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mushboy
modboy

Registered: 04/24/05
Posts: 40,591
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Wall.E]
#27074621 - 12/06/20 05:36 AM (5 years, 9 months ago)

That's a good indication that something is riding along with that culture.

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Making Liquid Culture : Go insane with LC
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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27074631 - 12/06/20 05:49 AM (5 years, 9 months ago)

Figured as much and that's why it's in the trash

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Mehhh

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27074632 - 12/06/20 05:50 AM (5 years, 9 months ago)

Meshed-in mold does that.

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LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27074832 - 12/06/20 09:19 AM (5 years, 9 months ago)

A.k.a said:

Looks like it’s passed with flying colors.

How did you test it? I get incredibly paranoid dipping the loop into the lc.

I did like mentioned. Just poured some into a petri lid then used the loop

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MycoJones
Stranger

Registered: 11/29/20
Posts: 18
Loc: Tropical Island
Last seen: 7 months, 27 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27075035 - 12/06/20 10:56 AM (5 years, 9 months ago)

Tried to germinate APE spore swabs on Agar without much success.
Out of 5 plates this is the only one showing some activity. Not sure if I am seeing mycelium or a contamination though. The little bright white spot seems to grow like a little mountain instead of spreading out for now.

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capntrips7
Moldsmith

Registered: 10/21/20
Posts: 91
Loc: Urithiru

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#27076440 - 12/07/20 01:42 AM (5 years, 9 months ago)

I have had 3 varieties pin (B+, GT, Tamp), so I have little doubt about what I'm growing there, but all of my AA+ plates look questionable:

What do you guys think? Any of them look worth continuing to transfer?

I should note that other than a few plates where myc outran initial contam, none of my plates have rhizo growth, so the lack here doesn't mean much. I reduced the nutes in my last batch of agar, which will hopefully help with that going forward.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: capntrips7]
#27076505 - 12/07/20 02:45 AM (5 years, 9 months ago)

plenty to work with there

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Josex
#cheat_code

Registered: 11/13/15
Posts: 8,999
Loc:

Re: AGAR ENVY! (Anything and All things agar!)

[Re: MycoJones]
#27076512 - 12/07/20 02:53 AM (5 years, 9 months ago)

MycoJones said:

Tried to germinate APE spore swabs on Agar without much success.
Out of 5 plates this is the only one showing some activity. Not sure if I am seeing mycelium or a contamination though. The little bright white spot seems to grow like a little mountain instead of spreading out for now.

For germinating stubborn APE spores from swabs I like to wet the swab in sterile water and then bury small pieces of it in PF-tek substrate, it works really well. The last link in my sig has the details, scroll down till the end.

--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

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adhoc

Registered: 05/03/20
Posts: 167
Last seen: 6 months, 26 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Josex]
#27076706 - 12/07/20 08:05 AM (5 years, 9 months ago)

My first plate that kind of looks like it's supposed to. Pic isn't the best, but could that go to grain or should I do another transfer? I think that is transfer 3.

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Eugene Gesuale
Jar-Sniffer

Registered: 04/12/20
Posts: 1,920
Loc: The Basement

Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: adhoc]

1
#27076887 - 12/07/20 10:34 AM (5 years, 9 months ago)

adhoc said:

My first plate that kind of looks like it's supposed to. Pic isn't the best, but could that go to grain or should I do another transfer? I think that is transfer 3.

If it were me, I would xfer the tip of each spoke to a small jar of grain. Then fruit each one in the simplest way possible, in vitro even. If you have more Petris I would clone each one until you find out which fruits were most potent and ascetically pleasing, pinset, size etc. then make a master jar with that once it grows out. Really so many options depending on what you are shooting for

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Sunny Skies
Cluster Head

Registered: 05/03/17
Posts: 425
Loc: my house

Re: AGAR ENVY! (Anything and All things agar!)

