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Anonymous

Re: Antibiotic Agar Mix for Spore Germiniation in Water?
    #16265 - 05/10/00 02:28 AM (22 years, 4 months ago)

You should be fine, anitbiotic means it kills/reduces bacteria. You will still have problems with molds and such because they will germinate just like mushie spores will. You should try and use a HEPA glove box or flow hood if you are not using H2O2 in your agar.

If you have several jars of mycelium going, You can take a chunk of one (only a few strands is needed) and place on a perixide/agar plate and go from there. Just remember not to go from plate to plate to plate for ever. You need to start with fresh cultures now and then or you will have a problem with senescence (strain degeneration and loss of viability)

For the peroxide/agar info just do a search for Placebo or chestercopperpott and you will find good info.

------------------
-ChesterCopperpott-
"Old enough to know better, young enough to do it anyway."



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Anonymous

Re: Antibiotic Agar Mix for Spore Germiniation in Water?
    #16267 - 05/10/00 03:46 AM (22 years, 4 months ago)

Thanks Chester,

I have read your agar/peroxide tek and may try it also, but I am really talking about the liquid mycelium tek which calls for a 4% by weight malt mixed in water and sterilized for a growth medium.

I have some honey jars going now, about 4 or 5 days with something growing not sure what!?!

So, thanks for your help



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