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Glass Petri Agar Tek * 7
    #27292936 -

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Step 1:



Pull around 16 inches of regular foil and put it on your table. Take a stack of 8 clean glass petris and lay it on one end of the foil. Begin rolling the stack up and tucking the ends of the foil as you go to keep the stack tight. Wrap the stack up all the way and neatly press the foil down so it can stand up like a cylinder.


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Step 2:



Place the stacks directly on your upper oven rack and turn it to bake at 350F. Let the plates "cook" for an hour and turn the oven off. Allow the plates to cool in the oven for a few hours before you get ready to mix your agar recipe


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Step 3:

 

Now we can mix our agar recipe. Figure out how much agar you need, for these 90mm petris I will use about 25ml per plate and will be doing 24 plates, so I need 600ml of agar. Standard MEA is my preferred recipe which at this ratio would be 600ml water/12g Agar/12g Light Malt Extract. Add the water to your pan and then dump in the dry ingredients. Turn the heat to high and stir constantly while you bring to a simmer and carefully remove from the heat while you grab your stick blender. Put it back on the burner and hit it with the stick blender for 30 seconds to really dissolve everything.


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Step 4:



Get your glass media bottles ready.  In my case I am using motherfuckin Boone's Farm bottles. Someone's getting laid tonight. Split your agar among the bottles so that they don't boil over. At this time add any coloring or antibiotic additives. I added a tsp of activated charcoal to each here. Cover the media bottles with foil tightly. No lid, just foil.


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Step 5:



Put your media bottles in your pressure cooker and PC for 30 minutes at 15psi. Move PC to your flowhood to cool


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Step 6:



Allow the cooker to cool off for 1 hour. Then remove your media bottles and place them on a rack in your flowhood air stream. Go and fetch your foil stacks of plates and put the stacks in a ziplock to transport to your flowhood area if necessary.


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Step 7:

Depending on how much media is in the bottles and your ambient temps, allow the agar bottles to cool off in the air stream a little longer so that they are still warm but not too hot. Maybe 5-10 minutes and they should be good.


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Step 8:



When the temp has dropped enough and you are ready to pour, grab a foil pack of plates and carefully unwrap it and place the stack on your rack. Only work with 1 stack at a time. 8 plates, 2 stacks of 4. You can lift 4 plates with 1 hand easily to pour. So place them 4 and 4 next to each other on your rack with enough room between the two stacks the your hand will not interfere with the stack next to it and air wont blow off of you into it.


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Step 9:



Remove the foil lid on your media bottle, carefully and cleanly ripping it off. Starting at the bottom of one of the stacks of 4, grab the lid and lift the stack up leaving the bottom. With your other hand swirl the agar media bottle and then carefully pour the warm agar into the bottom dish. Lower the stack back down on top of this poured plate. Now move up to the next dish and lift the stack off leaving only it's bottom and repeat the previous pour. Repeat on all of your dishes until you are done with those 8.


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Step 10:





Open your next pack of 8 and place 4 and 4 right on top of the previous poured plates. Repeat the process of step 9 and 10 until you have completed pouring.


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Step 11:

Allow the stacks to cool in the flowhood for several hours. At this point you have successfully poured glass plates sandman style. You can now move the plates to large ziplocks for storage or use them right away.


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--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27292958 -

Could I get away with doing this tek without a flowhood and using a SAB in its place?
Im about to make my first round of agar and I really like the look of this but can I replace the word "flowhood" with "put inside your SAB" and still get generally good results?


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.
ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

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Re: Glass Petri Agar Tek [Re: Ashtray161]
    #27292969 -

I don't recommend pouring plates in a SAB. You can't really have still air with hot agar in there. Expect a heavy contm rate if you do that. You would be better of doing no-pour style agar teks.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27292976 -

sandman420 said:
I don't recommend pouring plates in a SAB. You can't really have still air with hot agar in there. Expect a heavy contm rate if you do that. You would be better of doing no-pour style agar teks.


