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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW]
    #27932862 -

HILLBILLY OUTLAW said:
:super:


Thank you for the vids. Much appreciated friend.


I think the 3rd video is probably the best and like those above said, he uses syringe, just like other stuff be sterile in front of a flow hood etc...

:sun:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW] * 1
    #27932880 -

I had a PDA plate 95% colonized and then BOOM trych.//
Hate this shit especially since alot of my plates have grown over green mold spots (+these pink/orange bubble looking contams-I don't know whether they are bacteria or mold.) and now I'm faced with the decision to try and cut out the bad parts and save or just use them as skeet.


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Obvious spots of green mold there covered up with myc when held up to the light.(This is a different plate.)
----------

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Photos attached for evaluation and reference. Please tell me what to do O wise ones.
Thanks

PS that large strand in the middle of the plate in the second photo was a ribbon of myc i used to inoculate.


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

Edited by Then Transporeter (09/03/22 11:57 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter]
    #27932884 -

I've been trying to get my agar game going strong lately :mushroom2::thumbup:


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter]
    #27932915 -

Then Transporeter said:
I had a PDA plate 95% colonized and then BOOM trych.//
Hate this shit especially since alot of my plates have grown over green mold spots (+these pink/orange bubble looking contams-I don't know whether they are bacteria or mold.) and now I'm faced with the decision to try and cut out the bad parts and save or just use them as skeet.


----------
Obvious spots of green mold there covered up with myc when held up to the light.(This is a different plate.)
----------

----------
Photos attached for evaluation and reference. Please tell me what to do O wise ones.
Thanks

PS that large strand in the middle of the plate in the second photo was a ribbon of myc i used to inoculate.


Skeet Skeet Skeet it and video it. PULL!

When plates have otherwise normal(ish) agar but it's puffy, it often is not a good sign.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27932920 -

Thanks yeah i think I'll be getting rid of all of them. Just hate letting go of things I know I've put work into ya know.


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27932921 -

Quick question, you guys, hope you don't mind...

What do you guys do with leftover healthy, fully-colonized plates? For instance, a plate with a few wedges taken out for grains, but a variety you don't plan to use again for awhile

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Winston Smith] * 2
    #27932922 -

Stick em' in the fridge!


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter]
    #27932928 -

That easy? Nice! Thank you for the quick answer.

On average, assuming moderately ideal conditions are met, how long will they last in the fridge? Up to a year possible?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Winston Smith]
    #27932946 -

Winston Smith said:
That easy? Nice! Thank you for the quick answer.

On average, assuming moderately ideal conditions are met, how long will they last in the fridge? Up to a year possible?


In my reading here I've understood two things...

1. In the coldness of the fridge, it will somewhat stall the myc from conlonzing or feeding on the agar!?

2. I think agar and the myc share moisture, so when the agar dries out the myc might become weak to wistand bacteria or just without moisture the agar might dry up hence another reason the myc might become too old or die!?

I'm new and doing a bunch of reading and watching video's as am I on my first grow, but seems yea they could last a year especially if one used a slant with a wood popsicle stick for the myc to feed on!?

myc = mycelium

Masters of agar please inform me if I'm correct or not :stoned:...lol

:mushroom2:


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Mushroom Cultivation Techniques...

Edited by Grogu (09/04/22 01:06 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Winston Smith] * 1
    #27932950 -

Got a couple Melmak plates that started pinning. Do I wait for the plate pins to get bigger before transferring?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grind365]
    #27932992 -

I'm not an expert but i don't think it matters. Even the smallest pins still contain enough material to start growth on a new plate. In my experience though I've found letting them get a little bigger cuts back on colonization time slightly.


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter]
    #27933007 -

Then Transporeter said:
I'm not an expert but i don't think it matters. Even the smallest pins still contain enough material to start growth on a new plate. In my experience though I've found letting them get a little bigger cuts back on colonization time slightly.


So my thinking on the issue and the reason I ask is I believe the point of transferring a plate pin is to get a quick, clean clone culture.
While you can still do so with a small plate pin. I feel like it would be a lot easier with a larger one since you can easily differentiate between growth from the pin itself and the mycelium that will be transferred with the pin if the pin is larger.
Also if you have a larger pin you can take just the end of it instead of the lower portion that will still contain mycelium from the MS culture.
Or am I just completely overthinking here.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Winston Smith]
    #27933033 -

Winston Smith said:
That easy? Nice! Thank you for the quick answer.

