Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags, Substrate Mix

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineiudexk
member
Registered: 08/18/01
Posts: 107
Loc: UK
Last seen: 22 years, 3 months
agar/peroxide conc.
    #379912 - 08/27/01 11:46 AM (22 years, 7 months ago)

Is this correct?:

50g antibiotic MEA
make up to 950mL w/dH2O
add 5mL 6% H2O2
make up to 1L w/dH2O

Would this then be safe to innoculate w/spores if poured into a sterile petri dish? Or would I have to steam/sterilise first?

Many thanks,
IudexK

http://www.magickherbs.co.uk/


--------------------
http://www.magickherbs.co.uk/

Extras: Filter Print Post Top
Offlinejonnyshaggs420
Pooh-Bah

Registered: 08/08/00
Posts: 1,965
Loc: Mid-West
Last seen: 18 years, 6 months
Re: agar/peroxide conc. [Re: iudexk] * 1
    #379965 - 08/27/01 01:17 PM (22 years, 7 months ago)

Heres an equation for you:

Spores + H2O2= DEAD SPORES

You can use peroxidated agar for transfers, but it will kill all and any spores that come in contact with it. And you might want to mix up the agar, sterilize, then add the peroxide. Because peroxide is degraded significantly by heat among other things.

Novelty


--------------------
Vote Jonnyshaggs in the next election for GOD...Its the responsible choice

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Substrate Bags, Substrate Mix


Similar ThreadsPosterViewsRepliesLast post
* Agar & Peroxide Question oddiseus 1,592 7 02/22/05 04:25 PM
by oddiseus
* Re: Plate Count Agar? Is this ready to use? shroomeritus 2,062 8 04/24/01 07:53 PM
by shroomeritus
* Peroxide in the Perlite? Retna 1,646 8 04/16/04 05:36 PM
by TheDrugStore
* Re: Agar w/ peroxide info Hippie3 1,618 2 05/07/00 02:20 AM
by mycofile
* h2o2 question
( 1 2 all )
MushyMan 2,531 30 04/03/03 09:59 AM
by dog
* Re: Where can one find H2O2??? Anonymous 906 4 06/16/00 01:55 PM
by tom
* some questions about agar buggers 1,729 15 08/27/05 07:25 AM
by buggers
* Re: Antibiotic Agar Mix for Spore Germiniation in Water? Anonymous 1,534 1 05/09/00 06:46 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,337 topic views. 19 members, 127 guests and 18 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.008 seconds on 14 queries.