FoSo, that makes sense. Hot air=turbulent air, right? awesome write up btw


--------------------
(You Know What Time It Is)
First they came for the communists, and I did not speak out because I was not a communist.
Then they came for the migrants, and I did not speak out because I was not a migrant.
Then they came for the queers, and I did not speak out because I was not queer.
Then they came for me, and there was nobody left to help me.
ABOLISH ICE. Free Palestine. Revolution or bust (A) . Profile pic credit goes to an awesome human
Be Gay, Do Crime 161 1312

Edited by Ashtray161 (05/03/21 08:49 PM)

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Re: Glass Petri Agar Tek [Re: Ashtray161]
    #27292978 -

yessir you got it. Thanks.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Re: Glass Petri Agar Tek [Re: sandman420]
    #27293012 -

sandman420 said:
I don't recommend pouring plates in a SAB. You can't really have still air with hot agar in there. Expect a heavy contm rate if you do that. You would be better of doing no-pour style agar teks.


I'm not the most experienced dude in the world but I've poured 100 plates in a SAB over the last three or so months and have lost only 2 plates to contam... seems to work fine :shrug:

In any case nice tek, I'm definitely interested in switching to glass. Kinda cool you can sterilize them in the oven, too.


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Re: Glass Petri Agar Tek [Re: Benson]
    #27293081 -

There are certainly some people that seem to get away with it ok, I reckon it depends on your ambient spore loads and how dirty your house is. A lot of stuff doesn't necessarily work for everyone. I had about 20% random contams on average pouring in a SAB personally.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27293105 -

How did you make the weird knot thing at the top of the post?

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Re: Glass Petri Agar Tek [Re: aah] * 1
    #27293113 -

it's ascii art that I copy and pasted haha. I could only get it to work if I did it in the "code" text button though so i couldn't color it :frown:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Re: Glass Petri Agar Tek [Re: sandman420]
    #27293183 -

sandman420 said:
There are certainly some people that seem to get away with it ok, I reckon it depends on your ambient spore loads and how dirty your house is. A lot of stuff doesn't necessarily work for everyone. I had about 20% random contams on average pouring in a SAB personally.


Yeah that's a good point


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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27293377 -

sandman420 said:
I don't recommend pouring plates in a SAB. You can't really have still air with hot agar in there. Expect a heavy contm rate if you do that. You would be better of doing no-pour style agar teks.


Ive poured hundreds and hundreds of dishes in a SAB. In a carpeted living room with animals around


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Glass Petri Agar Tek [Re: bodhisatta]
    #27293394 -

Nice write up.

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Re: Glass Petri Agar Tek [Re: DERRAYLD] * 1
    #27307239 -

Thanks D

If anyone is wondering why I don't sterilize the plates with the agar, it's because the plates come out dry as a bone from the oven.

:teabird:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27307298 -

Is popping agar dishes in the oven the only way to sterilize them before pouring?

I actually just got glass dishes recently and was curious


--------------------
100% Professional Psychonaut
Trippin' Aint Easy



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Re: Glass Petri Agar Tek [Re: Skizor1337]
    #27307307 -

I used to do it in the PC but like I said above they come out damp. It works fine just makes it harder to see. Also sometimes the damp plates stick together with surface tension and I have broken a whole stack once trying to get it free :cheers:

You could buy a toaster oven for cheap at goodwill if you don't have one.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (05/13/21 05:16 PM)

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27307312 -

I pour glass petri plates in my SAB.
Sometimes it's good, sometimes not.
Last time made me sad, 3 clean dishes out of 20. I guess I was getting cocky.
Don't be in a rush.


--------------------
“The strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”

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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27307315 -

sandman420 said:
Thanks D

If anyone is wondering why I don't sterilize the plates with the agar, it's because the plates come out dry as a bone from the oven.

:teabird:


I needed to hear about this.


