Eugene Gesuale said:

What the fuck is going on with all these exotic species now!

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nektar61 said:

Request from user  x7x_x7x. F4 x7x tall pheno germ plates growing out to get plins to clone (to branch off to another line, clones, not spores):

^(The light blue line is a reflection of a string of LED lights, notice it's in a different place on the second photo)



F5 x7x tall pheno T1, 3 days on washed, soaked, PCed bird seed:


F4 x7x tall pheno T1 plates I'm not using, grown on modified Water Agar (with everything except dextrose). I'm going to put in cold storage in case I have to go back to this gen. These ones were not growing fast enough and were too weird to send to grain. I already sent the better ones.

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x7x_x7x said:

mine grow patched too. amazing cultures in the last pics!

what about water agar? never heard that before.

thanks!

x7x_x7x

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Canna-bliss1 said:

Riptide lookin' wild

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At what point do we think we have a 'good isolate' when having plates like that? Can you take transfers of that to many plates and call those plates a good isolate to use for innoculating?







Took a couple transfers of this once I saw the insane rhizo growth a few days ago. It just continues on like a freight train.

Weioo said:

Took a couple transfers of this once I saw the insane rhizo growth a few days ago. It just continues on like a freight train.

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I would say your mycelium is full of vigor. Seems this specimen has something to prove...I like it.

Weioo said:

Canna-bliss1 said:

Riptide lookin' wild

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At what point do we think we have a 'good isolate' when having plates like that? Can you take transfers of that to many plates and call those plates a good isolate to use for innoculating?







Took a couple transfers of this once I saw the insane rhizo growth a few days ago. It just continues on like a freight train.

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I have a handful of transfers to go, only at T2. I'm working to get at least 4 plates that are near iso and hope one of em is a gem👍 Riptide is the only strain I'm working with to have that crazy lanky rhyzo on all the plates.

Weioo said:

So the general consensus is to take agar to T4 plates, gotcha. I'm as far as T2 on some of mine and they already look good but I'll continue on. They're from a swab but the plates don't like to show much sectioning.

Any reason to let your plates grow out for so long before transfering again? I can't see any sectioning with my noob eye, lol.

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Those Riptide transfers ripped across the plate before I could get to them. I usually let them get to about this point before I make any decisions. Here's a pan cyan plate about to be worked with tonight.

Weioo said:

So the general consensus is to take agar to T4 plates, gotcha. I'm as far as T2 on some of mine and they already look good but I'll continue on. They're from a swab but the plates don't like to show much sectioning.

Any reason to let your plates grow out for so long before transfering again? I can't see any sectioning with my noob eye, lol.

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Weioo said:

Thank you so much, that's super helpful! Except, could you please elaborate on 'fastest clowns you can get?' lol. Are you saying said pins to grain is a super fast colonizer?

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nogoodnamesleft said:

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Horsewithnoname said:

Quick agar noob question: I sometimes see T1, T2, T3, etc for talking about agar. I assume we're talking about the transfer count since the first agar plate was inoculated, right?

I also have seen some F1/F2/F3/etc sometimes. What do those stand for?

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stubb said:

Horsewithnoname said:

Quick agar noob question: I sometimes see T1, T2, T3, etc for talking about agar. I assume we're talking about the transfer count since the first agar plate was inoculated, right?

I also have seen some F1/F2/F3/etc sometimes. What do those stand for?

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'Filial', it denotes generation count.  So, starting from grandpa makes my dad F1, which makes me F2.

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