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Re: Glass Petri Agar Tek [Re: LotKid]
    #28058794 -

LotKid said:
Cling wrap is for the kitchen. Parafilm is for the lab. I've never had that problem with parafilm.

#ParafilmGang


I am just starting out and have both parafilm that I haven't used yet and Saran wrap which I have.  I do my work in a virtually unused bathroom downstairs.  It's still, quiet and I can keep it really clean with little effort.  But it ain't a lab.  Decisions decisions :laugh:

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Re: Glass Petri Agar Tek [Re: sandman420]
    #28058909 -

Try both, see which you like best. My money on cling wrap


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Currently looking for nothing. You guys who sent me stuff are straight up awesome!. :mushroom2:

We don't own things - things own us.

Semi-solid liquid culture (SSLC)

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Re: Glass Petri Agar Tek [Re: Rusty2096] * 4
    #28059054 -

Cling wrap is for keeping leftover uncle ben's fresher longer... :laugh2:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid] * 1
    #28059070 -

Ironically reading about the infamous Uncle Bens "tek" brought me here.  Thankfully I ate the agar pill and went that direction. Thanks to you all.

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Re: Glass Petri Agar Tek [Re: MycOnABike] * 3
    #28059717 -



:lmafo:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Agar Tek [Re: LotKid]
    #28076703 -

Thanks for the tek, I've had endless contaminations by way of pressure cooking to sterilise dishes.

I assume a lot of my contamination vectors came from the dishes being wet on the outside (possibly facilitating bacterial contamination), vacuumed and very cumbersome to deal with. I look forward to trying out using an oven for sterilisation instead.

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Re: Glass Petri Agar Tek [Re: Effigy]
    #28164872 -

I just found this post after trying a no-pour with glass dishes in stainless sleaves. It was a total shit show. Everything is so wet! SO WET!

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Re: Glass Petri Agar Tek [Re: sandman420]
    #28576454 -

Thanks man, been looking for this thread for the past 3 weeks! Read it all, I feel enlightened :tongue2:

Sorry you had to put up with this hater, but hey it's kinda like having your own stalker... in a way it's a compliment!

So I'm a beginner and spent the past 3 weeks trying all kinds of teks. I use a sab and glass dishes. Best result I got contam wise was no-pour, but too messy and condensation was wild.

I then came across scientific papers and forums that swear you can use your microwave, both for dishes and agar. So I did. Over and over. Dropped MWing agar, for which I went back to PC, but kept using it for dishes cause they come out dry and clean.. and every time I think it's all good, I end up disappointed.

So does everybody on shroomery agree that microwaves are BS in all possible ways? Sorry I'm a noob, and the convenience and cleanliness were attractive...

The weird thing is that the best results I thought I got looked promising. Super clan and condensation free plates, poured in my sab, pristine after 4 days. But as soon as I inoculate them, next day I get this bacteria spreading UNDER the agar!  It's been happening pretty systematically. You'd think if under, it should have been there from the start (pre inoc), and I don't understand how inoculating on top of the agar can trigger bacteria underneath.. I have this little gif that shows parallax between the bacteria and myc. I also checked physically and it's definitely underneath...

I'll drop microwaving anything right now that I found this thread, but just curious if anyone has an idea what happened there?

Going oven right now.. the only thing is it does seem like a waste heating up my big oven for the small batches I make (5 to 10).. maybe will look into buying that roasting oven as mentioned. And hopefully won't trigger another wave of haters!!! :laugh2:


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Reaching for the summit, one way or another :leaving:



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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28576526 -

no pour glass petris usually dry out pretty well if you have the right containers or leave them on the shelf in foil long enough. you can let them dry out in an SAB for a day or two as well. might be worth trying no pours again if you can’t get pouring them to be consistent

sounds like your dishes are somehow getting bacteria in them at some point between sterilization & pouring. maybe your hands hover or pass over the dish in a way that allows bacteria to fall in while you’re handling them or pouring. i don’t know anything about microwaving agar but as far as im aware it is not at all common practice for us here & i wouldn’t recommend you do it when the PC is so easy & a guaranteed way to make something sterile. it could be some part of your technique or sterilization process but there’s no reason to add the microwave into the mix of possible contamination vectors


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28576642 -

I see where I went wrong with no-pour, thank you! I let my stack cool down and then stuck it in the fridge, which I guess trapped the condensation in… I also used wide painter’s tape, but you mention foil. Is that the same foil method as the oven one? As in rolling your stack in foil?