--------------------
“The strength of a person's spirit would then be measured by how much 'truth' he could tolerate, or more precisely, to what extent he needs to have it diluted, disguised, sweetened, muted, falsified.”

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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27391420 -

So instead of using a oven (that we need to have for many other things) to do agar like a normal person you gotta buy some extra shit to maybe (but definitely wont) get the same results?

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391423 -

Just adding another "I've poured 100s of plates in a SAB with no issues" into the mix.

Nice write up! Anything with ascii art gets my vote!

Silkie_chicken said:
So instead of using a oven (that we need to have for many other things) to do agar like a normal person you gotta buy some extra shit to maybe (but definitely wont) get the same results?


Your post confuses me. What are you trying to say?


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391424 -

Sane person may be better said.

I'm not trying to bring you down or anything just bringing conversation, how is it worth it to go buy a dork ass toaster machine to do agar when we all have ovens

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391426 -

Silkie_chicken said:
Sane person may be better said.

I'm not trying to bring you down or anything just bringing conversation, how is it worth it to go buy a dork ass toaster machine to do agar when we all have ovens


Haha, are you suggesting pouring plates in the oven rather than using a flowhood or a SAB??


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Glass Petri Agar Tek [Re: coversall]
    #27391435 -

coversall said:
Just adding another "I've poured 100s of plates in a SAB with no issues" into the mix.

You can't argue with someone who knows everything

But I like your style, good on you

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Re: Glass Petri Agar Tek [Re: Silkie_chicken] * 1
    #27391459 -

He's butthurt because I said his UV agar sterilization experiment was pointless. Haha. Nice, my very own hater. He really waited and tried to shoehorn my own words for a while. Pretty funny and dumb all at the same time.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391460 -

No argument here.

:asianofapproval:


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..

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Re: Glass Petri Agar Tek *DELETED* [Re: coversall] * 1
    #27391465 -

Post deleted by bodhisatta

Reason for deletion: Cleaning this up

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391470 -

If a mod could clean this thread up from the off-topic discussion I would appreciate it

Do you not own an oven in your home? That is very sad. I hope you ca get proper accommodation soon. Poverty is no joke.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek *DELETED* [Re: sandman420]
    #27391520 -

Post deleted by bodhisatta

Reason for deletion: Cleaning this up

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Re: Glass Petri Agar Tek *DELETED* [Re: Silkie_chicken]
    #27391532 -

Post deleted by bodhisatta

Reason for deletion: Cleaning this up


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Re: Glass Petri Agar Tek *DELETED* [Re: sandman420]
    #27391569 -

Post deleted by bodhisatta

Reason for deletion: Cleaning this up

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391581 -

I'm reminded of the Golden teacher Rule...
Do unto others' shrooms as you would have them do unto someone else's shrooms' mom.


--------------------
It's so easy to laugh
it's so easy to hate
It takes guts to be
gentle and kind
-the smiths

Your ego is your nemesis.
Seek the truth tirelessly,
Even at the cost of your own sanity.
-fiddle_head

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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391582 -

Yes it certainly is sad that you think this is the same thing as me saying you are not going to revolutionize agar with UV lights.

You see... I had a point, you are just being uncool because you are mad.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Re: Glass Petri Agar Tek *DELETED* [Re: sandman420]
    #27391585 -

Post deleted by bodhisatta

Reason for deletion: Cleaning this up

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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27391586 -

Nice write-up. I have a stack of glass petris I never use because it's always seemed like extra work, but I like this.


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Re: Glass Petri Agar Tek [Re: Silkie_chicken]
    #27391592 -

Dont tell me what to do, this is a cultivation forum and I love to discuss it. If you can't compete on the marketplace of ideas then fuck off. If you will look back on your thread I offered plenty of explanation and certainly wasn't bullying you. In fact I was joking around with you and your meme and then you got defensive and mad and I had to whip the shit out of you. I help a lot of people and I don't mind spanking out of line punks so occasionally we will run into a problem like this. I know many of you enjoy my technical experience so I don't get upset when a hater pops up. I'm not here to impress anyone just talk shit about growing shrooms.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27391669 -

Nice write up! Takes out a web order and a trash run!

