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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27118019 -

What's everyones input on getting swabs to germinate?

Streak a few plates and then bury the swab in the last?

Use scissors or tweezers to cut/pull small amounts of the cotton tips off and drop onto multiple plates?

I've got so many great varieties now on swabs and i'm paranoid because only having two swabs of each variety means i've only got a handful of chances to get something viable before i've lost the variety entirely.

Would love to hear from several people on their best practice for using swabs as long as possible.

Edited by Mr. Mushie (12/31/20 12:54 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27118037 -

Long answer short. Yes, why not do all those? Streak 2, rip a few pieces off and dunk that bish


--------------------

D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27118240 -

Mr. Mushie,

On 12/27, I tried my first swabs.  I streaked one plate with one swab of Ban Phang Ka and jammed the tip of the other BPK swab into another plate.


Today on 12/31, I still don't see much happening.






Good luck to you!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tomcards]
    #27118346 -

The best luck I’ve had with swabs was by tearing small filament off with your sterilized scalpel and putting that into the agar.

No luck with streaks


--------------------
"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #27118433 -

Any of you guys have an idea what is this transparent growth on the leading edge of mycelium on agar?



And this one:

The red circled area was transparent before it filled in but it's different looking to the opposite side where the mycelium is white and flows.
The red arrows show the transparent growth.

I could see the rhizo structures form on the transparent part before it filled in as white mycelium. What is it?
Note: These plates have slightly lower nutes than the ones that the transfers came from.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27119273 -

D3monic said:
Long answer short. Yes, why not do all those? Streak 2, rip a few pieces off and dunk that bish


I appreciate the input D3, Tom, and Rafiikii.

I wish they weren't so tricky to get germination from, i'm quite stressed about it actually.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27120171 -

Thought about using some beet juice to replace dye and dex. Anyone tried this?


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale]
    #27120233 -

Rafiikii said:
The best luck I’ve had with swabs was by tearing small filament off with your sterilized scalpel and putting that into the agar.

No luck with streaks


wish I'd seen this before I went and jammed mine in the agar lol good idea. I'll try that with my reserve yeti swab if the others' doesn't take.


Adrenochrome said:
Thought about using some beet juice to replace dye and dex. Anyone tried this?


juice from fresh beets mixed with water and agar? I'm not sure the nutrient profile of beets but I'm pretty sure they don't have any starch so you might need to add LME. They'd make a really cool and effective dye though, I've always liked their color. My grandma makes a mean beet soup. Might whip up a pot and see if I can't get some beet water first when I boil them


--------------------
No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
:sunny:Photosynthesis: A Morning Ritual:sunny:
Ballzagna's Tek Compendium and Notes for Noobs



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ballzagna]
    #27120238 -

Hell yeah. That’s what I’m sayin. Maybe not a nute substitute, but just a for funsies additive


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ballzagna]
    #27120240 -

Beets are full of sugar


--------------------

Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Just_A_Noob]
    #27120244 -

Yeah, so to replace dextrose and give it color. Still use potatoes or brf


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Eugene Gesuale]
    #27120412 -

Adrenochrome said:
Thought about using some beet juice to replace dye and dex. Anyone tried this?


There are some threads about it, and some posts in this thread.  It'll grow myc but the color fades quite a bit during the PC cycle so you won't get beet red agar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: adhoc]
    #27120413 -

Good to know


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27120665 -

Mr. Mushie said:
D3monic said:
Long answer short. Yes, why not do all those? Streak 2, rip a few pieces off and dunk that bish


I appreciate the input D3, Tom, and Rafiikii.

I wish they weren't so tricky to get germination from, i'm quite stressed about it actually.



Mr. Mushie,


On 12/31, 4 days after inoculation, mycelium appeared on the tip plate.

Today, on 1/1/21, 5 days after inoculation, mycelium appeared on the streaked plate.

The tip germinated quicker and its mycelium is growing more robustly than the streak.





In 1974, streaking naked was the rage for about 4 months.  At the tender age of 14, I streaked around the block near my house, because it was THE THING to do at the time. It was an embarrassing, ungratifying moment for me and a scandalous, repellent event for my unfortunate neighbors.  I never streaked again.

