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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28389052 -

nektar61 said:
Here's how I took the very clean prints:







This is how I like to do prints too, except with 10cm^2 foil squares for wrapping candies (or shroom chocolates) to avoid fumbling trying to cut foil nicely. Although, truth be told, I tend to print onto Petri dishes when I can't be bothered to set up a rack :wink:


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The fool who persists in his folly will become wise.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: phenyl] * 4
    #28389076 -

Loving this thread ❀️

So many plates and tipps to feed on :mushroom2:

Here's some of my latest results to share. Put all of it to grain and by now got tubs going for all of them apart from the B+ because I was still wary of those. Will post updates if there's contam or so. Had some intense metabolites on some of my PES Hawaiian grain that may well suggest a bacterial contamination (see this post and or last pic of grain)





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Re: AGAR ENVY! (Anything and All things agar!) [Re: tittlez]
    #28389157 -

tittlez said:
Loving this thread ❀️

So many plates and tipps to feed on :mushroom2:

Here's some of my latest results to share. Put all of it to grain and by now got tubs going for all of them apart from the B+ because I was still wary of those. Will post updates if there's contam or so. Had some intense metabolites on some of my PES Hawaiian grain that may well suggest a bacterial contamination (see this post and or last pic of grain)







:kimjongil:


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𝕴𝖋 𝕴 π–π–†π–›π–Š π–˜π–Šπ–Šπ–“ π–‹π–šπ–—π–™π–π–Šπ–— π–Žπ–™ π–Žπ–˜ π–‡π–ž π–˜π–™π–†π–“π–‰π–Žπ–“π–Œ π–šπ–•π–”π–“ π–™π–π–Š π–˜π–π–”π–šπ–‘π–‰π–Šπ–—π–˜ 𝖔𝖋 π•²π–Žπ–†π–“π–™π–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri] * 1
    #28389192 -

I just PC’ed my first No pour PP5 plastic cups and they warped a little on the bottom so the agar has a few thinner portions, they should still be good it use or should I redo the whole process?


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Edited by MicroMycelium (07/08/23 12:02 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28389200 -

Thinner and thicker agar can make the mycelium grow differently slightly. But as long as they're sterile they'll work fine.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28389489 -

Is caramelizing/overcooking sugars a myth?

Hi everyone
I see some of you warning not to PC the agar more than 20-30 mins.
The reason being that the sugars might caramelize or something.

Is this true or a myth? Has anyone confirmed this?

If it is so, is it valid for every nutrient source.

I'm thinking it might be valid for a simple sugar like maltose in MEA but not so much for complex starches like if we do a BRF yeast agar like the one Seeker uses.

For some reason I seem to be getting less contamination if I PC my no pours longer than 30 mins and I'm wondering if I can go an hour with all types of agar and not worry about fucking it up.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: drVetker] * 1
    #28389496 -

No PC as long as you want.


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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: drVetker] * 2
    #28389557 -

drVetker said:
Is caramelizing/overcooking sugars a myth?

Hi everyone
I see some of you warning not to PC the agar more than 20-30 mins.
The reason being that the sugars might caramelize or something.

Is this true or a myth? Has anyone confirmed this?


This is why I'm pedantic about shit, the noobs are too easily confused/lead astray.

Just google caramelization temps, you'll quickly discover it's a non issue. When in doubt, look under the profile avatar for the trusted cultivator tag. As mentioned by every TC posting in this thread, you can sterilize for longer than 20-30 mins.

Don't worry.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28389565 -

Stipe-n Cap said:
drVetker said:
Is caramelizing/overcooking sugars a myth?

Hi everyone
I see some of you warning not to PC the agar more than 20-30 mins.
The reason being that the sugars might caramelize or something.

Is this true or a myth? Has anyone confirmed this?


Thia is why I'm pedantic about shit, the noobs are too easily confused/lead astray.