On average, assuming moderately ideal conditions are met, how long will they last in the fridge? Up to a year possible?



I've revitalized after 7 months. Probably could have easily done more.

You can always take a transfer and then put them back in the fridge any time.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27933063 -

Hey there shroomies,

Quick question please, I'm trying to grow B+ on agar from a print. This looks pretty good to me but want to check this isn't some weird contam that I'm not aware of. Am I ok here?

Also, I'm going to do a couple of agar to agar transfers, am I close to the point at which I should do the transfers?


Thanks alot for all the advice!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter] * 3
    #27933086 -

Grind365 said:
Got a couple Melmak plates that started pinning. Do I wait for the plate pins to get bigger before transferring?



Then Transporeter said:
I'm not an expert but i don't think it matters.


Transporeter: It does matter.

You probably shouldn't give advice when you don't know, at least here.

It's OK to not comment. We are protective about this on here, calling that out is how we keep misinformation from spreading on here. It's part of why Shroomery is better than Reddit for grow advice. Any old b.s. is OK on Reddit.

If you have a culture in a plate that's not a clone, and you clone a plin (plate pin), you have a clone. That's how I got my strongest culture.

Your mileage may vary, but at least you'll get a grow that is  consistent in strength, and in when it fruits. Shrooms grown from spores aren't as consistent as clones. Clones are generally the goal here.

If you wait for a plin, and cut that without pulling any of the mycelium below it, and it hasn't dropped spores yet, cut it off with a flame-sterilized scalpel a tiny bit above the plate, that's the easiest way to clone.

And will likely be clean if your plate is clean.

Sometimes cloning a larger shroom grown in the open air will introduce contams. Though you can minimize the chance by cloning from inside the stipe or inside the cap.

Don't wait for plins to get too big, they'll drop spores. If that happens anywhere on the plate and you clone a pin, you're not sure it's a clone. What you grow might be starting over from spores, or have some of that.

Pick the best looking plins to transfer, either biggest (without dropping spores) or fattest, or fastest growing, or the first to appear. They don't have to "get bigger to cut down colonization time" though.

Welcome both of you.
and Grind365, where do you get those little Petri plates? how many cm across?  I want some.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (09/04/22 07:33 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27933170 -

Sorry day one stuff there. I'll keep it a hundred from now on. Best regards

PS I want some of those petri dishes too. I heard some people use the glass ones, but i've heard they might be more trouble than they're worth $ wise. What can I use other than a plastic petri dish other than a glad mini-round? (I do no-pour agar with those)


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter] * 1
    #27933172 -

Then Transporeter said:
Sorry day one stuff there. I'll keep it a hundred from now on. Best regards

PS I want some of those petri dishes too. I heard some people use the glass ones, but i've heard they might be more trouble than they're worth $ wise. What can I use other than a plastic petri dish other than a glad mini-round? (I do no-pour agar with those)



You can pour using little condiment cups.. I've gotten the ones from Walmart to pour in $3 for 50... or if you look up crack-plates I guess they can be reusable


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27933176 -

Like the little plastic restaurant ones? I've worked in restaurants 15 years and i've never thought of this lol.


--------------------
"I don't care what congress does, so long as they don't do it in the streets and startle the horses." -Victor Hugo

>>>LOVE YOURSELF........<3----------:mushroom2::heart::thumbup::thumbup::heart::mushroom2:--------;)........HELP OTHERS>>>

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Then Transporeter]
    #27933178 -

Then Transporeter said:
Like the little plastic restaurant ones? I've worked in restaurants 15 years and i've never thought of this lol.


Yeah.. as long as they're sealed, they should be sterile


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: Winston Smith]
    #27933225 -

Winston Smith said:
That easy? Nice! Thank you for the quick answer.

On average, assuming moderately ideal conditions are met, how long will they last in the fridge? Up to a year possible?


A few months depending on the thickness of the agar and they'll dry out like a thin cracker


--------------------

Links For Beginners And Beyond:mspoil:

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Shop: North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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