I will never streak another plate with a swab.


Today I also inoculated two plates with two Melmac swabs.

I used a sterilized scalpel to cut off the spore-laden portion of the swabs.  I wasn't able to remove the severed sections with the scalpel, so I used sterilized scissors to cut off the sections and place them on plates.





What a mess!  Fibers everywhere, resulting in multiple inoculation sites. I look forward to cleaning these plates.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tomcards]
    #27120740 -

tomcards said:
Mr. Mushie said:
D3monic said:
Long answer short. Yes, why not do all those? Streak 2, rip a few pieces off and dunk that bish


I appreciate the input D3, Tom, and Rafiikii.

I wish they weren't so tricky to get germination from, i'm quite stressed about it actually.



Mr. Mushie,


On 12/31, 4 days after inoculation, mycelium appeared on the tip plate.

Today, on 1/1/21, 5 days after inoculation, mycelium appeared on the streaked plate.

The tip germinated quicker and its mycelium is growing more robustly than the streak.





In 1974, streaking naked was the rage for about 4 months.  At the tender age of 14, I streaked around the block near my house, because it was THE THING to do at the time. It was an embarrassing, ungratifying moment for me and a scandalous, repellent event for my unfortunate neighbors.  I never streaked again.

I will never streak another plate with a swab.


Today I also inoculated two plates with two Melmac swabs.

I used a sterilized scalpel to cut off the spore-laden portion of the swabs.  I wasn't able to remove the severed sections with the scalpel, so I used sterilized scissors to cut off the sections and place them on plates.





What a mess!  Fibers everywhere, resulting in multiple inoculation sites. I look forward to cleaning these plates.


All of the swabs I keep receiving come broken upon arrival, so it's not like I can streak plates even if I wanted to. :shrug:



Going to get some long tweezers so that I can pull the fibers and stick them into the agar in the future.

Edited by Mr. Mushie (01/01/21 06:41 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27120766 -

lol yea that sucks, there hasn't been a single swab with a wood stick that I didn't receive broken. There are medical swabs with the sticks made of other materials that won't break so easily.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27120776 -

Mr. Mushie said:
tomcards said:
Mr. Mushie said:
D3monic said:
Long answer short. Yes, why not do all those? Streak 2, rip a few pieces off and dunk that bish


I appreciate the input D3, Tom, and Rafiikii.

I wish they weren't so tricky to get germination from, i'm quite stressed about it actually.



Mr. Mushie,


On 12/31, 4 days after inoculation, mycelium appeared on the tip plate.

Today, on 1/1/21, 5 days after inoculation, mycelium appeared on the streaked plate.

The tip germinated quicker and its mycelium is growing more robustly than the streak.





In 1974, streaking naked was the rage for about 4 months.  At the tender age of 14, I streaked around the block near my house, because it was THE THING to do at the time. It was an embarrassing, ungratifying moment for me and a scandalous, repellent event for my unfortunate neighbors.  I never streaked again.

I will never streak another plate with a swab.


Today I also inoculated two plates with two Melmac swabs.

I used a sterilized scalpel to cut off the spore-laden portion of the swabs.  I wasn't able to remove the severed sections with the scalpel, so I used sterilized scissors to cut off the sections and place them on plates.





What a mess!  Fibers everywhere, resulting in multiple inoculation sites. I look forward to cleaning these plates.


All of the swabs I keep receiving come broken upon arrival, so it's not like I can streak plates even if I wanted to. :shrug:



Going to get some long tweezers so that I can pull the fibers and stick them into the agar in the future.


Looks like Angela's swabs


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr]
    #27120808 -

You can still use those man. Half the time I break mine in half to use them and jam it into the plate anyway.

I’m pretty surprised none of mine have shown up broken now, never even thought of that.


--------------------
LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27120833 -

Are you using tweezers to accomplish that?

If not, id imagine your germ plates resemble a rainbow.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr. Mushie]
    #27120907 -

lol no. Just hold the plate sideways while you streak it.


--------------------
LAGM2020

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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