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Offlinesparks
addict
Registered: 03/01/01
Posts: 55
Last seen: 22 years, 3 months
Re: Peroxidated Agar Tek * 1
    #66586 - 10/14/99 12:38 AM (23 years, 11 months ago)

Thanks for the info.
I have had my share of contaminated agar cultures, and it has made me look for other ways to clone mushrooms, but maybe agar is the way to go anyway.


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Invisiblemycofile
Pooh-Bah
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Registered: 01/18/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Peroxidated Agar Tek [Re: sparks]
    #66588 - 10/14/99 01:16 AM (23 years, 11 months ago)

Word!
I like it!
It excites me!
It makes me feel stupid, why didn't I think of it?
Experiment #1 begining in 5 min.
1 jar peroxidated agar will be left out. Open! Only a paper towel (sterilized with the jar) will cover it, to prevent germinated colonies from landing.
I wonder how long it will take the peroxide to break down? Anybody wanna guess?
Oh yeah, I'll do one non peroxide, just to laugh at it.
Good post!

------------------
"...From a certain point of view..."
-Jedi Master Obi-Wan Kenobi



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: Peroxidated Agar Tek [Re: sparks]
    #66589 - 10/31/99 07:00 PM (23 years, 10 months ago)

Hello,
I was wondering, does the 20 to 1 ratio of water to peroxide refer to the 3% peroxide, or does it mean 20 parts water to 1 part pure peroxide, I'm guessing that it's the 3% as the other would be like 5%, right and too strong.
thanks,
Oleander

------------------
Do you answer questions, or question answers?



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Invisiblemycofile
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Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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Re: Peroxidated Agar Tek [Re: sparks]
    #66590 - 11/01/99 02:57 AM (23 years, 10 months ago)

Yep, 20/1 water/3%H2O2. BTW, that peroxide jar of agar I left unsealed never contamed. Not at all. Actually, it is now dry and shrively, but it never grew the nasties. see, this shit works.
MHHOC

------------------
I can't go on talking like this. I just had a mental picture of Angel....
Now I'm speechless.



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Offlinejoee
journeyman
Registered: 04/06/99
Posts: 15
Last seen: 22 years, 6 months
Re: Peroxidated Agar Tek [Re: sparks]
    #66591 - 11/23/99 12:42 AM (23 years, 10 months ago)

bump-
anyone else have sucess?
if one were to use canning jars, which lid should he use?
also- can the plastic lids be autoclaved?


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Invisiblemycofile
Pooh-Bah
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Registered: 01/18/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Peroxidated Agar Tek [Re: sparks]
    #66592 - 11/23/99 03:43 PM (23 years, 10 months ago)

I recomend wide-mouth 1/2 pints for jars, stock lids are fine, plastic ones can be autoclaved (pressure cooked). Got over 8 strains/species growing fine on this stuff right now.

------------------
-From a registered Mad Scientist

"From a certain point of view"
-Jedi Master Obiwan Kenobi (also a Mad Scientist tm)



--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Anonymous

Re: Peroxidated Agar Tek [Re: sparks]
    #66593 - 05/08/00 12:43 AM (23 years, 4 months ago)


Nice tek. If this idea didn't make me very happy, I'd be a total idiot. Well written bro....



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