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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Milan174] * 1
    #29077711 -

What size filter?

How much Do those weigh?

What was your prep method?

Are those 2 bags each or the same bag both pictures (of each)?

Edited by LewDoja (01/05/25 05:54 AM)

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29077734 -

If you mean filter permeability not sure,as those are "chinese" bags😂
5lb bags,but filled halfway so maybe 2.5lb of total Substrate.
I hidrated pallets to 55-60%,put in bag,hidrated an cooked wheat grains,put on top an then sterilized in presurecooker for 2h.
Inoculated with agar plate on top.
After aprox 1.5 to 2 weeks grain was complete taken by micelium but then it stoped to progress.
Pioppino i shaked and you can see it is trying to progress to sawdust around grains but extremely slow and it is struggling(i am guessing by micelium piss😂
First two pics are maitake bag-top where grain is and bottom to see there is no change.
Second two are same pioppino bag-bottom with few mic islands around individual grains and top where it is thriving.
Is it possible that issue is with fuel pallets?
They add something or maybe it is not hardwood as they are claiming?

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Re: Ask Quick Questions, Get Quick Answers [Re: Milan174]
    #29077739 -

Do you always keep the bag tops rolled down tight to the grain like that? I like to keep as much airspace in the bags as possible to give good gas exchange. If the filter is rolled up inside the creases maybe it’s starved for air?


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29077755 -

Should I do one last brake&shake or just LITFA?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29077756 -

RoscoeReturns said:
Do you always keep the bag tops rolled down tight to the grain like that? I like to keep as much airspace in the bags as possible to give good gas exchange. If the filter is rolled up inside the creases maybe it’s starved for air?


I will try that,it will not harm for sure.
Thanks

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29077757 -

I'd wait till 100.01% to shake. That way you know for sure it was fully colonized and can spawn a day two when signs of recovery.

Edited by LewDoja (01/05/25 07:32 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29077760 -

LewDoja said:
I'd wait till 100.01% to shake. That way you know for sure it was fully colonized and can spawn a day two when signs of recovery.


:takingnotes: Got it, thanks and congratulation on the new TC tag:rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29077872 -

I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?


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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13]
    #29077877 -

bluespirit13 said:
I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?


A lot of people will fill a jar/shot glass with distilled water, put foil over it then pierce the foil with the syringe's (closed syringe, no water or air inside) needle. Then wrap in foil, and PC. Once the PC cycle is done you can then pull the sterile distilled water out of the jar (that the needle is pierced into) and into the syringe.

IIRC if you put a syringe with water into the PC the water inside the syringe can boil and possibly cause the syringe to lose fluid. That may be what caused your problem.


--------------------
The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13]
    #29077886 -

What was your process?

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29077910 -

I put water into a syringe, capped it, and then wrapped it in foil, then pc for 30 mins


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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29077912 -

FWIW,  when I’ve been in quick need of “sterile” water with none on hand, using an SAB or FFU, I pour some water from an iso-wiped sealed bottle of drinking water (or distilled, whatever I’ve got) into a test tube or vial and then aspirate into a sterile syringe and use it from there.  It’s a shortcut that is not bulletproof and has potential to bite ya in the ass, but in my own experience, I’ve not yet encountered any contaminations over this past year.  That said, I don't make spore syringes or prepare stuff for others and am typically just hydrating spores for a bit before putting them to agar for cleanup (so, i’m expecting it to be dirty/non-sterile from the outset).  I’ve never bothered
to plate straight water using this approach…probably should though.


--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
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Re: Ask Quick Questions, Get Quick Answers [Re: flapping_bird] * 8
    #29077915 -



First Nat tub. Harvest now or let em ride a little longer?

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Re: Ask Quick Questions, Get Quick Answers [Re: Stuntbum]
    #29077918 -

nice tub-o-nats! Beautiful. Congrats!


--------------------
Peace to you, friend!
The HHG is a guide to getting started.
New User Welcome Wagon
GROWER’S BEST photo contest: join the fun!
CAKE WARS III - Starts SEPT 1, 2026

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: bluespirit13]
    #29077996 -

bluespirit13 said:
I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?


Did you have the needle pointing up in the PC? If not, try that.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: Stuntbum] * 2
    #29078039 -

Stuntbum said:


First Nat tub. Harvest now or let em ride a little longer?


I'd harvest that.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29078067 -

Shit dat wordt voor het eerst in 1000 jaar naar de Dirk


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No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: ThurmanMurman]
    #29078076 -

I think the water will boil and expand. And as such water will come out via the needle, right?
ThurmanMurman said:
bluespirit13 said:
I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?


Did you have the needle pointing up in the PC? If not, try that.


I think the water in the syringe will boil and expand. And as such water will come out via the needle, right? Regardless of it standing up.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: cybork] * 2
    #29078082 -

cybork said:
I think the water will boil and expand. And as such water will come out via the needle, right?
ThurmanMurman said:
bluespirit13 said:
I was under the impression you could sterilize a syringe of water by pressure cooking it wrapped in foil

My full syringe of water only had about 4cc of water left after pressure cooking it. What did I do wrong?


Did you have the needle pointing up in the PC? If not, try that.


I think the water in the syringe will boil and expand. And as such water will come out via the needle, right? Regardless of it standing up.


I've sterilized water in a needle several times. Put the cap on, point it so the needle is facing up wrap it in tin foil and it works. You lose a couple milliliters but not much.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: cybork]
    #29078084 -

Yes water will probably come out in every scenario, but by PCing the syringes with the neede upright only a little bit of water will boil over. If you PC them horizontally the syringe will be almost empty when you take it out of the PC. (At least this is what my personal experience has been with both options)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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