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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Danny McBride]
    #27911316 -

Danny McBride said:
So basically I made a transfer from this plate some time ago because it had signs of contam not sure why but I have been keeping all of my plates even the contaminated ones which is probably a bad practice but anyways I got my first ever mushroom from this contaminated plate. I am so excited and hyped like AGGGGHHHHHH First ever shrooomie even though its super small its super exciting for me!

I suppose I am going to clone it, Should I drop the whole pin onto a new agar dish or cut it up and drop onto a few dishes or I I slice it in half and take a small sample from the middle?



i wouldnt touch this plate with a 10 foot pole


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helpful links
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27911319 -

Rotnpins said:
For those of you who know about the Tidal Wave culture that I've been having terrible luck with (it was enmeshed with something that would even ride along with plate pins)



I think I finally got it clean :dancer: but I'd like some second opinions, incase there is some indication that it's still enmeshed with something I should be looking for that I'm missing.

*the spark spot in the center is from charcoal donor plate



looks good to me :super:


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helpful links
my FH build

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27911374 -

Is that the culture you cleaned on water agar? Looks good:thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27911380 -

Rotnpins said:
For those of you who know about the Tidal Wave culture that I've been having terrible luck with (it was enmeshed with something that would even ride along with plate pins)



I think I finally got it clean :dancer: but I'd like some second opinions, incase there is some indication that it's still enmeshed with something I should be looking for that I'm missing.

*the spark spot in the center is from charcoal donor plate


I am too green to be offering advice but both times I have gotten swabs of TW I have had the same issue. Myc looks like a puff ball and unevenly and slowly grows out and even pins on the plate. Something was even on the plins as I could never get it clean. Hopefully somebody has some cleaner spores out there. I would like to have some success with it and hope you do as well.


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R.I.P. Dad - I will love you and keep you in my heart and memories always.
01/26/46 - 04/10/22

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27911452 -

Rotnpins said:
For those of you who know about the Tidal Wave culture that I've been having terrible luck with (it was enmeshed with something that would even ride along with plate pins)



I think I finally got it clean :dancer: but I'd like some second opinions, incase there is some indication that it's still enmeshed with something I should be looking for that I'm missing.

*the spark spot in the center is from charcoal donor plate


Me me me over here ya tidalwave have been hard for me lots of lost plates,pf jars,oat jars, and spawned sub to SB.  Once the SB I have flushes out I'm down with tidal wave.

@Danny McBride that isn't a plate pin that's a full grown shrooms.  It's dropped it's spores so like monkey_accident said toss it


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27911472 -

Rotnpins said:
For those of you who know about the Tidal Wave culture that I've been having terrible luck with (it was enmeshed with something that would even ride along with plate pins)



I think I finally got it clean :dancer: but I'd like some second opinions, incase there is some indication that it's still enmeshed with something I should be looking for that I'm missing.

*the spark spot in the center is from charcoal donor plate


Oh shit! Finally! Lol
:hahyeahwoo:


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc] * 1
    #27911755 -

Amazing what happens when you don't touch things for over a month



becomes (those slices turned into a wonderful pair of shoeboxes that are on their second flushes now!)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Danny McBride]
    #27911863 -

Not the most ideal candidate to clone but the best practice would be to rip the mushroom in half lengthways then cut a section from the middle of the stipe (trunk) to transfer to agar.
To open the mushroom to get to the clean contamination free part of the stipe, ripping is better than cutting as prevents pushing any contamination outside of the mushroom into the clean internal you will take the clone from.
Could prove tricky with the little guy you have but nothing ventured nothing gained.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Markamello]
    #27911956 -

Markamello said:
Not the most ideal candidate to clone but the best practice would be to rip the mushroom in half lengthways then cut a section from the middle of the stipe (trunk) to transfer to agar.
To open the mushroom to get to the clean contamination free part of the stipe, ripping is better than cutting as prevents pushing any contamination outside of the mushroom into the clean internal you will take the clone from.
Could prove tricky with the little guy you have but nothing ventured nothing gained.


I'm not sure who you are talking to. However if you are referring to that plate pin that sounds like a lot of work when you can just transfer the pin to another plate. Of course if you want it to be a true clone you will have to take just the pin and none of the mycelium it is attached to but no need to clone from inner tissue on a plate pin.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grind365] * 1
    #27911973 -

Grind365 said:
Markamello said:
Not the most ideal candidate to clone but the best practice would be to rip the mushroom in half lengthways then cut a section from the middle of the stipe (trunk) to transfer to agar.
To open the mushroom to get to the clean contamination free part of the stipe, ripping is better than cutting as prevents pushing any contamination outside of the mushroom into the clean internal you will take the clone from.
Could prove tricky with the little guy you have but nothing ventured nothing gained.


I'm not sure who you are talking to. However if you are referring to that plate pin that sounds like a lot of work when you can just transfer the pin to another plate. Of course if you want it to be a true clone you will have to take just the pin and none of the mycelium it is attached to but no need to clone from inner tissue on a plate pin.



It looks like there is sporelated mold on the plate.. I'd personally cut the plin open to increase chances of a clean sample.

It's not as tricky as markamello suggests though.. just use some sterile Tweezers to rip open the plin.


--------------------
Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 3
    #27911978 -

FP+ tissue culture T1 about to go to grain in another day or two.


