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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 7
    #28949506 -

Tres Soles



--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77] * 1
    #28949540 -

That’s impressive. Does the kit allow for a dilution when the results are at/close to the maximum indicated by the reference card?
Can you post a picture of the entire card? I’d like to see the values.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 1
    #28949552 -

Yeah I know some of the guys here only put in half the amount of mushroom sample with really potent stuff and then double the result. I guess it’s really hard to see when it gets that dark.


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LAGM2020

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a]
    #28949575 -

yes. might do that at a later point. dont want to use anothzr test atm.
here is the chart.



--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77]
    #28949590 -

Thanks chris.
I looked  up the kit and there is no problem with diluting. You’d be able to pull a mL out of the test vial, dump the remainder and add the mL back in and dilute with a mL of water to get a 50:50 dilution. Valid as long as it’s done within 4 hours.

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao]
    #28949627 -

The Tao said:
Thanks chris.
I looked  up the kit and there is no problem with diluting. You’d be able to pull a mL out of the test vial, dump the remainder and add the mL back in and dilute with a mL of water to get a 50:50 dilution. Valid as long as it’s done within 4 hours.


are you sure about this?
i just did that. kept 1 ml from the dark brown test liquid and diluted it with 1 ml tap water.

the result of this dilution would measure close to 18mg, maybe rather 17
x2 would equal 34mg ?


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: chris77]
    #28949662 -

The kit instructions had a section on dilution for extracts. The validation section said results need to be read within 4 hours to be valid.
Whenever I need to read a colorimetric test I like the test to be towards the middle of the card. I record the initial results then dilute and compare results.
You could pull up more than a mL of the test solution, pull the metal and septum off the vial, drain and tare the vial. Add a weighed mL of the test solution and a weighed mL of water back  to the vial to be accurate.

Edit: added a comma.

Edited by The Tao (09/17/24 12:39 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 3
    #28949680 -

The Tao said:
Sterilize or pasteurize? I’ve been pasteurizing using your dehydrator tek.


Sterilize. I run all my substrate bags through the PC just like I do my gourmets. Spawn in front of the flow hood, close bag, wait. I only pasteurize my casing at this point. This workflow is just easier for me at this point.

Happy to see that dehydrator tek working for people.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao]
    #28949701 -

The Tao said:
The kit instructions had a section on dilution for extracts. The validation section said results need to be read within 4 hours to be valid.
Whenever I need to read a colorimetric test I like the test to be towards the middle of the card. I record the initial results then dilute and compare results.
You could pull up more than a mL of the test solution, pull the metal and septum off the vial, drain and tare the vial. Add a weighed mL of the test solution and a weighed mL of water back  to the vial to be accurate.

Edit: added a comma.


ok wow, thanks!
well. it was my fiirst time using a testkit :smile:
thanks for clearing things up


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 1
    #28949856 -

The BRflF pucks are looking good in think



I was going them to make spawn or a mix of course and grain but I learned it's not recommended.

Then I have a transfer from water agar that after a couple of days looks like this



My intention was to make spawn with it but I don't like the gooey stuff. Do you think that's viable?

I'm thinking now of taking a bit of myc from the puck and make a plate, and then make spawn, if the former it's not viable.

Then make the pucks fruit, covering them with verm and putting them in a water chamber.

Does that make sense?


--------------------


Give away list

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 1
    #28949861 -

Yeah take another transfer from that puck and toss that other plate.  Those gooey things are bacterial colonies.  You can try to fruit what's left of the puck(s) later if you're inclined to.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: alaskappalachian]
    #28949892 -

Thanks! There's two tiny dots at 7 on clock that look like healthy mycelium.

Would it make sense to transfer them too?


--------------------


Give away list

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mush_monkey] * 1
    #28949898 -

Nah - you've got a wealth of genetic potential in a single transfer from any one of those clean(er) pucks.  Ditch that bugger.


--------------------

THE 49TH OYSTER BUCKET TEK

FREE WILL IS A SHADOW

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: alaskappalachian] * 24
    #28950901 -

Small spaghetti fruits? Sure. But yields!



:dancingshroom:


--------------------
:mushroom2::heart: Growing Semperviva/Subtropicalis - My standard procedure, it all comes down to the basics. :heart::mushroom2:



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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 1
    #28950998 -

Nice tub! Are you going to try to peel the entire harvest off in one piece?

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 4
    #28951153 -

Slow line spawned 8/21





Bag from the same LC knocked up on 8/12. Saving some for myceliated grain but might spawn half depending on how the tub turns out.





--------------------
LAGM2020

Edited by A.k.a (09/18/24 12:33 PM)

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: The Tao] * 1
    #28951285 -

The Tao said:
Nice tub! Are you going to try to peel the entire harvest off in one piece?


might be difficult not to take em off in one piece :wink:
great yield, Melgo
:highfive:


--------------------
the observer is the observed
            j. krishnamurti


>The Hitchhiker‘s Guide to the Shroomery<

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Melgo] * 1
    #28951371 -

Melgo said:
Small spaghetti fruits? Sure. But yields!



:dancingshroom:



Madness...


--------------------
Radio Gnome Invisible

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] * 7
    #28951389 -

Remember these guys?


That's them now. Feel old yet?



Spawned to ziploc bags. LC clone nocced jar 17.7.2023 (it was very veiny and had orange spots), spawned to coir in bags 21.7.2024, first big pins 14.8, harvested 17.9, not much, but I was happy for a quick pinning and pretty fast maturing comparing to older grows.

I have few small containers spawned from new SS jars and they're pinning almost at same rate, pins are beautiful, will report when they're a bit bigger


--------------------
Radio Gnome Invisible

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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri] * 5
    #28951526 -

Konbri said:
Melgo said:
Small spaghetti fruits? Sure. But yields!



:dancingshroom:



Madness...


Packed in tight!



--------------------
Low Prep the World! (agar plates)
Low Prep the World! (grain prep)
Trashcan FC
Acidulated extraction gummies and drops
Drying agar and grains using parchment paper
:leaf:Cleaning your bubble hash bags


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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