Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Cultivation Supplies   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: < First | | 1051 | 1052 | 1053 | 1054 | 1055 | 1056 | 1057 | 1058 | 1059 | 1060 | 1061 | 1062 | 1063 | 1064 | 1065 | 1066 | 1067 | 1068 | 1069 | 1070 | 1071 | | Last >
Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 1
    #28325143 -

sprocket said:
fiddle_head said:
I think the cost of diminishing returns with the slurry is medium/medium-high. The difference may be observable, but I don't think it will be significant enough to warrant a second attempt. Let us know what you find out though if you do attempt for sure, be interested to know.


I just did 8 traditional transfers today, but still may give the slurry idea a go. If I do, I'll absolutely report my findings back here good or bad. Worst case scenario, I'll use the remaining slurry to inoculated some grains or a brf cake.  Have a great day!


Keep it real sprocket ✌️


--------------------
----<><><>----
⁂ tail-lights, back-pockets, rearview mirrors ⁂

⁂ guides | grows | supreme air box | sporeaddict ⁂

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 1
    #28325648 -

sprocket said:
Has anyone done this? I want to do a bunch of transfers for inoculating grain jars with agar. My thought was taking my donor cup and adding an ml or two of sterile water to make an agar slurry. Then take an inoculation loop, dip it into the slurry and inoculate the other cups that way. Seems a lot quicker and easier than cutting samples out with a scalpel.  Any thoughts on this?


I think I'm going to try this except use a small syringe to suck up the slurry and then put several drops on agar.  Should be fast and easy. Thanks for the idea.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 6
    #28325713 -


Lion's Mane LC tester

I should really throw all my glass petris in some vinegar to get rid of all the stains next time I cook up a batch


--------------------
How I grow mushrooms 🍄
How I do agar 🦠

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Throwaway] * 2
    #28325884 -

When I poured 40 agar cups, I thought to myself, this will last me a while....4 days later half of them are inoculated.  Looks like I'm going to have to pour some more soon, what a shame.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket]
    #28325940 -

Can I get some feedback on if anything here is worth transfering?



Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bjjrev] * 4
    #28325943 -

bjjrev said:
Can I get some feedback on if anything here is worth transfering?





Third pic, nice round colony at two o’clock looks really nice.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns]
    #28325961 -

3 triangles out of that? And put mycelium down on 3 clean plates?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bjjrev] * 1
    #28325976 -

I would be tempted to grab a couple transfers from the plate 4 as well @ 5 & 10 oclock from the swab growth.  Plate 1 - the culture on the left has usable growth @ 5oclock as well.  Having more starting cultures will give you greater variety in the phenos that result - handy with nats since some cultures have overlay/growth issues.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns]
    #28325992 -

RoscoeReturns said:
bjjrev said:
Can I get some feedback on if anything here is worth transfering?





Third pic, nice round colony at two o’clock looks really nice.


:whathesaid:


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy] * 2
    #28325999 -

Like this?


Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bjjrev] * 1
    #28326004 -

Yeah, I'd personally go with the selections in pic two.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy] * 1
    #28326016 -

Mr Piggy said:
Yeah, I'd personally go with the selections in pic two.


This is my first attempt... I cut the triangles with a sterilized blade and put them in a new plate mycelium facing the agar correct?

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: bjjrev] * 1
    #28326020 -

It doesn't matter which direction the myc faces, as long as it's quickly nestled in the center of the plate.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy]
    #28326235 -

This is Pan. Jamaica. I see these little white specs almost like pins. They are under what looks like a fine layer of myc. Is this good to go? Sorry for the shitty picture.



--------------------
Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum





Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] * 1
    #28327055 -

Well, I used to me a member of this forum 20 years ago when I first cultivated GTs.  I had to stop because it was dumb for me to do it while active duty military.  I retired 5 months ago and am back at it.  Flow Hood and more equipment will arrive next week.  Agar has been inoculated.  Shopping around for a autoclave now.


Edited by crash_compton (05/20/23 01:39 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: crash_compton] * 1
    #28327063 -

Nice.:thumbup:

What's the lighter for? I hope you are not using that to flame sterilize shit inside the SAB.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

Edited by altford78 (05/20/23 02:05 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: crash_compton] * 1
    #28327240 -

crash_compton said:
Well, I used to me a member of this forum 20 years ago when I first cultivated GTs.  I had to stop because it was dumb for me to do it while active duty military.  I retired 5 months ago and am back at it.  Flow Hood and more equipment will arrive next week.  Agar has been inoculated.  Shopping around for a autoclave now.




First and foremost thank you for your years of service!

To touch on the lighter. You want to flame sterilize you tools on the outside of the SAB. The flame can cause turbulent air movement and kick the nastiness back up in the air. Also if your using isopropyl alcohol in you box it’s a flame hazard. Welcome back!!!


--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 2
    #28327245 -

I was flaming out of the SAB but just took the lighter in and out of the sab with my hand so i did not have to reach around the table every time.  Also did use iso in the SAB a little before opening the bags i had syringes in.  First time using a sab.  Back in the day i just made a clean room in the house.

Edited by crash_compton (05/20/23 08:20 AM)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: crash_compton] * 3
    #28327304 -

crash_compton said:
I was flaming out of the SAB but just took the lighter in and out of the sab with my hand so i did not have to reach around the table every time.  Also did use iso in the SAB a little before opening the bags i had syringes in.  First time using a sab.  Back in the day i just made a clean room in the house.


You'll be happier with a small torch than a bic.


--------------------
Mold cultivator extraordinaire

I also dabble in bacteria

I'm also a retard sometimes

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: altford78]
    #28327355 -

altford78 said:
crash_compton said:
I was flaming out of the SAB but just took the lighter in and out of the sab with my hand so i did not have to reach around the table every time.  Also did use iso in the SAB a little before opening the bags i had syringes in.  First time using a sab.  Back in the day i just made a clean room in the house.


You'll be happier with a small torch than a bic.


I have a alcohol lamp and plenty of torches.  This is what I chose to use today.  I didnt feel like grabbing my torch in the shop while I was doing this because I was watching the WHL championship game. I am in the process of moving so my shits all over.  Thanks for the tip.

Edited by crash_compton (05/20/23 09:24 AM)

Extras: Filter Print Post Top
Jump to top Pages: < First | | 1051 | 1052 | 1053 | 1054 | 1055 | 1056 | 1057 | 1058 | 1059 | 1060 | 1061 | 1062 | 1063 | 1064 | 1065 | 1066 | 1067 | 1068 | 1069 | 1070 | 1071 | | Last >

Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   North Spore Cultivation Supplies   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 3,829 6 03/31/06 05:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 8,363 8 02/14/05 07:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,996 8 02/25/05 11:03 AM
by agar
* test tubes agar fungophiliac 3,689 8 06/19/20 09:41 PM
by gorilliam
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 5,320 12 03/30/20 10:37 AM
by Surf707
* Agar and the global economy Dem_Bones 1,930 4 03/01/06 09:08 AM
by Dem_Bones
* Agar question WormholeSurfer 1,688 3 10/29/05 07:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )		 agar 22,278 75 02/12/21 05:50 AM
by Bart84

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
765,895 topic views. 25 members, 821 guests and 250 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.054 seconds spending 0.027 seconds on 18 queries.