(and killer of threads!)
Turns out that I'm an amateur at everything...
Read more, post less! LAGM 2025
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Jrey16
Magic Kabute



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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#29072283 - 12/30/24 12:14 PM (17 days, 9 hours ago) |
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Has anyone ever put two to three fully colonized 90mm agar plates into a jar of grains just for the fuck if it? Or to speed up colonization time? I'm about to inoculate right now and I have a bunch of agar plates. Thinking about putting 2, or even 3 if it fits in each grain jar lol. Would also like to speed up colonization time
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NeitherHere
Lucid dreamer and ClusterHead


Registered: 12/25/24
Posts: 27
Loc: NorThere
Last seen: 3 days, 23 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
#29072284 - 12/30/24 12:14 PM (17 days, 9 hours ago) |
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I'm backed into a corner and could use some help. I'm low on supplies and financially fucked after having my identity stolen and losing a job. I'm pretty sure I got myself a great salary position in the works at the moment but I'm really low on supply and I can't afford to fuck up this batch. I have serious cluster headaches and I'm almost out of fungi.
I have a jar of hydrated coir in the oven at 165 for 4 hours. usually I just put boiling water on it and don't bother with pasteurization but I'm out of grain and can't make any serious fuck ups here.
am I being overly cautious with this? should I just go to colonizing it rather than wasting time with pasteurization?
can I colonize quinoa and/or oat meal to get my next batch of tampanensis or cubensis started? they're the only options I have at my disposal at the moment.
any guidance would help here. I don't need snarky remarks about my situation or what I should be doing with my life, I'm working on what I can. fucking this up could mean several weeks without actual pain relief and it'll absolutely fuck my start with what could essentially be a dream job if I can't function because of the cluster attacks..
before anyone says anything, the medications do very little and I lost my insurance when I lost my job so I'm fucked on that front too...
-------------------- Every day is a gift
Lucid dreamer, Cluster head, and part time Librarian.
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nektar61
Heterotroph



Registered: 07/04/20
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Re: Ask Quick Questions, Get Quick Answers [Re: KlarnEvil9] 1
#29072289 - 12/30/24 12:17 PM (17 days, 9 hours ago) |
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Quote:
KlarnEvil9 said: @Nektar61 - good advice on cloning, I had not considered the idea of physically seperating it from the substrate to help differentiate its genetics from its neighbors.
I haven't heard anyone say that but it absolutely makes sense.
I'd do it now, before any drop spores. Then leave the top open, they're getting fuzzier feet then I've seen with that variety, which may need they're not getting enough FAE. Are there holes in the lid?
A lot of people cut open mature shrooms' stem to get clone material, but under a microscope, I've seen spores on those if the spores have dropped.
They weren't in there before I cut it open, I'm sure, but spores are so light and tiny it's probably impossible not to have them all over a sample, even just from the air when cutting it open.
I did not take pix of that, but here's just some general spore pix I took:
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QuantumZ00
Endospore Explorer



Registered: 07/11/24
Posts: 3,221
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
#29072293 - 12/30/24 12:20 PM (17 days, 9 hours ago) |
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Quote:
nektar61 said: Quote:
Smellyhobbit said:
Quote:
QuantumZ00 said: QQ: After drying mushrooms in dehydrator and putting desiccant silica gel packs inside the bags with them and placing them in an airtight container...
...where is the "best practices" place to store the container they're in (for slowest loss of potency over time)? In a room below 70 degrees temperature, or in a fridge, or freezer, or on any shelf as long as temps don't exceed X?
Room temp, glass jar, away from light.
I do room temp, away from light, but vacuum sealed, with no desiccant. Haven't done extremely long term test, but recently ate some that I packed that method 2 years and 9 months ago, they were as strong as the day I packed them.
There's a thread about that on here: https://www.shroomery.org/forums/showflat.php/Number/27660402/fpart/1/vc/1
Oh that's convenient because I stole a vacuum sealer from my old job when they fired me for some bullshit
Should still work like a charm too
-------------------- Endlessly endorsing empathy empirically energizing existence™
~Always practice radical kindness~
R.I.P. Betty Boop, the Greatest Mother who ever lived. October 1961-November 2024.
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nektar61
Heterotroph