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Re: Glass Petri Agar Tek [Re: Silkie_chicken] * 1
    #27392202 -

Silkie_chicken said:
Sane person may be better said.

I'm not trying to bring you down or anything just bringing conversation, how is it worth it to go buy a dork ass toaster machine to do agar when we all have ovens


Then use your oven.

Toaster ovens are way smaller and energy efficient. Preheat a whole oven for some dishes? Sure if it's all you've got. I use my toaster oven more than my oven soI don't heat up my whole house for some taquitos.

Why come to a thread just to be a dick?


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Glass Petri Agar Tek [Re: bodhisatta]
    #27602251 -

I’m thinking about switching to glass to cut down on plastic waste.

Once you sterilize in the oven wrapped in foil, can you store them longer term in a ziploc until you’re ready to pour?

How long do you think is too long to let them sit before pouring?

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Re: Glass Petri Agar Tek [Re: Scrimshaw]
    #27602284 -

Hi Sandman, sorry about the hater. Kinda like getting the crabs.

I let my plates sit in the SAB a few days, still wrapped in foil
and got a higher contam rate. That was one time so not a lot of
data, but I don't have a problem sterilizing the plates, sterilizing the agar,
and pouring in one day. It fits nicely together as the day progresses.

The best part about glass plates is feeling like there's no resistance to
burning through plates, they just come and go.


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: Glass Petri Agar Tek [Re: Inthepit]
    #27602298 -

I bought a small tote just to store sterilized "foil sleeves" until use and another with gaskets to store poured plates, but a ziplock could work just fine too. Make sure it's cooled off enough before putting it in there! :cool:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27602304 -

sandman420 said:
Make sure it's cooled off enough before putting it in there! :cool:


Haha of course. Thanks for the tek and the storage advice. I’ll probably give this a try in the next couple of weeks.

I ordered 10 cheap glass Petris but the quality leaves something to be desired. The gaps are big and the lid sizes are all over the map. Some sit flush to the bottom. Others are closer to the plastic plates. I was trying to avoid spending the $$ on Pyrex/Corning brand, but I’ll soon see if these issues are a big enough deal to live with or not.

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Re: Glass Petri Agar Tek [Re: Scrimshaw]
    #27602314 -

I prefer the cheap shit ones honestly. They are a little thicker and the closer tolerances on the Pyrex causes an occasional vacuum lock.

The gap on glass plates are a little tricky to wrap at first but you get the hang of it. Instead of wrapping the side middle you wrap the middle of the pararfilm into the bottom corner at a 45 degree angle. I need to add that detail to the tek but it's hard to photo that without help.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Agar Tek [Re: coversall]
    #27619298 -

coversall said:
Nice write up! Anything with ascii art gets my vote!




Ahhhh reminds me of my old Tandy 1000 and my 486...Yes I am old enough to remember coding in the ancient MS-DOS days with QuickBasic, Turbo Pascal, C/C++ IDE, Assembly Language, and of course debug.com. ASCII art was your only GUI restricted to 1, 4, or if you were lucky 16 colors. Thought I would give props to those who remember computers&programming before drivers and plug-n-pray when you had to do all the low and high level stuff yourself. Sorry to be off topic but it brought back memories when programming was truly fun...and now back to your regularly scheduled thread. :grin:

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Re: Glass Petri Agar Tek [Re: sandman420]
    #27619322 -

I'm still on that no pour glass petri, I do them in large pp containers with massive polyfil stuffed lid, 10 petris at a time and leave them to cool inside the container.
When I'm ready to work I open the large pp container in front of the flow.