Just google caramelization temps, you'll quickly discover it's a non issue. When in doubt, look under the profile avatar for thw trusted cultivator tag. As mentioned by every TC posting in this thread, you can sterilize for longer than 20-30 mins.

Don't worry.


Thank you sir

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 1
    #28389825 -

Stipe-n Cap said:
When in doubt, look under the profile avatar for the trusted cultivator tag.


drVetker, you can do searches here for answers only by TCs. Info on that, and saving a search to make it easy to reuse (including limiting to last 5 years for a lot of it) is here.

There are other users here who are solid who are not TCs, but you can't usually go wrong with a TC answer.

Picax, day 8 of spores sliced into agar, looking like happy caterpillars. I'm going to transfer T1 tonight. Will also keep these going, maybe send one to grain when full, certainly close back up and keep for plins to clone. These will be rhizo once transferred.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/09/23 01:23 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #28390051 -

nektar61 said:
Picax, day 8 of spores sliced into agar, looking like happy caterpillars. I'm going to transfer T1 tonight. Will also keep these going, maybe send one to grain when full, certainly close back up and keep for plins to clone. These will be rhizo once transferred.



Aw, yeah.  Very nice!


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β€œOne's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021

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Re: AGAR ENVY! (Anything and All things agar!) [Re: jay.ach]
    #28390168 -

heyo, im looking to get into agar and saw these dishes in a write up.
https://www.celltreat.com/products/standard-petri-dishes/
i saw that some people use films on their dishes, what for i have no idea, so would i need a "film" thingy for these dishes?


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PatrickKN:
"I would not twerk in a box. I would not twerk for a fox. I would twerk in a train. I would not twerk for Ukraine.

I do not like twerking 14 monkeys."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 14monkeys]
    #28390182 -

You might be thinking about parafilm, which is used to seal petris. You can use parafilm, cling wrap, or place them in a fresh ziplock bag.


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CV+

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MojaveMyc]
    #28390207 -

is it necessary or is it just for poured agar that you arent using yet?


--------------------
PatrickKN:
"I would not twerk in a box. I would not twerk for a fox. I would twerk in a train. I would not twerk for Ukraine.

I do not like twerking 14 monkeys."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 14monkeys] * 1
    #28390210 -

I keep unused plates in a ziplock.  Seal up after you use them.


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πŸ…‚πŸ„ΈπŸ„ΆπŸ„ΌπŸ„°


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28390215 -

ah ok thank you


--------------------
PatrickKN:
"I would not twerk in a box. I would not twerk for a fox. I would twerk in a train. I would not twerk for Ukraine.

I do not like twerking 14 monkeys."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 14monkeys]
    #28390309 -

This patchy unorganized growth outside the central mycelium just means it’s happy and it’s expanding everywhere since yesterday right? Right????



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πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 14monkeys]
    #28390406 -

14monkeys said:
heyo, im looking to get into agar and saw these dishes in a write up.
https://www.celltreat.com/products/standard-petri-dishes/
i saw that some people use films on their dishes, what for i have no idea, so would i need a "film" thingy for these dishes?


I, and some others here, use clear wide plant grafting tape, then tape it with micropore tape. It's cheap on eBay. Pic of plates like that right above your post, here:
https://www.shroomery.org/forums/showflat.php/Number/28389825#28389825

I used to use parafilm but this is much cheaper and easier.

Some agar info:
https://www.shroomery.org/forums/showflat.php/Number/27551422#27551422

Welcome.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/09/23 04:37 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 6
    #28390410 -

Wrapping in grafting tape and then with micropore sounds like a clusterfuck.


--------------------
πŸ…‚πŸ„ΈπŸ„ΆπŸ„ΌπŸ„°


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28390413 -

Mycolorado said:
Wrapping in grafting tape and then with micropore sounds like a clusterfuck.


It's not. It's really quick. I use about 3 turns around with grafting and 1 turn around with micropore.


Curious, what do you use?


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras: Filter Print Post Top
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Shop: MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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