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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 2
    #28154181 -

Guerrilla said:
No. Clones are taken from inner tissue.


I realize that but you're still working near surfaces that were exposed to open air. I just would not ever trust that to be 100% clean. While the risk is very low if your technique is good I would still do atleast one transfer to be safe.


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P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NuclearTidalWave] * 4
    #28154197 -

Sorry to double post but here is a Tidalwave plin I'm quite proud of. I know it aint much compared to what I see posted here but it's my favorite plate yet getting back into things after taking a several year break from the hobby.



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P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NuclearTidalWave] * 1
    #28154201 -

NuclearTidalWave said:
I realize that but you're still working near surfaces that were exposed to open air. I just would not ever trust that to be 100% clean.


.......what? :rofl:

Dude, with that logic we wouldn't ever get anything clean.

We expose sterile, inner tissue, in a clean / sterile environment, using sterile technique.

You're massively overthinking it.

A large majority here that clone never find T1 necessary for a clean culture.

This is why many go to straight to grain with tissue, and succeed.


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Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 1
    #28154211 -

Guerrilla said:
NuclearTidalWave said:
I realize that but you're still working near surfaces that were exposed to open air. I just would not ever trust that to be 100% clean.


.......what? :rofl:

Dude, with that logic we wouldn't ever get anything clean.

We expose sterile, inner tissue, in a clean / sterile environment, using sterile technique.

You're massively overthinking it.

A large majority here that clone never find T1 necessary for a clean culture.

This is why many go to straight to grain with tissue, and succeed.


I see your point and realize I've definitely been overthinking the situation. Like I said I've only done one clone at this point. Good to know.


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Signature text goes here

P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NuclearTidalWave] * 5
    #28154218 -

Sorry dude I didn't mean to sound like a dick there, I realise that I did.

<3


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Being pissed on does not make you a real man.

...OR DOES IT?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28154220 -

Once you clone a few times it becomes apparent that inner tissue is safe.

After some practice it’ll click 👌


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 3
    #28154228 -

Guerrilla said:
Sorry dude I didn't mean to sound like a dick there, I realise that I did.

<3


Lol you'd have to come at me a lot harder than that to offend me. You taught me something and I appreciate that.


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Signature text goes here

P̫̻̳̮͎̥̬͋͊̂͐Ä̡͓̜̩̤̠̮́́̔̽̇Ṛ̹̱ͅA̠͉͒F̹̦̣͇̘̻̍̎ͬ͋͒̅͌͘I̖̻ͨͧ̇̋ͯ͡L̩̩͋́M͕̳͎̱̎ ̤̜͇̰̉̿ͫ̑͑͡ͅC̶̲̝̉̔U͚̙͕̬̘̗̐ͮ̀ͣ̎L̏́̂͋Ṯ̥̪̠ͩ͛̃͊
.

Edited by NuclearTidalWave (01/23/23 07:05 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NuclearTidalWave] * 1
    #28154257 -

Made up several plates. Attempted to inoculate 8 dishes with MSS. You can even see the spores floating on top several. It has been 2 weeks and not a single dish has done anything. No mycelium OR contam. Somehow I doubt my sterile technique is so stellar. After doing some MSS to grain, I Went G2A on these same plates and they took off very well. What gives? Any ideas?





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Edited by AspectOfTheCreator (01/23/23 07:10 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28154280 -

Your agar could be too firm. Overly firm agar can sometimes stop spores from germinating.

Also, did you use a loop or just squirt some on there?


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 7
    #28154663 -

Some ghost looking beautiful


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LAGM 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 1
    #28154672 -

Guerrilla said:
NuclearTidalWave said:
I realize that but you're still working near surfaces that were exposed to open air. I just would not ever trust that to be 100% clean.


.......what? :rofl:

Dude, with that logic we wouldn't ever get anything clean.

We expose sterile, inner tissue, in a clean / sterile environment, using sterile technique.

You're massively overthinking it.

A large majority here that clone never find T1 necessary for a clean culture.

This is why many go to straight to grain with tissue, and succeed.


I have a similar mindset as Nuclear on this. Just yesterday I did inoculate a couple bags with T1 clones from the interior of fruits so I am waiting to see what happens but I got to say I only did so because the plates looked so happy.

I will update when I see the results but my gut is always telling me to make "just 1 more transfer".

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NuclearTidalWave]
    #28154680 -

NuclearTidalWave said:
I see your point and realize I've definitely been overthinking the situation. Like I said I've only done one clone at this point. Good to know.


I just took my first clone to agar as well.

IIUC, cloning will (hopefully) alleviate the multiple dehydratioin cycles I currently have to go through with each tub - as well as yield fruit that is more uniform.

My plan is to then take grains that are spawned to new jars while the rest gets fruited.

CocaineBuffet said:
I have a similar mindset as Nuclear on this. Just yesterday I did inoculate a couple bags with T1 clones from the interior of fruits so I am waiting to see what happens but I got to say I only did so because the plates looked so happy.

I will update when I see the results but my gut is always telling me to make "just 1 more transfer".


It will be interesting to see whether you get uniform fruits and whether they grow at a similar rate.


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🅃🄴🄰🄼  🄲🄻🄸🄽🄶🅆🅁🄰🄿

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobgas]
    #28154750 -

Re cloning, do you always take tissue from the stipe or is it ok to take it from the cap? I've only done a couple of clones so far but was wondering about this a couple days ago.

Edited by Bajazly (01/24/23 12:27 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bajazly] * 2
    #28154768 -

Anywhere you can get some clean internal tissue is fine.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bajazly]
    #28154776 -

Bajazly said:
Re cloning, do you always take tissue from the stipe or is it ok to take it from the cap? I've only done a couple of clones so far but was wondering about this a couple days ago.


If you filled a 5 gallon bucket with agar and split a whole mushroom in half then placed it on top of the bucket you'd see mycelium growing from both the stipe and cap :lol:...lol

:deathstar:


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Mushroom Cultivation Techniques...

Edited by Grogu (01/24/23 01:27 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #28154795 -

Why does this example include a 5 gallon bucket of agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cooleko] * 5
    #28154797 -

Makes perfect sense to me... Not sure how you'd do it without the bucket....


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Question everything, it's very responsive.

Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28154798 -

Bucket tek


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28154803 -

I gotta buy another damn bucket?


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy] * 3
    #28154806 -

Yes.

Then ruin it with some holes just so you can decide you didn't really need the holes and then tape them over with monkey tape.


--------------------
Question everything, it's very responsive.

Culture Storage

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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