I've been prepping dishes and leaving them for 3 weeks in the pp container and all traces of condensation dries off.

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Re: Glass Petri Agar Tek [Re: DERRAYLD]
    #27619348 -

Great tek sandman, I followed this to the letter for my first time pouring agar in petri’s (been doing no pour since I started). I think they turned out okay! I think I poured them a little thin but I guess you get used to how much to pour. Kinda hard in an SAB to see. This was the end result: PDYA (first time with this) and a pinch of activated charcoal


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Natedawgnow’s Mush Cult Hacks Post


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Re: Glass Petri Agar Tek [Re: sandman420] * 1
    #27674598 -

boone's farm! :rockon:

:drunkdriver:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28044744 -

Just trying to get clear on how to store poured plates that aren't going to be used for a while.

I've been wrapping poured plates which is a nuisance as I have to unwrap then wrap again after inoculation especially as one of the reasons I got into glass plates was to avoid plastic waste.

What other options are there for storing poured plates pre-inoculation?

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Re: Glass Petri Agar Tek [Re: bongoman]
    #28044745 -

In zip lock bags but I still think wrapping them is safer either way.
Or you choose pp containers that have a good lid seal.

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Re: Glass Petri Agar Tek [Re: DERRAYLD] * 1
    #28044995 -

I just wrap em until i need to use them. Then i just grab what i need. Tried a bunch of other methods over the years and have settled one just wrapping them.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28046049 -

Do you use the same piece of plastic to wrap it back up? Not sure if it would have a higher rate of contam at that rate or if you can just wipe it down with alcohol prior to starting.

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Re: Glass Petri Agar Tek [Re: ljcraig0804]
    #28046393 -

Fresh piece of parafilm everytime


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: Anonymous]
    #28048038 -

I did a batch of no-pour glass petris the other day and wrapped them after letting them cool and dry in front of the flow hood.

I then stored them in a tote box but on checking them today I noticed that many of the wraps have slipped off, underneath the plate.

Has anyone else experienced this? Note that the tote box hadn't been moved or bumped over the last couple of days. The plates were seemingly dry when I wrapped them.

I used grafting tape to wrap. Maybe I stretched it too tight? Maybe I wrapped it too low on the plate to cover the gap?


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Re: Glass Petri Agar Tek [Re: bongoman]
    #28048041 -

i just use seran wrap for mine & it sticks super well. if they are wet it’ll slip off but idk i’ve never used grafting tape. seran wrap works great tho


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28048091 -

Yeah, I'll give saran a try. It's really weird how the wrap just slipped off without any handling of the plates. Maybe temperature shifts and some moisture and me wrapping them too tight and low?

I've rewrapped them and will leave for a few days to see it they contaminate.

Edited by bongoman (11/13/22 01:21 PM)

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Re: Glass Petri Agar Tek [Re: bongoman]
    #28048094 -

yeah sometimes the wrap placement makes a difference with those factors but it is weird they slipped off. not familiar with grafting tape really but you shouldn’t have a problem w seran wrap


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28048097 -

Yeah the point is, the dishes must be dry. Otherwise the clingewrap won't hold propperly.

You can buypass that prob with just using Parafilm :laugh: It's just much more expensive compared to clinge. I like to use clingewrap when wrapping up dishes that I store in the fridge for later usage. The dishes I cultivate in are wrapped in Parafilm (as long as I have, if it's out I also use clinge)


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
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Re: Glass Petri Agar Tek [Re: Shrimps] * 1
    #28048374 -

Cling wrap is for the kitchen. Parafilm is for the lab. I've never had that problem with parafilm.

#ParafilmGang


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid] * 1
    #28048560 -

NOT NOW LOTKID !!


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse] * 2
    #28048571 -

Just sayin... :hehehe:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28056856 -

I've left a batch of foil-wrapped plates in the oven to cool down.

How long can they be safely left there? They are double-wrapped in the foil and had two hours at 430F.

