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Offlineshroomeritus
journeyman
Registered: 12/17/00
Posts: 36
Last seen: 21 years, 5 months
Questions about using Agar!
    #323771 - 05/22/01 09:39 PM (23 years, 3 months ago)

Sup everyone =),
Well...i've finally moved on to agar. so far it's been pretty fun and i'm somewhat getting the hang of it. I just have a few questions:

1) I'm using glass petris that have no lids and i just cover the top with regualr saran wrap. Problem w/ this is that there are almost always contams along the edges and they eventually soak into the middle. Any one else use glass petris w/o lids..and any helpful suggestions?

2) Are contams normal in a petri?

3) Does the use of peroxide weaken a strain and it's contam. defense?

Thanks everyone =) I have a dish that has just germinate and no signs of contams so far =). I'm planning on making some peroxide petris to transfer this onto .





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-the sweet is never as sweet without the sour

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OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,167
Loc: my room
Last seen: 1 month, 1 day
Re: Questions about using Agar! [Re: shroomeritus]
    #323842 - 05/22/01 11:19 PM (23 years, 3 months ago)

1. Man, you need lids. Buy some.
2. if you don?t use a liminar flow hood or peroxide and if your place is "normally" clean(dirty), then contaminations are quite common,yes.
3. don?t know, I would say it doesn?t weaken the strain, but a prolonged use could bring some contamination with it.

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kickme.to/mushrooms

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Offlinesylo
addict
Registered: 10/29/00
Posts: 219
Last seen: 22 years, 4 months
Re: Questions about using Agar! [Re: shroomeritus]
    #323877 - 05/22/01 11:56 PM (23 years, 3 months ago)

I prefer glass bottles with flat sides to petries. They are a little harder to work with but they do not dry out or contam so easily.


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