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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yuggoth] * 2
    #28322560 -

Yuggoth said:



What do we think is going on here? I see the hose hooked up to it. Warm empty tank being filled incorrectly with cold liquid?


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] * 6
    #28322562 -

Hey boss, it was like that when I got here!


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We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

Things I really wish I knew when I started // Vacuum sealer discussion thread // Shroomery gif zoo

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28322716 -



Love it when I get a clean germ plate, giant penis envy


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head] * 1
    #28322731 -

I've got super thick mycelial growth on all my t4 transfer plates currently running, they're 13 days in. Is this normal or indicative of stress? Bad pics soz but my camera's not the best. They're vertically fat also. What are ye thoughts!


Edited by stockw (05/17/23 03:34 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28322734 -

Does this plate look ok or am I looking at some sort of contam?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw]
    #28322762 -

stockw said:
I've got super thick mycelial growth on all my t4 transfer plates currently running, they're 13 days in. Is this normal or indicative of stress? Bad pics soz but my camera's not the best. They're vertically fat also. What are ye thoughts!




Agar recipe?


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How I grow mushrooms 🍄
How I do agar 🦠

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Throwaway] * 1
    #28322766 -

It's a premix I've had for ages but it's Malt Extract, Dextrose & Agar

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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw] * 1
    #28322770 -

stockw said:
I've got super thick mycelial growth on all my t4 transfer plates currently running, they're 13 days in. Is this normal or indicative of stress? Bad pics soz but my camera's not the best. They're vertically fat also. What are ye thoughts!




Looks like healthy tomentose mycelium. I’ve seen that puffy halo on the outer circumference on certain varieties before, I don’t think it’s anything to worry about. What variety is it?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: stockw] * 1
    #28322772 -

stockw said:
It's a premix I've had for ages but it's Malt Extract, Dextrose & Agar


The amounts matter. I'd say this is pretty normal looking growth for a higher nute agar. If this plate barely has any nutes I'd be worried. Since it's premix which probably wont be low nute I think this looks pretty good.


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How I grow mushrooms 🍄
How I do agar 🦠

Edited by Throwaway (05/17/23 04:50 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Throwaway]
    #28322779 -

Looks like healthy tomentose mycelium. I’ve seen that puffy halo on the outer circumference on certain varieties before, I don’t think it’s anything to worry about. What variety is it?


Interesting because the previous plate was fully rhizo. It's z-strain (:

Throwaway said:
stockw said:
It's a premix I've had for ages but it's Malt Extract, Dextrose & Agar


The amounts matter. I'd say this is pretty normal looking growth for a higher nute agar. If this plate barely has any nutes I'd be worried. Since it's premix which probably wont be low nute I think this looks pretty good.


Great to hear, cheers guys. I'm binning off LC's & going full a2g / g2g with these plates so glad they're doing well.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28323874 -

SirPsycho said:
Ummm.... If you're sterilizing agar in a media bottle in the pc there is no reason to pre-boil. Just throw everything in the bottle and run the pc. No need to boil it so can you boil it.
[...]


Umm... just throw the powders and water in the bottle and PC? it will dissolve correctly without agitation?

That seems to be what you're implying, and if that's the case, boy, I feel dumb.


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No responsibility do we owe greater than that to ourselves to become the greatest to which our potential calls us.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seamus]
    #28323880 -

i put cold water in the bottle. add dry ingredients. shake and pc 20min.

when i put the bottle in the fridge overnight with just nutes adding agar the morning after and then pc?  the agar comes out clear as glass if using lme.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Edited by mushboy (05/17/23 07:41 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seamus]
    #28323881 -

seamus said:
SirPsycho said:
Ummm.... If you're sterilizing agar in a media bottle in the pc there is no reason to pre-boil. Just throw everything in the bottle and run the pc. No need to boil it so can you boil it.
[...]


Umm... just throw the powders and water in the bottle and PC? it will dissolve correctly without agitation?

That seems to be what you're implying, and if that's the case, boy, I feel dumb.


I shake it thoroughly beforehand but yea it will


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Re: AGAR ENVY! (Anything and All things agar!) [Re: seamus]
    #28323885 -

Correct


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Yeah, yeah.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NFLProof]
    #28323958 -

Has anyone done this? I want to do a bunch of transfers for inoculating grain jars with agar. My thought was taking my donor cup and adding an ml or two of sterile water to make an agar slurry. Then take an inoculation loop, dip it into the slurry and inoculate the other cups that way. Seems a lot quicker and easier than cutting samples out with a scalpel.  Any thoughts on this?


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How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sprocket] * 1
    #28324030 -

sprocket said:
Has anyone done this? I want to do a bunch of transfers for inoculating grain jars with agar. My thought was taking my donor cup and adding an ml or two of sterile water to make an agar slurry. Then take an inoculation loop, dip it into the slurry and inoculate the other cups that way. Seems a lot quicker and easier than cutting samples out with a scalpel.  Any thoughts on this?


I just scrape hyphae from the surface of the agar or do an aerial transfer with an inoc loop, no need to make a slurry


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28324270 -



These older ATL7 plates are starting to look mean

Edit: here's the bottom plate's LC on grain because I'm so excited. Inoculated on 23-04



:rush:

Edited by Throwaway (05/18/23 02:23 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head]
    #28324296 -

fiddle_head said:
sprocket said:
Has anyone done this? I want to do a bunch of transfers for inoculating grain jars with agar. My thought was taking my donor cup and adding an ml or two of sterile water to make an agar slurry. Then take an inoculation loop, dip it into the slurry and inoculate the other cups that way. Seems a lot quicker and easier than cutting samples out with a scalpel.  Any thoughts on this?


I just scrape hyphae from the surface of the agar or do an aerial transfer with an inoc loop, no need to make a slurry


Sounds even easier. My reasoning behind the slurry was to use a 5 or 10ul loop then transfer and streak the slurry a little to get more/faster colonization.


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28325044 -

I think the cost of diminishing returns with the slurry is medium/medium-high. The difference may be observable, but I don't think it will be significant enough to warrant a second attempt. Let us know what you find out though if you do attempt for sure, be interested to know.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head] * 1
    #28325141 -

fiddle_head said:
I think the cost of diminishing returns with the slurry is medium/medium-high. The difference may be observable, but I don't think it will be significant enough to warrant a second attempt. Let us know what you find out though if you do attempt for sure, be interested to know.


I just did 8 traditional transfers today, but still may give the slurry idea a go. If I do, I'll absolutely report my findings back here good or bad. Worst case scenario, I'll use the remaining slurry to inoculated some grains or a brf cake.  Have a great day!


--------------------
How do pirates clone their mushrooms?

        With agarrrrrr.:captain::captain:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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