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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 4
    #28042792 -



T1 Ghost


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I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28042805 -

Trying to get this Blue Meanie cleaned up.  Is this even myc?  And suggestions on how to proceed? 



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rabbit84]
    #28042808 -

Rabbit84 said:
Trying to get this Blue Meanie cleaned up.  Is this even myc?  And suggestions on how to proceed? 




Yes, it is mycelium just not the mycelium you're looking for.

You should start over from spore


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28042810 -

SirPsycho said:
Rabbit84 said:
Trying to get this Blue Meanie cleaned up.  Is this even myc?  And suggestions on how to proceed? 




Yes, it is mycelium just not the mycelium you're looking for.

You should start over from spore


Is it a random mycillium contam then?  And yeah, I'll start over on that one.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28042812 -

Yeah that be mold (mold is also myc - that's what SirP was saying)


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rabbit84]
    #28042814 -

Rabbit84 said:
SirPsycho said:
Rabbit84 said:
Trying to get this Blue Meanie cleaned up.  Is this even myc?  And suggestions on how to proceed? 




Yes, it is mycelium just not the mycelium you're looking for.

You should start over from spore


Is it a random mycillium contam then?  And yeah, I'll start over on that one.


Yes, that is the mycelium of some kind of mold.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096]
    #28042835 -

Rusty2096 said:
Yeah that be mold (mold is also myc - that's what SirP was saying)


Well I just learned something, lol.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rabbit84]
    #28042842 -

Rabbit84 said:
Rusty2096 said:
Yeah that be mold (mold is also myc - that's what SirP was saying)


Well I just learned something, lol.


All fungi produce mycelium. I guess more accurately they are mycelium


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28042843 -

SirPsycho said:
Rabbit84 said:
Rusty2096 said:
Yeah that be mold (mold is also myc - that's what SirP was saying)


Well I just learned something, lol.


All fungi produce mycelium. I guess more accurately they are mycelium


Fungi


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096] * 1
    #28042847 -

Rusty2096 said:
SirPsycho said:
Rabbit84 said:
Rusty2096 said:
Yeah that be mold (mold is also myc - that's what SirP was saying)


Well I just learned something, lol.


All fungi produce mycelium. I guess more accurately they are mycelium


Fungi


Right, yeast doesn't produce mycelium


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28042895 -

alright, so that raises a question my pea brain hasn't been able to reason about.

What's the deal with Nutritional Yeast in agar? Yeast is yeast, a competitive organism to what we want, which is our choice of fungal culture, be that what it may. So why add yeast to the mix and potentially introduce sterilization process resistant microbes to a mix that we hope to be sterile?

Full disclosure, I've done zero googling on if yeast can survive PC temps nor its competitiveness towards mushroom mycelium.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: thehiveatp] * 3
    #28042910 -

thehiveatp said:
alright, so that raises a question my pea brain hasn't been able to reason about.

What's the deal with Nutritional Yeast in agar? Yeast is yeast, a competitive organism to what we want, which is our choice of fungal culture, be that what it may. So why add yeast to the mix and potentially introduce sterilization process resistant microbes to a mix that we hope to be sterile?

Full disclosure, I've done zero googling on if yeast can survive PC temps nor its competitiveness towards mushroom mycelium.



Nutritional yeast is dead long before we ever get it. Fuck, they sell cheese flavored nutritional yeast that people sprinkle on their popcorn :shrug:

The PC *sterilizes* agar anyway. Even if it were live yeast going in, it wouldn't be coming out. the nutritional yeast adds extra nutrients for the mycelium. I'll never go back to not using it


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 1
    #28042911 -

I use any type of yeast I have. Sometimes I use active dry yeast for bread. It all dies in the pc. :whathesaid:


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Question everything, it's very responsive.

Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #28042919 -

appreciate the lack of flame, but my underlying question is why add it to the agar?

what specifically is it offering to the mycelium, or is adding it just a hold over from the clandestine "ol Hippie3 did it, said it worked, so i just keep on doin' it since nothin gone wrong" TEK days?

Nichrome said:
I use any type of yeast I have. Sometimes I use active dry yeast for bread. It all dies in the pc. :whathesaid:



completely missed this.. pretty sure you need to use active dry yeast for bread unless you're making heavy bread or sour dough, which you still can make your own yeast cultures for. you makin a funny at me? :drysmash:

Edited by thehiveatp (11/10/22 12:01 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: thehiveatp] * 2
    #28042923 -

Proteins and a myriad of other nutrients otherwise unavailable to the mycelium with DME or GSA alone. It's not old hippie talk. I'm actually pretty confident it's fairly new to the agar scene


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 1
    #28042927 -

Beta glucan is your friend.


--------------------
Question everything, it's very responsive.

Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: thehiveatp]
    #28042932 -

Quote:
thehiveatp said:completely missed this.. pretty sure you need to use active dry yeast for bread unless you're making heavy bread or sour dough, which you still can make your own yeast cultures for. you makin a funny at me? :drysmash:



Completely missed the point of this....


--------------------



I :heart: 5318008


NOT a virgin!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #28042933 -

bigfootscreepyuncl said:
Proteins and a myriad of other nutrients otherwise unavailable to the mycelium with DME or GSA alone. It's not old hippie talk. I'm actually pretty confident it's fairly new to the agar scene


ah ok, that's makes sense. i'm quickly becoming much more curious about microbiology in general, and am trying to understand the purpose of each additive.

for instance, LME is for nutrients for a whole myriad of fungi and bacteria. Dyes can be used for a variety of reasons, though one must be mindful depending on the cultures they're looking to work with.

i've come across a few whitepapers in the past 24 hours regarding the use of charcoal, but haven't fully grokked anything substantial yet. maybe it's a throwback to my 2000s era goth days, but those black plates look dark and mysterious, even if the charcoal doesn't do anything significant.

sorry, a little tipsy here. so, long story short, i'm quickly becoming very curious about microbiology, specifically bacterias. it'd be super cool to have some kind of guide, similar to a mushroom field guide, for hobby mycologists that shows cultures of various bacterias (like, I'd like to know wtf that mucus-y yellow shit that's hit a few of my plates is).

even further I'm really curious how the hell pediatricians shoved a swap down my throat and were able to confirm i had strep in a super non-sterile environment. agar has opened a brave new world to me, and i'm diving in.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: thehiveatp]
    #28043030 -

This thread has a lot of pictures of contaminates in it. https://www.shroomery.org/forums/showflat.php/Number/17231150#17231150

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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #28043041 -

Damn that's pretty


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bodhisatta said:
figure it out listen to the cake

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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