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OfflineAss_Pirate
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Registered: 03/28/05
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agar-agar
    #4147982 - 05/07/05 09:03 PM (12 years, 2 months ago)

for i think its MEA ...the one where you boil down potoato and add agar and stuff... why cant you just use PURE agar...?

or can u?
new at agar i just picked up some powderd stuff and really wanted to try it for edibles.  :thumbup:


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Offlineshroomguitar420
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Re: agar-agar [Re: Ass_Pirate]
    #4147997 - 05/07/05 09:06 PM (12 years, 2 months ago)

yeah, that you stole from you college lab lol


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Offlinebumper
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Re: agar-agar [Re: shroomguitar420]
    #4148020 - 05/07/05 09:12 PM (12 years, 2 months ago)

If you get straight agar it has no nutrients added, the agar used for growing mushroom cultures has added nutrition to it. Agar is just a jellying agent to hold the water and nutrients in.
As to the best of my knowledge.


--------------------
Pitbulls are like any other dog, they can be trained to be nasty or they can be trained to be good, some are smart and some are stupid, just like every other breed.
Their tough, stubborn and loyal, which is easily turned into something horrible in the wrong hands.


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InvisibleTien
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Re: agar-agar [Re: bumper]
    #4148033 - 05/07/05 09:17 PM (12 years, 2 months ago)

Quote:

bumper said:
If you get straight agar it has no nutrients added, the agar used for growing mushroom cultures has added nutrition to it. Agar is just a jellying agent to hold the water and nutrients in.
As to the best of my knowledge.




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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
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Re: agar-agar [Re: Ass_Pirate]
    #4148055 - 05/07/05 09:25 PM (12 years, 2 months ago)

Here's the scoop:

Agar is a polysaccharide derived from a marine alge, species Agar agar. It is used as a gelling agent because few microorganisms have the necessary enzymes necessary to break down the polysaccharide, which would turn it to a liquid mush and wouldn't be very useful for culturing isolates, observing characteristics or much else.

Basically, agar is non-nutritious and can have nutrients dispersed throughout the matrix, giving the microorganisms mechanical support to grow on.

This is why gelatin or pectin are rarely used for microbiology, because many microorganisms can digest these substances.

In some instances however, a microorganism may be able to digest agar, and a diferent media wil have to be used.

EDIT: Agar also has a different melting and solidifying temperatures which make it useful for high temperature incubation, where gelatin or pectin would be turned to liquid.


--------------------
Man thinks. God laughs. - Jewish Proverb


Edited by no-tone (05/07/05 09:26 PM)


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Offlinelordoftheshroomz
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Re: agar-agar [Re: no-tone]
    #4148096 - 05/07/05 09:43 PM (12 years, 2 months ago)

thanks professor :wink: :smile:


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Enema Bag Jones

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Re: agar-agar [Re: lordoftheshroomz]
    #4148116 - 05/07/05 09:50 PM (12 years, 2 months ago)

My pleasure!  :thumbup: :laugh:


--------------------
Man thinks. God laughs. - Jewish Proverb


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Offlinexburn
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Re: agar-agar [Re: no-tone]
    #4148841 - 05/08/05 01:44 AM (12 years, 2 months ago)

also MEA has n potatos that PDA.
MEA=
20g agar
20g malt
1l water


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Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.


Edited by xburn (05/08/05 01:45 AM)


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Invisibletahoe
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Re: agar-agar [Re: xburn]
    #4149205 - 05/08/05 04:20 AM (12 years, 2 months ago)

mea= malt extract agar
pda=potato dextrose agar
pda= public display of affection
The latter just being plain tacky


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflineAss_Pirate
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Registered: 03/28/05
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Last seen: 11 years, 9 months
Re: agar-agar [Re: tahoe]
    #4150922 - 05/08/05 08:28 PM (12 years, 2 months ago)

thanks SO much guys, i think i can handle it frm here i ll just read up on sterilizing and pouring instructions...and is it nessesary to have a glove box for this? cuz i sall the guy in those...vidoes on the forum(excuse me if u dont know what im talking about) and he just plops it in there

glove box?


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Offlineclumbsy_smurf
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Registered: 10/08/02
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Last seen: 12 years, 2 months
Re: agar-agar [Re: Ass_Pirate]
    #4150938 - 05/08/05 08:33 PM (12 years, 2 months ago)

glove box or flow hood. Without you greatly increase your chances of contams. You can build a workable one with a plastic tub and a clear trashbag, or even a cardboard box and a clear trashbag. It's not as good, but it'll get you by at the very start.


--------------------
i know i know, i dont know how to speel, get off my back alrady.


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Enema Bag Jones

Registered: 10/17/00
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Re: agar-agar [Re: Ass_Pirate]
    #4151075 - 05/08/05 09:35 PM (12 years, 2 months ago)

I haven't seen the videos, but i would assume he did it outside andy glove box or flow hood so it would be visible for the camera.


--------------------
Man thinks. God laughs. - Jewish Proverb


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