Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinegreymule
enthusiast
Registered: 03/25/01
Posts: 135
Loc: Colorado
Last seen: 11 years, 3 months
Re: why can't i get the agar thing right?
    #290788 - 04/12/01 01:53 AM (21 years, 5 months ago)
Log in to view attachment

My friend uses Malt Extract agar from fungi perfecti. It has a touch of antibiotic in it. He then supplements the malt extract agar with brewer's yeast, a touch of gypsum, spirulina and dog food. Lately he's also tried a little colloidal minerals that seems to spice it up nicely. He uses a small squirt from a syringe, and then rolls it around. Within 4-5 days @ 75F there's usually a dot started. He uses pre-sterilized petri dishes. He mixes the agar shit (it doesn't want to dissolve, but you can eventually wear down its resolve to not become part of the solution) with boiling water. Then boils it and stirs it to make sure it's dissolved. Then PC's it for 30 min. Then in front of a cheapo HEPA from W-Mart, fills the dishes. They're cool after about two hours (unfortunately he's still not cool). Then again in front of Mr. HEPA, with the petri dishes closest to the flow, he opens (tilts) the side of the dish closest to the HEPA and gives it a squirt. So far, the mycellium grow like crazy. I'm attaching a bit from Stamets w/o authorization.

Get behind the Mule every morning and plow!


--------------------
Them not busy bein' born are busy dyin'.


Extras: Filter Print Post Top
OfflineShroomzilla
enthusiast

Registered: 02/04/01
Posts: 177
Loc: Texas, home of the longho...
Last seen: 20 years, 6 months
Re: why can't i get the agar thing right?
    #291215 - 04/12/01 08:32 AM (21 years, 5 months ago)

I normally use the MPG [malt peptone grain] formula right out of TMC [see greymule's attachment] and it works like a charm. Only variation is to substitute rice flour for the rye if I'll be innoculating into rice based substrate.

If you start with spores you'll get fuzz 99% of the time. I almost always start with tissue from the base of the stem. Doing this at least 75% of the dishes are nice and rhizomorphic, rest are usually "intermediate", very few fuzz balls. Tissue from up in the cap seems to take off and grow faster, but fuzzes more.



Extras: Filter Print Post Top
Offlinemorlahere
addict
Registered: 08/16/00
Posts: 93
Last seen: 18 years, 8 months
Re: why can't i get the agar thing right? [Re: Shroomzilla]
    #291285 - 04/12/01 10:26 AM (21 years, 5 months ago)

thanks guys.

looks like i'm the only fool!
must be me. i'll just keep trying untill i get it right.
thanks again for the input.



Extras: Filter Print Post Top
Offlinegreymule
enthusiast
Registered: 03/25/01
Posts: 135
Loc: Colorado
Last seen: 11 years, 3 months
Re: why can't i get the agar thing right? [Re: Shroomzilla]
    #291379 - 04/12/01 04:14 PM (21 years, 5 months ago)

What is peptone and where do you get it?

Get behind the Mule every morning and plow!


--------------------
Them not busy bein' born are busy dyin'.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Potato Dextrose Agar X Malt Extract Agar Alegalize 5,285 8 02/25/05 08:03 PM
by agar
* AGar colonizatoin / Malt Extract liquid mycellium cookiewhore 2,292 2 01/28/02 03:57 AM
by cookiewhore
* Agar question sill_o_cin 3,640 4 11/22/01 02:23 AM
by sill_o_cin
* CASO and Sabouraud-Glucose Agar: Any good ? JeRiKo1 1,904 5 04/19/04 01:24 PM
by JeRiKo1
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 19,404 38 05/11/01 08:08 PM
by MAIA
* Agar's fun IGnosticAbhorI 2,246 14 06/27/05 06:21 PM
by IGnosticAbhorI
* Contest!!! Be an Agar Star
( 1 2 3 4 all )
egghead 8,199 68 05/28/01 12:33 AM
by m0ck5
* some questions about agar ar393 954 3 06/12/01 12:04 AM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, Tormato
766 topic views. 31 members, 130 guests and 40 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2022 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.011 seconds on 15 queries.