Registered: 07/04/20
Posts: 3,686
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Re: Ask Quick Questions, Get Quick Answers [Re: NeitherHere] 1
#29072300 - 12/30/24 12:27 PM (17 days, 9 hours ago) |
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Quote:
NeitherHere said: ...I have serious cluster headaches and I'm almost out of fungi.
I have a jar of hydrated coir in the oven at 165 for 4 hours. usually I just put boiling water on it and don't bother with pasteurization but I'm out of grain and can't make any serious fuck ups here.
am I being overly cautious with this? should I just go to colonizing it rather than wasting time with pasteurization?
can I colonize quinoa and/or oat meal to get my next batch of tampanensis or cubensis started? they're the only options I have at my disposal at the moment. ...
I don't think shroom will grow on coir alone. Not enough nutrients. But quinoa is a grain, I don't see why that wouldn't work.
I just pour boiling water on coir and let it sit.
Here's a post on growing shrooms on quinoa, and there are others here if you use advanced search. https://www.shroomery.org/forums/showflat.php/Number/27251040
That one said it colonized twice as fast as rice.
If you colonize on quinoa, but you run out before you get shrooms grown, colonized shroom mycelium has some psilo. Not as much as shrooms, but if you're microdosing, it should work.
Edited by nektar61 (12/30/24 12:38 PM)
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KauaiOrca
Waterman

Registered: 08/12/08
Posts: 3,297
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] 1
#29072302 - 12/30/24 12:30 PM (17 days, 9 hours ago) |
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Quote:
KauaiOrca, who's posting on this thread, grows shrooms without a pressure cooker, if he doesn't see this and offer suggrestions, maybe DM him.
Actually, I always use a pressure cooker, so I'm not the one to ask about that.
-------------------- Matter is spirit moving slowly enough to be seen.
—Pierre Teilhard de Chardin
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Henry Swanson

Registered: 08/05/24
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Loc: Little China
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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
#29072303 - 12/30/24 12:31 PM (17 days, 9 hours ago) |
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Quote:
ThurmanMurman said:
Quote:
nektar61 said:
Quote:
Scemilope said: I had poor results from vacuum sealing. I had cheapo vac bags, better bags would probably help. I store in glass jars or mylar zipper pouches, at room temp in a dark closet. I toss a desiccant pack in, but it's probably optional.
The bags I use do maintain vacuum. Did yours not?
QuantumZ00; One thing I would not recommend for anyone looking for long term storage, no matter how you store it, is grinding it into a powder. I've heard of people doing that to save space.
That increases the surface area, which will get oxygen into more of it, which makes psilocin (not psilocybin) inert with time.
Store shrooms as full shrooms, or stems and caps, as intact as you can get them in the dehydrator.
I dehydrate at 165 to 185 F (two different dehydrators, both are fine) for 24 hours until cracker dry, so they snap (not bend) if you bend them.
A lot of new people on here think that heat will "hurt the potency", it will not at those temps. Potency will be more likely ruined by being stored not totally dry.
So I've seen this mentioned before about powdered storage and it makes sense. I saw there was someone who did a long term storage test and his results saw the capsules he made holding up the best and Im curious to know if the capsules are the ticket to the preservation there or if it was some other variable. I just made about 300 capsules to save space, so I guess we'll see.
I capsulize mine as well, and I’ve seen the thread you’re talking about and some people who submitted old capsules for testing and it looks like they hold up very well. So between that the space savings and the convenience of dosing It’s how I’ll be continuing to store my fruits.
With vac bags you have to be careful what type of bag you get. Polyethylene bags allow oxygen to permeate very quickly. PA or PA/PE bags hold up much better, but I’d still feel better storing in a airtight glass container.
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nektar61
Heterotroph



Registered: 07/04/20
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Re: Ask Quick Questions, Get Quick Answers [Re: Jrey16]
#29072307 - 12/30/24 12:37 PM (17 days, 9 hours ago) |
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Quote:
Jrey16 said: Has anyone ever put two to three fully colonized 90mm agar plates into a jar of grains just for the fuck if it? Or to speed up colonization time? I'm about to inoculate right now and I have a bunch of agar plates. Thinking about putting 2, or even 3 if it fits in each grain jar lol. Would also like to speed up colonization time
No. I don't know that it would speed it up if you put two on the top of the jar. Colonization is sped up by more points of the grain touching the mycelium agar.
If you're in a hurry I'd fill jars about half full of grain, maybe 2/3, chop up one plate into 4 pieces, put in the jar, close, then roll the jar edgewise, and sideways, to get the agar all over it. Might work better with two, but that rolling will get it around more.
If you just have extra done plates, I'd put a few in a baggie and put in the fridge (not freezer) you can use later without growing more. I've sent one to grain up to a year later. (If they don't dry out.)
They'll actually continue to grow in fridge but very slowly. Like in a year they'll grow what they'd grow in a couple weeks at room temp.
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Nichrome
Participle Phrase



