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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #27125597 -

I’m still pretty new to all this. I have a ton agar plates on the go. I’m getting better at sectoring:) I tried MycoWillys fauxhood(box fan and furnace filter) Bad idea most contam I’ve had on agar. Mind you the fauxhood makes the garage smell cleaner. SAB is the way to go! I stumbled across this link on mycelium growth and contam and figured I’d share for the other noobs!
https://www.sciencebuddies.org/science-fair-projects/references/interpreting-agar-plates

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Steve9333]
    #27125621 -

Steve9333 said:
I tried MycoWillys fauxhood(box fan and furnace filter) Bad idea most contam I’ve had on agar.



Sounds about right.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: jay.ach]
    #27125638 -

jay.ach said:
Purple Mystic T-5





All of my transfers for Purple Mystic have been similar to this one from my T-5 group.  About one-third of each transfer is very slow. In these shots, it's the 4 o'clock to  7 o'clock slice.  It eventually fills in but I assume this means I'm transferring something bad along with myc. 

I'm taking my transfers from the opposite side of the slow growth.  I'm planning on continuing to transfer until I get consistent growth.  Any other suggestions?



honestly im interested too. what could be the culrpit here? I don't see anything suspicious on your myc though.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phony Phone]
    #27125919 -

How would you be able to see anything on the mycelium without a microscope

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft]
    #27125948 -

Id wager that the side with the strongest growth is what would've been towards the inside of your punch... we talk of taking the leading edge, but the back half of that leading edge tends to have stronger myc than the actual leading edge.

Next time you take transfers, note which direction you place the "true" leading edge of your xfer. Ill bet ya a pack of spore prints the opposite side of the leading edge will grow stronger.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126035 -

That's an interesting observation, do you take your xfers slightly back from the edge then?


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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #27126235 -

I just noticed it more and more from when I take bigger chunks as transfers... the closer-to-the-center segments always took off faster and almost always were more rhizo... ill see if I can dig up some pics to illustrate my point...

Edit:

You can kinda see what I mean here... the 6 o'clock area was the "leading edge" from the donor plate... the 12 o'clock area would've been closer to the center of the parent plate... I believe the more established myc from the interior of the parent plate tends to be stronger... the whole leading edge idea is just to ensure you're not transferring something insidious.

Edited by roarkell (01/04/21 04:02 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126248 -


Toc grain spawn to agar
I like how it fills out the bubble, looked neat super close up.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Quadryder]
    #27126283 -

What if you take a few transfers and stack them all on top of each other. Wouldn’t that cut down on Petris?


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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126323 -

I'll have to keep an eye for that, it could be useful to know when transferring something more sensitive like pan myc where the selection process is more vital


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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #27126382 -



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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #27126412 -

All my prints are 4-7 years old. I’m trying to get them started again. The first ten plates took a long time to show growth and when it did it was extremely tomentose and I had a hard time deciding if it was all contam. I didn’t have any odd color it just all looked more like mold. I made some more plates and used another old print on most of them but saved three to make transfers off the best first plates.
When i was cutting transfers they were so whispy that the residual alcohol on my scalpel made it appear to dissolve to nothing. I transferred anyway and a week later the transfers look a lot thicker than the growth They came from yet not quite rhisomorphic. I understand the rate of growth can have something to do with the agar mixture but I’m curious if anyone out there has had a similar experience bringing back a variety that has been a print for a very long time. If people are interested I can take some pics of what I’m seeing


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Re: AGAR ENVY! (Anything and All things agar!) [Re: NOS4A2] * 1
    #27126417 -

Pics or it didn’t happen :awedance:


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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale]
    #27126436 -

Lol ok when i get out of work


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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126442 -

roarkell said:
I just noticed it more and more from when I take bigger chunks as transfers... the closer-to-the-center segments always took off faster and almost always were more rhizo... ill see if I can dig up some pics to illustrate my point...

Edit:

You can kinda see what I mean here... the 6 o'clock area was the "leading edge" from the donor plate... the 12 o'clock area would've been closer to the center of the parent plate... I believe the more established myc from the interior of the parent plate tends to be stronger... the whole leading edge idea is just to ensure you're not transferring something insidious.



I have noticed this before on my plates but was not sure how to explain it.
My mind came up with the assumption that there were other genetics in play that were growing faster than others on the transfer (which is usually the case)
However looking back at my earlier plates I can tell there are many that I took at the leading edge which recovered slower than the growth behind it.
I appreciate the enlightenment Roarkell.

Edited by mushhead (01/04/21 05:53 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead]
    #27126454 -

Each strain present grows at slightly different rates right? If you isolate a faster strain it may fruit faster, but if the yield or potency was initially carried in genetics that were excluded then your culture may not be ideal just because it's fast. Leading edge is fast, but is it robust?


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Never give up, never surrender.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #27126540 -

Quirkmeister92 said:
Each strain present grows at slightly different rates right? If you isolate a faster strain it may fruit faster, but if the yield or potency was initially carried in genetics that were excluded then your culture may not be ideal just because it's fast. Leading edge is fast, but is it robust?



Exactly... im looking for overall strength... speed helps, but I'd rather have great fruits that take an extra day or two...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126584 -

I mean lets say those variable aren't present, the culture you're working with is in fact potent, clean and speedy(within reason).
What roarkell brought to my understanding was that if you were to take from closer to the middle and not the edge it could help with faster recovery of your xfer.
Going over my pics of transfers from the start of 2020 I have noticed that on multiple occasions where my xfer recovered slowly as it was the direct leading edge of the growth.
Not that slow recovery is anything I'm too worried over, but if xfering a couple centimeters closer to the middle of your plate helps with recovery? Damn. Wish I had known.
I've got a couple cultures I am going to re-evaluate because of this tiny little piece of info..

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead]
    #27126601 -

I dont mean to suggest that you shouldn't take from the leading edge... theres still a fair chance your myc could've crawled right over something nasty... all im saying is the side that isn't "leading" tends to recover better because its more established.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27126618 -

Interesting I’ve never paid attention but I will now.


I was always told you take the leading edge because that’s the newest most active myc growing and looking for food.


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LAGM2020

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