My oven isn't that clean so am concerned about whether leaving them there until I get home is a vector for contamination.

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Re: Glass Petri Agar Tek [Re: bongoman]
    #28057108 -

You're good man

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28058794 -

LotKid said:
Cling wrap is for the kitchen. Parafilm is for the lab. I've never had that problem with parafilm.

#ParafilmGang


I am just starting out and have both parafilm that I haven't used yet and Saran wrap which I have.  I do my work in a virtually unused bathroom downstairs.  It's still, quiet and I can keep it really clean with little effort.  But it ain't a lab.  Decisions decisions :laugh:

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Re: Glass Petri Agar Tek [Re: sandman420]
    #28058909 -

Try both, see which you like best. My money on cling wrap


--------------------
Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: Glass Petri Agar Tek [Re: Rusty2096] * 4
    #28059054 -

Cling wrap is for keeping leftover uncle ben's fresher longer... :laugh2:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid] * 1
    #28059070 -

Ironically reading about the infamous Uncle Bens "tek" brought me here.  Thankfully I ate the agar pill and went that direction. Thanks to you all.

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Re: Glass Petri Agar Tek [Re: MycOnABike] * 3
    #28059717 -



:lmafo:


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28076703 -

Thanks for the tek, I've had endless contaminations by way of pressure cooking to sterilise dishes.

I assume a lot of my contamination vectors came from the dishes being wet on the outside (possibly facilitating bacterial contamination), vacuumed and very cumbersome to deal with. I look forward to trying out using an oven for sterilisation instead.

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Re: Glass Petri Agar Tek [Re: Effigy]
    #28164872 -

I just found this post after trying a no-pour with glass dishes in stainless sleaves. It was a total shit show. Everything is so wet! SO WET!

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Re: Glass Petri Agar Tek [Re: sandman420]
    #28576454 -

Thanks man, been looking for this thread for the past 3 weeks! Read it all, I feel enlightened :tongue2:

Sorry you had to put up with this hater, but hey it's kinda like having your own stalker... in a way it's a compliment!

So I'm a beginner and spent the past 3 weeks trying all kinds of teks. I use a sab and glass dishes. Best result I got contam wise was no-pour, but too messy and condensation was wild.

I then came across scientific papers and forums that swear you can use your microwave, both for dishes and agar. So I did. Over and over. Dropped MWing agar, for which I went back to PC, but kept using it for dishes cause they come out dry and clean.. and every time I think it's all good, I end up disappointed.

So does everybody on shroomery agree that microwaves are BS in all possible ways? Sorry I'm a noob, and the convenience and cleanliness were attractive...

The weird thing is that the best results I thought I got looked promising. Super clan and condensation free plates, poured in my sab, pristine after 4 days. But as soon as I inoculate them, next day I get this bacteria spreading UNDER the agar!  It's been happening pretty systematically. You'd think if under, it should have been there from the start (pre inoc), and I don't understand how inoculating on top of the agar can trigger bacteria underneath.. I have this little gif that shows parallax between the bacteria and myc. I also checked physically and it's definitely underneath...

I'll drop microwaving anything right now that I found this thread, but just curious if anyone has an idea what happened there?

Going oven right now.. the only thing is it does seem like a waste heating up my big oven for the small batches I make (5 to 10).. maybe will look into buying that roasting oven as mentioned. And hopefully won't trigger another wave of haters!!! :laugh2:


--------------------


Reaching for the summit, one way or another :leaving:



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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28576526 -

no pour glass petris usually dry out pretty well if you have the right containers or leave them on the shelf in foil long enough. you can let them dry out in an SAB for a day or two as well. might be worth trying no pours again if you can’t get pouring them to be consistent

sounds like your dishes are somehow getting bacteria in them at some point between sterilization & pouring. maybe your hands hover or pass over the dish in a way that allows bacteria to fall in while you’re handling them or pouring. i don’t know anything about microwaving agar but as far as im aware it is not at all common practice for us here & i wouldn’t recommend you do it when the PC is so easy & a guaranteed way to make something sterile. it could be some part of your technique or sterilization process but there’s no reason to add the microwave into the mix of possible contamination vectors


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28576642 -

I see where I went wrong with no-pour, thank you! I let my stack cool down and then stuck it in the fridge, which I guess trapped the condensation in… I also used wide painter’s tape, but you mention foil. Is that the same foil method as the oven one? As in rolling your stack in foil?