[Re: adhoc]

1
#27076899 - 12/07/20 10:40 AM (5 years, 9 months ago)

I would chuck that to seed and look for something to clone.Take a transfer first though just in case.

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Eugene Gesuale
Jar-Sniffer

Registered: 04/12/20
Posts: 1,920
Loc: The Basement

Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Sunny Skies]
#27077004 - 12/07/20 11:40 AM (5 years, 9 months ago)

My question is can somebody please tell me the difference between isolating a strain And stabilizing a phenotype

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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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Eugene Gesuale
Jar-Sniffer

Registered: 04/12/20
Posts: 1,920
Loc: The Basement

Last seen: 3 years, 5 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Eugene Gesuale]
#27077005 - 12/07/20 11:43 AM (5 years, 9 months ago)

For example rusty white always produces leucistic fruits from ms. How did it get to that point without ms stabilization and strain isolation and in what order

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Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.

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mushboy
modboy

Registered: 04/24/05
Posts: 40,591
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Eugene Gesuale]
#27077057 - 12/07/20 12:15 PM (5 years, 9 months ago)

Read it from the pasty himself :thumbup:

:thumbup:

https://www.shroomery.org/forums/showflat.php/Number/25284782

--------------------
:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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A.k.a
Stranger

Registered: 10/27/19
Posts: 18,222
Loc: Gaming the system
Last seen: 8 hours, 9 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27077142 - 12/07/20 01:14 PM (5 years, 9 months ago)

Yeah it didn’t just happen that shit took some doing.

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nmd_myco
Obsessed Noob

Registered: 09/05/20
Posts: 198
Loc: PNW
Last seen: 4 years, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]

1
#27077223 - 12/07/20 02:03 PM (5 years, 9 months ago)

Some invitro pins back on agar.

I only had one blank plate when I found these just barely pushing on the lid of the plate. I am going to transfer them to their own plates as I take some transfers.

side lighting is upping the macro game considerably.

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CapMeh
RX Jazzist

Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 1 year, 1 month

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nmd_myco]
#27077302 - 12/07/20 03:26 PM (5 years, 9 months ago)

What are the reasons for cloning invitro agar pins? Is it just because of the clean sample? Or does agar pinning have other implications? Like good genetics?

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mushhead
Livonia Wanderer

Registered: 08/22/14
Posts: 2,242
Loc: Dimension G-452

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]
#27077311 - 12/07/20 03:31 PM (5 years, 9 months ago)

Well there are only two reasons I take pins off my plates.
-Narrowed genetics
-Because it pinned on the agar dish it could possibly be a better pinner off substrate
However ime doing so I've run into some agar clones that are just absolutely worthless so its a gamble tbh.

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DrunkUncle

Registered: 08/09/20
Posts: 121
Loc: PNW
Last seen: 3 months, 10 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushhead]
#27077324 - 12/07/20 03:37 PM (5 years, 9 months ago)

Couple more plates. Still super fluffy growth, hoping these are all viable cultures

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OEF Combat Medic & Purple Heart Recipient

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mushboy
modboy

Registered: 04/24/05
Posts: 40,591
Loc: the woods

Re: AGAR ENVY! (Anything and All things agar!)

[Re: CapMeh]

1
#27077367 - 12/07/20 04:08 PM (5 years, 9 months ago)

CapMeh said:

clean sample? ... good genetics?

Sterile clone tissue is useful :thumbup:

:thumbup:

Agar pins usually perform nicely ime.

--------------------
:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Pages: < First | < Back | 347 | 348 | 349 | 350 | 351 | 352 | 353 | 354 | 355 | 356 | 357 | 358 | 359 | 360 | 361 | 362 | 363 | 364 | 365 | 366 | 367 | Next > | Last >

Shop:

Spores for European Microscopy

Unfolding Nature: Being in the Implicate Order

All-In-One Bags That Don't Suck

Autoflowering Cannabis Seeds

APE Liquid Culture For Sale

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