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There are only 10 types of people in the world, those that understand binary and those that do not.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen] * 1
    #27912016 -

Lookin nice :headbanger:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grind365]
    #27912032 -

Grind365 said:
Markamello said:
Not the most ideal candidate to clone but the best practice would be to rip the mushroom in half lengthways then cut a section from the middle of the stipe (trunk) to transfer to agar.
To open the mushroom to get to the clean contamination free part of the stipe, ripping is better than cutting as prevents pushing any contamination outside of the mushroom into the clean internal you will take the clone from.
Could prove tricky with the little guy you have but nothing ventured nothing gained.


I'm not sure who you are talking to. However if you are referring to that plate pin that sounds like a lot of work when you can just transfer the pin to another plate. Of course if you want it to be a true clone you will have to take just the pin and none of the mycelium it is attached to but no need to clone from inner tissue on a plate pin.


Yeah I'm replying to the post about the pin on the plate covered in mold. If the pin was from a clean plate no worries transferring it straight to agar but if you took that pin and put it to agar I'd put my money on transferring contam with it.

I think it would be a little tricky only because the pin is so small

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Markamello] * 3
    #27912249 -



Hi, my name's Psycho and I'm an agarholic.


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Markamello]
    #27912333 -

Markamello said:
Grind365 said:
Markamello said:
Not the most ideal candidate to clone but the best practice would be to rip the mushroom in half lengthways then cut a section from the middle of the stipe (trunk) to transfer to agar.
To open the mushroom to get to the clean contamination free part of the stipe, ripping is better than cutting as prevents pushing any contamination outside of the mushroom into the clean internal you will take the clone from.
Could prove tricky with the little guy you have but nothing ventured nothing gained.


I'm not sure who you are talking to. However if you are referring to that plate pin that sounds like a lot of work when you can just transfer the pin to another plate. Of course if you want it to be a true clone you will have to take just the pin and none of the mycelium it is attached to but no need to clone from inner tissue on a plate pin.


Yeah I'm replying to the post about the pin on the plate covered in mold. If the pin was from a clean plate no worries transferring it straight to agar but if you took that pin and put it to agar I'd put my money on transferring contam with it.

I think it would be a little tricky only because the pin is so small


Cool yea. I'd take my luck and just transfer away from any contams that come along for a ride. Knowing my luck I'd fk it all up if I tried to take a inner tissue sample from a pin. Doesn't sound fun. I've messed up plenty of times just trying to clone using inner tissue on a mature mushrooms.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen] * 1
    #27912340 -

JXAllen said:
FP+ tissue culture T1 about to go to grain in another day or two.



:awesomenod: hell yeh


--------------------
helpful links
my FH build

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JXAllen] * 2
    #27912391 -

JXAllen said:
FP+ tissue culture T1 about to go to grain in another day or two.



That is a hella clean T1.  Nice work.


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“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021

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Re: AGAR ENVY! (Anything and All things agar!) [Re: jay.ach]
    #27912470 -



I have had successful transfers from plates that have contams on them.  Dont cross over the contam when sampling/transferring from your shitty plate, and work as cleanly as possible.  Heck, Id even opt in for a Josex poke to PF on a nasty plate with some halfway good growths on them if I wanted to experiment or save them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 3
    #27912716 -

SirPsycho said:


Hi, my name's Psycho and I'm an agarholic.


I think they wrote a book you can read for that! AA agarholics anomynous maybe useful for a grow.:cheers:


--------------------
BEWARE OF PICK POCKETS AND LOOSE WOMEN!!
"Life shouldn't be a journey to the grave with the intentions of arriving safely in a well preserved body, but rather skid in broadside in a cloud of smoke throughly used up totally worn out and loudly proclaiming WOW WHAT A RIDE!"
Hunter S. Thompson
"I want to die peacefully like my grandfather in his sleep, not yelling and screaming like his passengers." Bob Monkhouse
" Sticking feathers up your ass does not make you a chicken!" Tyler Durden
"He who makes a beast of himself gets rid the pain of being a man." Samuel Johnson
The noob forum
Crack Plates
Fastfreds media cookbook 60 agar/lc recipes
Water tub fruiting chamber Top layer/Pseudo casing Complete agar resource guide
Trusted teks and linksSEED "PETRI" TEK Vent ur PC

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Re: AGAR ENVY! (Anything and All things agar!) [Re: wild_aussie]
    #27912929 -

Does anyone use grain water with other nutrient sources?

I'm looking to try another type agar besides my usual LMEA.

I was reading through some teks and whatnot and saw that one TC recommended using grain soak water diluted while another recommended using it full strength. Then it was mentioned that it was beleived different grains have different strengths of soak water as in oat soak water is much stronger than millet soak water.

I want to use my grain water diluted to increase clearness in plates. My grain water is super dark and cloudy from gypsum. Yes I still use gypsum I have over 100lbs of the stuff so I use it when I remember to add it.

I am thinking about doing 250ml grain soak water 250ml tap water 4gs lme and 8gs agar. Does that sound like it would possibly work or should I try something else. I have currently have access to karo, lme, potatoes, potato flakes, and dog food. I did read that Pasty liked to use grain soak dog food agar so I'm not opposed to trying that either.


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Edited by Grind365 (08/21/22 10:20 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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