When you say to let them dry out in my sab, is that the whole stack still in foil or individually?

Yes I see what you’re saying about bringing in contam during handling or pouring. What puzzles me is that my plates stay clean for several days while I let them dry in my sab.. The bacteria under the agar only starts showing right after inoculation (less than a day), if it was there all along shouldn’t it have started spreading during the previous drying days?

Also during those drying/testing days I let them be as is stacked, no sealing. Should I seal them as soon as they’ve dried up instead?


--------------------


Reaching for the summit, one way or another :leaving:



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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28577024 -

oh yeah man the fridge is a nightmare for condensation lol there's no reason to keep blank plates in the fridge ever imo. they sit just fine on the shelf. no idea where you were using painters tape but yeah i wrap my no pours in foil just like you would for the oven, except there's agar in them & they go in the PC. the glass petri link in my sig is how i do them from start to finish if you need more details

i would unwrap them in stacks if you were gonna dry them in the SAB. they should be fine to sit in the foil on the shelf for awhile. i've had them dry out pretty well just chillin on the shelf but it might take a few days or more before they really get dry that way. last time i did a foil wrap, the foil was juuuust short enough to leave the top plate exposed on the top so you could see down into it as if you were using it & i think that helped quite a bit with drying out. the foil wrap generally just has to be sturdy enough to keep them from falling over, doesn't have to be perfectly sealed or anything

yeah i see what you're saying, that is weird. it's possible the timing is just super coincidental but it does seem correlated to you inoculating them. maybe do some blank transfers to see if the problem still pops up, & also leave some plates completely blank as a control? if it's not in there before you transfer it must be getting brought in when you do. is the bacteria right under where you drag your scalpel? or does it end up all over/on random parts of the plate?

if they stay in your SAB they should be fine to stay unwrapped, but if they are coming out of your SAB at any point i'd wrap them. i've done some limited testing with unwrapped glass & they don't seem to NEED to be wrapped, but handling them properly can be really tricky without a strip of cling wrap/parafilm around the bottom edge. if you have stacks of petris you won't use for awhile you can wrap them in a full sheet of seran wrap & cut off the petris you wanna use. but leaving the fresh stacks in foil on the shelf is a lot easier than that ime. you can just grab out however many petris you need & then gently roll the foil back up over the remaining plates


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse] * 1
    #28577917 -

Oh man, wish I'd bumped into you a couple weeks ago! Would've saved me a lot of failed attempts and frustrations! I'm new to this community, and online communities in general. So as a slightly selfish noobie I just started off creating new posts very specific to my problems.. I now see how it benefits everybody to dive into existing threads and participate there..

Awesome glass Petri dish thread there man. Saved to my favourites. So I got the painter's tape idea from a small YouTuber, very nice guy working big batches, with his own ways. But yeah tape isn't for me. That being said he introduced me to no-pour and glass petris in general. I wouldn't see myself using anything disposable (at my very modest scale anyway), and PP is very hard to find where I live, importing it from the US triples its price, and it goes cloudy anyway.. So glass it is. Love the feel and handling too, washing is a bit of a pita but with time I'm getting faster at it.

One big con I found to glass petris at first is the fact the lid is as high as the container. Meaning you have to seal at an angle rather than nicely along the side.. but I guess you get used to it. Not a problem for you?

