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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28403206 -

Arctic_Fox said:
Camera93 said:
That's why we streak in a pattern




And what do you use as a point of reference to remember where your streak pattern is?
I'm just asking because I don't usually see markings other than dates and varieties on other people's plates.



https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1

There's a gif of the streak patten at the end of OP


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28403210 -

Yeah but you have to mark something to remember which side of the circle is the top...
Never mind, it's not terribly important :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28403220 -

Do you guys just throw away old plates when they donโ€™t look great after taking a transfer or do you keep them for future use and if so, what for?

Ex: scalloped plates, non-uniform growth, thin/weak mycelium.

Being a noob Iโ€™ve got way too many of these and just tossing them feels a little painful but if thereโ€™s no use in keeping them after taking a transfer(s) I will:frown:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28403250 -

Arctic_Fox said:
Yeah but you have to mark something to remember which side of the circle is the top...
Never mind, it's not terribly important :lol:



The growth patten will make it very obvious. Did you see the pics of the inoculated plates under the gif?


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 4
    #28403279 -

Good thing I marked theseโ€ฆwouldnโ€™t have had a clue otherwise. :lol:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28403283 -

Mycolorado said:
Good thing I marked theseโ€ฆwouldnโ€™t have had a clue otherwise. :lol:




Looks moldy

:bathtub40lol:


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 1
    #28403313 -

:takingnotes:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28403341 -

I'm obviously referring to the growth stage that is days before this level of growth when the first germinations occur and it is unclear whether they are following the steak pattern. I assume the answer is that people with established pipelines inoculate so many plates that it doesn't matter. :shrug:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 3
    #28403348 -

Just giving you shit.  Youโ€™re overthinking the importance of where the streak was. Like sir said, and the example I posted, the germination is going to show up in the line/pattern you streak. If you follow the gif in c10โ€™s post, youโ€™re going to spread target spores and contaminants, both bacterial and fungal (should they be present), all around the plate and thatโ€™s goodโ€ฆin both cases, youโ€™re just trying to spread things out so you can select colonies of your target species to xfer from as opposed to having them all commingled together.


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๐Ÿ…‚๐Ÿ„ธ๐Ÿ„ถ๐Ÿ„ผ๐Ÿ„ฐ


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado] * 4
    #28403351 -

I love a good commingle

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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 1
    #28403353 -

:dogpile:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado] * 2
    #28403407 -

Good stuff guys. Haha ๐Ÿ˜† I love getting know you all and the personality behind each of the names and pictures.



Iโ€™m honored to know all of you and to share such a wonderful piece of the internet with you all.


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โ€œPeace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.โ€

Team Panaeolus

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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheAtlantean]
    #28403512 -



T1 from spore germ plate - ship it to grains?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nigel Stephens] * 2
    #28403521 -

Nigel Stephens said:


T1 from spore germ plate - ship it to grains?



Maybe do a transfer from 11 o'clock before taking this one to grain?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Throwaway]
    #28403571 -

Cool yeah will do.

What do you do with donor plates after transfers, let them pin and then clone?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nigel Stephens] * 1
    #28403593 -

Mostly toss them, but some people keep them. Depends on how much space you want to dedicate to plates


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A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28403701 -

I think this is good mycelium. Thoughts?



Same plate, different lighting.
(And I really need a new camera)


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Edited by Arctic_Fox (07/22/23 11:26 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28403714 -

Arctic_Fox said:
I think this is good mycelium. Thoughts?



Same plate, different lighting.
(And I really need a new camera)



Center spot yes. Iโ€™d transfer from the edge away from the adjacent spot after it grow some more

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28403724 -

MicroMycelium said:
Center spot yes. Iโ€™d transfer from the edge away from the adjacent spot after it grow some more



Thanks. Does something concern you about the other non center spot?

I inoculated this by placing a drop (just one drop) of spore solution on the surface of the agar. But since I didn't dig into the agar at all to make a pit for it to sit in, the spore solution rolled around the dish a bit (along with some condensation on the agar surface).


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox] * 1
    #28403730 -

Looks fine. Letโ€™m grow a bit to get a better read and make xfrs.


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๐Ÿ…‚๐Ÿ„ธ๐Ÿ„ถ๐Ÿ„ผ๐Ÿ„ฐ


Helpful Threads
stonesun
Mycoloradoโ€™s Tamp Fruit Project
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