When you say to let them dry out in my sab, is that the whole stack still in foil or individually?

Yes I see what you’re saying about bringing in contam during handling or pouring. What puzzles me is that my plates stay clean for several days while I let them dry in my sab.. The bacteria under the agar only starts showing right after inoculation (less than a day), if it was there all along shouldn’t it have started spreading during the previous drying days?

Also during those drying/testing days I let them be as is stacked, no sealing. Should I seal them as soon as they’ve dried up instead?


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Reaching for the summit, one way or another :leaving:



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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28577024 -

oh yeah man the fridge is a nightmare for condensation lol there's no reason to keep blank plates in the fridge ever imo. they sit just fine on the shelf. no idea where you were using painters tape but yeah i wrap my no pours in foil just like you would for the oven, except there's agar in them & they go in the PC. the glass petri link in my sig is how i do them from start to finish if you need more details

i would unwrap them in stacks if you were gonna dry them in the SAB. they should be fine to sit in the foil on the shelf for awhile. i've had them dry out pretty well just chillin on the shelf but it might take a few days or more before they really get dry that way. last time i did a foil wrap, the foil was juuuust short enough to leave the top plate exposed on the top so you could see down into it as if you were using it & i think that helped quite a bit with drying out. the foil wrap generally just has to be sturdy enough to keep them from falling over, doesn't have to be perfectly sealed or anything

yeah i see what you're saying, that is weird. it's possible the timing is just super coincidental but it does seem correlated to you inoculating them. maybe do some blank transfers to see if the problem still pops up, & also leave some plates completely blank as a control? if it's not in there before you transfer it must be getting brought in when you do. is the bacteria right under where you drag your scalpel? or does it end up all over/on random parts of the plate?

if they stay in your SAB they should be fine to stay unwrapped, but if they are coming out of your SAB at any point i'd wrap them. i've done some limited testing with unwrapped glass & they don't seem to NEED to be wrapped, but handling them properly can be really tricky without a strip of cling wrap/parafilm around the bottom edge. if you have stacks of petris you won't use for awhile you can wrap them in a full sheet of seran wrap & cut off the petris you wanna use. but leaving the fresh stacks in foil on the shelf is a lot easier than that ime. you can just grab out however many petris you need & then gently roll the foil back up over the remaining plates


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Glass Petri Agar Tek [Re: hazyhorse] * 1
    #28577917 -

Oh man, wish I'd bumped into you a couple weeks ago! Would've saved me a lot of failed attempts and frustrations! I'm new to this community, and online communities in general. So as a slightly selfish noobie I just started off creating new posts very specific to my problems.. I now see how it benefits everybody to dive into existing threads and participate there..

Awesome glass Petri dish thread there man. Saved to my favourites. So I got the painter's tape idea from a small YouTuber, very nice guy working big batches, with his own ways. But yeah tape isn't for me. That being said he introduced me to no-pour and glass petris in general. I wouldn't see myself using anything disposable (at my very modest scale anyway), and PP is very hard to find where I live, importing it from the US triples its price, and it goes cloudy anyway.. So glass it is. Love the feel and handling too, washing is a bit of a pita but with time I'm getting faster at it.

One big con I found to glass petris at first is the fact the lid is as high as the container. Meaning you have to seal at an angle rather than nicely along the side.. but I guess you get used to it. Not a problem for you?