Thanks for the super detailed description of drying out your plates!! So if I understand correctly, SAB means unwrapping and drying stacked, which can be risky - I've had a stack collapse more than once. And on the shelf means leaving stacked in original wrap, taking them out as needed and voila. The latter seems way easier, gonna do that.

The only thing I'd have to be super careful with then is keeping the dish closed at all times (tight between my fingers) as I pull it ouf the foil and put into my SAB right? Seems to be a thing with glass dishes, handling them tight and closed at all times when they're not sealed. Love the idea of not having to seal them until necessary though.

As for my weird bacteria under agar.. it occurs on the destination plates, not the from plates. So I'd transfer something (myc, fruit sample, grain) from a plate into a new pristine plate. Which has dried for a few days. Next day, I see a blotch of bacteria in the destination plate, completely under the agar, and it then spreads to the whole surface over the next week or so, but still remaining under the agar. Is it possible that a transferred contam would slide itself under the agar, and stay under only? Really weird.. When cleaning those plates, I looked and scraped on top and under to check, and this specific bacteria (looks like a water mark) definitely lives between the underside of the agar and the container glass. Note -> that I was using pour technique, and sterilising my dishes in my microwave :ashamed: but that still doesn't explain the underneath contam thing being triggered only after inoc.

A mystery, but which I'm hoping not to run into anymore now I have great advice on both no-pour, and oven sterilisation techniques if I want to pour :yaysugar:

PS: just read through every word of your awesome glass petri thread, thanks man, really helpful, I'll post questions/comments directly in there.


--------------------


Reaching for the summit, one way or another :leaving:



Edited by starburstmemories (12/10/23 10:52 PM)

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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28577983 -

hahaha i think a lot of people start out that way on this site. it can be a lot to learn & get used to, especially if you aren't as familiar with older style forum boards.

hell yeah, glad you found it helpful! i wanted to make it as approachable & laid out as possible. everyone's got different methods they prefer but we all gotta use what makes the most sense for our situation. washing them does suck but it's a pretty small con for having them be super clear & reusable

yeah i don't have an issue wrapping them. one i inoculate them or am gonna have them in storage loose on the shelf i just use a 1.5ish roll of cling wrap. just pull it tight around the outer edge & you can kinda smoosh it flat around the bottom, so about half the strip is around the edge & the other half is over the gap between the plates. awkward to describe but easy to do once you get used to it

exactly yes. can be an incredibly precarious situation when they are stacked without any cling wrap/parafilm around them. once you have them wrapped they stack fine, but unwrapped glass is slippery. easy to fuck up & bump the SAB. i had a stack of 3 fall off my rack because my flowhood was vibrating the table lol. the foil/making a container & just taking them out as needed has worked well for me

yeah if you're really careful with handling, they should stay clean while unwrapped. i just personally don't wanna risk me fucking up because it's easy to bump a lid, so storing in the foil/a container is nice. i'd just bring the foil stack into the SAB & unwrap it there, that way you don't need to worry about transporting it

hmm yeah that's weird. maybe the plates just weren't fully sterilized properly & the timing of it happening with the transfers is coincidental. or maybe by transferring you're cutting to the bottom & introducing something there. idk no idea really lol but hopefully the no pour method can solve that for ya! if not you'll have to do some detective work. but yeah, glad the write up helped out. my lines always open!


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28579385 -

Haha man... it actually happened no later than today. I bumped my sab ever so slightly and a stack of 6 came down like a landslide :migraine:

I think it might be ok though, these things are so slippery and heavy they remained shut all along. Time will tell!

As for my mysterious intruder, I think you're right, dishes not 100% sterile due to using a shady MW method. But the correlation is there, what I think it is is during inoc some kind of environmental change (shock, temp) triggered that ever so subtle pre-existing contam..

Thing of the past, and I'll move on to your epic thread for anything glass dishes. Thanks again man, your patience and generosity is legendary!


--------------------


Reaching for the summit, one way or another :leaving:



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