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 YSr
Stranger
Registered: 04/13/04
Posts: 81
Last seen: 10 years, 11 months
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 Rhizomorphic growth on agar 
#3826052 - 02/24/05 04:01 AM (12 years, 3 months ago) |
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What kind of agar receipes gives good rhizomorphic growth on agar?
YSr
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 6 years, 2 months
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Re: Rhizomorphic growth on agar [Re: YSr]
#3826118 - 02/24/05 05:18 AM (12 years, 3 months ago) |
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Malt Extract Agar
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http://www.bruceeisner.com/ -Creating a Sensible Culture
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 6 years, 2 months
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Re: Rhizomorphic growth on agar [Re: liveby]
#3826132 - 02/24/05 05:27 AM (12 years, 3 months ago) |
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--------------------
http://www.bruceeisner.com/ -Creating a Sensible Culture
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 tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: Rhizomorphic growth on agar [Re: YSr]
#3826472 - 02/24/05 10:02 AM (12 years, 3 months ago) |
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There are many , the important thing to remeber , especially if going to make your own from scratch is PH levels. Rhizo growth is strain related in most cases. Cubes are known and set the example of "typicaly" rhizomorphic , where as Cyans tend to be more cottony in nature. The next key to learnig agar work and strain issolation is "sectoring" . You want to issolate a strain that does not sector(having both types of mycelial growth) and has potential to fruit well. There is more to it that I have mentioned but that is the basics so to say.
I for one like using wheat based MAE. PDA is okay , but I have found that a couple of strains tend to get real whispy and arieal when using this agar mix.
There is a recipe for oatmeal based agar that would probably do real well also.
GL
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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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YSr
Stranger
Registered: 04/13/04
Posts: 81
Last seen: 10 years, 11 months
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Re: Rhizomorphic growth on agar [Re: tripndicular]
#3832443 - 02/25/05 12:34 PM (12 years, 3 months ago) |
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Thanks you answering...
You say PH levels is very important. What is the "right" PH for Cubensis agar then?
YSr
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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Re: Rhizomorphic growth on agar [Re: YSr]
#3832663 - 02/25/05 01:33 PM (12 years, 3 months ago) |
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I'd guess its the same as the substrate, 7.5 being ideal. 6.5-7 should work ok though. I'd think the agar would be neutral but I guess it would depend on your water..
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Rhizomorphic growth on agar [Re: YSr]
#3833083 - 02/25/05 02:47 PM (12 years, 3 months ago) |
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Quote:
YSr said:
What kind of agar receipes gives good rhizomorphic growth on agar?
YSr
I like using MEA and PDYA the most.
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with  on there names, that means they have mushrooms for sale.
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tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: Rhizomorphic growth on agar [Re: YSr]
#3833118 - 02/25/05 02:53 PM (12 years, 3 months ago) |
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Quote:
YSr said:
Thanks you answering...
You say PH levels is very important. What is the "right" PH for Cubensis agar then?
YSr
Neutral on the PH or 7.0 you can sway around in rnges like scatman mentioned.
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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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YSr
Stranger
Registered: 04/13/04
Posts: 81
Last seen: 10 years, 11 months
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Re: Rhizomorphic growth on agar [Re: Roadkill]
#3835852 - 02/26/05 06:42 AM (12 years, 3 months ago) |
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Thanks again!
How much ME, yeast and agar do you use per liter? If adding peptone, how much?
YSr
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Anno
Experimenter
Registered: 06/18/99
Posts: 24,123
Loc: my room
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Re: Rhizomorphic growth on agar [Re: YSr]
#3835985 - 02/26/05 08:59 AM (12 years, 3 months ago) |
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Quote:
liveby said:
sorry for being rude ! link http://www.shroomery.org/index/par/23367
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Rhizomorphic growth on agar [Re: YSr]
#3835992 - 02/26/05 09:06 AM (12 years, 3 months ago) |
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YW
I follow pretty much the same recipies as are stated in the FAQ.
Copy and Pasted from the FAQ :
Quote:
These recipes are designed for agar maintenance of Psilocybe species. All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure. 500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.
Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)
If you read the PDYA one there are several different ways you can make it.
This is the one I make.
-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast
I switch back and forth with the above PDYA recipy and the MEA.
And have always gotten great results.
Click here for all the agar recipies that are listed!~
good luck!~
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.
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