Thanks for the super detailed description of drying out your plates!! So if I understand correctly, SAB means unwrapping and drying stacked, which can be risky - I've had a stack collapse more than once. And on the shelf means leaving stacked in original wrap, taking them out as needed and voila. The latter seems way easier, gonna do that.

The only thing I'd have to be super careful with then is keeping the dish closed at all times (tight between my fingers) as I pull it ouf the foil and put into my SAB right? Seems to be a thing with glass dishes, handling them tight and closed at all times when they're not sealed. Love the idea of not having to seal them until necessary though.

As for my weird bacteria under agar.. it occurs on the destination plates, not the from plates. So I'd transfer something (myc, fruit sample, grain) from a plate into a new pristine plate. Which has dried for a few days. Next day, I see a blotch of bacteria in the destination plate, completely under the agar, and it then spreads to the whole surface over the next week or so, but still remaining under the agar. Is it possible that a transferred contam would slide itself under the agar, and stay under only? Really weird.. When cleaning those plates, I looked and scraped on top and under to check, and this specific bacteria (looks like a water mark) definitely lives between the underside of the agar and the container glass. Note -> that I was using pour technique, and sterilising my dishes in my microwave :ashamed: but that still doesn't explain the underneath contam thing being triggered only after inoc.

A mystery, but which I'm hoping not to run into anymore now I have great advice on both no-pour, and oven sterilisation techniques if I want to pour :yaysugar:

PS: just read through every word of your awesome glass petri thread, thanks man, really helpful, I'll post questions/comments directly in there.


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Reaching for the summit, one way or another :leaving:



Edited by starburstmemories (12/10/23 10:52 PM)

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Re: Glass Petri Agar Tek [Re: starburstmemories]
    #28577983 -

hahaha i think a lot of people start out that way on this site. it can be a lot to learn & get used to, especially if you aren't as familiar with older style forum boards.

hell yeah, glad you found it helpful! i wanted to make it as approachable & laid out as possible. everyone's got different methods they prefer but we all gotta use what makes the most sense for our situation. washing them does suck but it's a pretty small con for having them be super clear & reusable

yeah i don't have an issue wrapping them. one i inoculate them or am gonna have them in storage loose on the shelf i just use a 1.5ish roll of cling wrap. just pull it tight around the outer edge & you can kinda smoosh it flat around the bottom, so about half the strip is around the edge & the other half is over the gap between the plates. awkward to describe but easy to do once you get used to it

exactly yes. can be an incredibly precarious situation when they are stacked without any cling wrap/parafilm around them. once you have them wrapped they stack fine, but unwrapped glass is slippery. easy to fuck up & bump the SAB. i had a stack of 3 fall off my rack because my flowhood was vibrating the table lol. the foil/making a container & just taking them out as needed has worked well for me

yeah if you're really careful with handling, they should stay clean while unwrapped. i just personally don't wanna risk me fucking up because it's easy to bump a lid, so storing in the foil/a container is nice. i'd just bring the foil stack into the SAB & unwrap it there, that way you don't need to worry about transporting it

hmm yeah that's weird. maybe the plates just weren't fully sterilized properly & the timing of it happening with the transfers is coincidental. or maybe by transferring you're cutting to the bottom & introducing something there. idk no idea really lol but hopefully the no pour method can solve that for ya! if not you'll have to do some detective work. but yeah, glad the write up helped out. my lines always open!


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: Glass Petri Agar Tek [Re: hazyhorse]
    #28579385 -

Haha man... it actually happened no later than today. I bumped my sab ever so slightly and a stack of 6 came down like a landslide :migraine:

I think it might be ok though, these things are so slippery and heavy they remained shut all along. Time will tell!

As for my mysterious intruder, I think you're right, dishes not 100% sterile due to using a shady MW method. But the correlation is there, what I think it is is during inoc some kind of environmental change (shock, temp) triggered that ever so subtle pre-existing contam..

Thing of the past, and I'll move on to your epic thread for anything glass dishes. Thanks again man, your patience and generosity is legendary!


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Reaching for the summit, one way or another :leaving:



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