Mycolorado said:

Ah, ok….I misunderstood.  And good, as that wouldn’t make sense to me.  I’d just toss the problematic cultures and go back to spore unless those are super dope killer genetics you have to have.

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stockw said:

Thx for the suggestion

Found some funny dialogue between Josex & Bod lol

Sounds & looks like it's worth a try but I don't have a bunsen burner to hand so I suppose I'll just repurpose one of my monos with broken clips

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SirPsycho said:

You could do an open air transfer

Look up "bunsen burner sterile field"


Open air transfer from contaminated plate using this method.

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stockw said:

Doesn't look that weird to me? I've had bottles of agar look nearly identical & work fine. I have never forgotten to homogenise my MEA though..

Is the texture normal when you swirl it?

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SirPsycho said:

I use a "dab torch"

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Oh I've got one of those

You just leave it blasting above your plate & do the work yeah? Will try it out today. Cheers.

Arctic_Fox said:

It's solid, so I can't swirl it

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stockw said:

SirPsycho said:

I use a "dab torch"

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Oh I've got one of those

You just leave it blasting above your plate & do the work yeah? Will try it out today. Cheers.

Arctic_Fox said:

It's solid, so I can't swirl it

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I reckon heat it back up in boiling water give it a good mix & pour away. Colour's fine to me.

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pablotx said:

SirPsycho said:

You could do an open air transfer

Look up "bunsen burner sterile field"


Open air transfer from contaminated plate using this method.

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That's some old school stuff. I've got a (professional) microbiologist friend and this is all she uses in a commercial setting.

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I prefer to think of it as proven and time tested instead of old school

Scemilope said:

I finally put these cryos to work.

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