Registered: 12/17/18
Posts: 7,669
Loc: Zone 5
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Re: Ask Quick Questions, Get Quick Answers [Re: NeitherHere] 3
#29072309 - 12/30/24 12:37 PM (17 days, 9 hours ago) |
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Quote:
NeitherHere said: I'm backed into a corner and could use some help. I'm low on supplies and financially fucked after having my identity stolen and losing a job. I'm pretty sure I got myself a great salary position in the works at the moment but I'm really low on supply and I can't afford to fuck up this batch. I have serious cluster headaches and I'm almost out of fungi.
I have a jar of hydrated coir in the oven at 165 for 4 hours. usually I just put boiling water on it and don't bother with pasteurization but I'm out of grain and can't make any serious fuck ups here.
am I being overly cautious with this? should I just go to colonizing it rather than wasting time with pasteurization?
can I colonize quinoa and/or oat meal to get my next batch of tampanensis or cubensis started? they're the only options I have at my disposal at the moment.
any guidance would help here. I don't need snarky remarks about my situation or what I should be doing with my life, I'm working on what I can. fucking this up could mean several weeks without actual pain relief and it'll absolutely fuck my start with what could essentially be a dream job if I can't function because of the cluster attacks..
before anyone says anything, the medications do very little and I lost my insurance when I lost my job so I'm fucked on that front too...
Quinoa and oatmeal are both very mucilaginous and are very very poor substances to use for spawn due to their physical nature and the load of phytic acid in quinoa. If those are the only carbohydrate sources someone has to work with, PF cakes made with either of those, ground in a coffee grinder, would work best and probably quite well. Using those grains as is with the methodology you are describing will be a nightmare and will likely only contribute to headaches.
That being said if someone is growing just for personal headache medicine, growing semperviva mycelium on grain, letting it sit for about 6 weeks, and dehydrating the aged colonized grain will give a large abundance of very good quality substance that is as effective in every way as cube or tamp fruits. Fruits are an extra step and a hard one without exp. If medicine is the go to, growing potent mycelium on grain is a one step, very effective process. Semperviva is the right species. Other species that grow nice potent myc are caerulescen, azurescen, and zapotecorum.
-------------------- discussions are a healthy alternative to arguments
There is only one electron, and it's you.
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nektar61
Heterotroph



Registered: 07/04/20
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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] 2
#29072312 - 12/30/24 12:41 PM (17 days, 9 hours ago) |
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Quote:
Henry Swanson said: With vac bags you have to be careful what type of bag you get. Polyethylene bags allow oxygen to permeate very quickly. PA or PA/PE bags hold up much better, but I’d still feel better storing in a airtight glass container.
The brand I used that held up well are called FoodSaver.
They're not bags, they're a continuous roll, and my dehydrator cuts them on both ends and then seals the ends with heat. You manually cut them with a built-in blade, then seal one end, fill them, then put it back in the machine and it sucks out the air and seals at the same time.
The sealer I use is a brand called TKTK.
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Mr Piggy
Playing "queer pass"



Registered: 09/29/11
Posts: 11,962
Loc: bioilliminicent©️
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] 3
#29072316 - 12/30/24 12:46 PM (17 days, 9 hours ago) |
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I’ve had too many vac bags of that brand and more fail on me. The only sure way I have found to keep them long term is sealing glass jars such as quart jars with the original two piece lids.
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻
New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery
Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."
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Henry Swanson

Registered: 08/05/24
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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] 1
#29072318 - 12/30/24 12:47 PM (17 days, 9 hours ago) |
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Same for ours, we bought a sealer way back when we first got a sous vide, all of ours are rolls too. But our sealer is a cheapy one so we have to cut the roll ourselves.
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Mr Piggy
Playing "queer pass"



Registered: 09/29/11
Posts: 11,962
Loc: bioilliminicent©️
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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] 1
#29072320 - 12/30/24 12:48 PM (17 days, 9 hours ago) |
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I don’t even put my venison in them, sausage and fish only
-------------------- 🅃🄴🄰🄼 🄵🄾🄸🄻
New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery
Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."
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AspectOfTheCreator
Mastering the Art of Success



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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy]
#29072332 - 12/30/24 12:57 PM (17 days, 8 hours ago) |
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I know I asked a little while ago but can I declare my lids, grain prep, and pc process a success now? Theres some starchy crud on inside glass from burst grains but it seems clean.
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NeitherHere
Lucid dreamer and ClusterHead


Registered: 12/25/24
Posts: 27
Loc: NorThere
Last seen: 3 days, 23 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
#29072337 - 12/30/24 01:02 PM (17 days, 8 hours ago) |
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I had some semperviva spores laying around but this last year something contaminated my entire library of spores so I'm starting from scratch here. at the moment I have "blue kachina" and avery albino, I suspect they're the exact same thing and that the BK seller just rebranded it because they look EXACTLY the same. and a tampanensis culture.
I'm looking at getting semperviva and pancyans. I tried doing azurescence but it's not compatible with my region so it was an abject failure.
at the moment I have a pint of "spent" tampanensis grains I'm thinking about making a tea with and turning into ReverendMyc gummies.
I'm going to take my coir out and mix it with my currently inoculated grains.
thank you for your assistance and the other member above you (I've already forgotten names)
-------------------- Every day is a gift
Lucid dreamer, Cluster head, and part time Librarian.
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