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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Infiniteshot]
    #27052952 -

  ----------->>

I was really expecting something good from these transfers, but this is the result....

___________________________________________

Tamp T2 transfers and the T1 donor plate which came from MSS germ plate.
Only 1 plate is looking iffy to me, what do you guys think?



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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27052997 -

What recipe are you using? Tamp plates look good


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #27053017 -

tryptkaloids said:
What recipe are you using? Tamp plates look good


I do 5g agar/4g LME in 250ml of water
I used to use 5g/5g. Haven’t noticed much difference in growth between the two.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: TheDoobsker]
    #27053077 -

TheDoobsker said:
  ----------->>

I was really expecting something good from these transfers, but this is the result....

___________________________________________



Interesting  :ooo:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SawCeeJack]
    #27053198 -

any tips with working with swabs?

having trouble with germination.

streaked, removed fragments, dunked the whole swab etc.

still nothing.


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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."


Edited by Rafiikii (11/22/20 10:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #27053272 -

I think I have a peu culture that looks pretty decent. Cant decide if im going to drop this one in a jar or not. Anyone have any opinions. I did pull 3 plates off of this one and they look pretty uniform. There is some light condensation on the lids and messes with the image, but for the most part pretty reasonable growth.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #27053273 -

Rafiikii said:
any tips with working with swabs?

having trouble with germination.

streaked, removed fragments, dunked the whole swab etc.

still nothing.



Sticking the whole swab into the plate worked for my APE swabs I couldn't get growth from when streaking but if that didn't work for you I think there's a tek floating around here where someone stuck the swab into the agar and then put a couple drops of sterile water on the embedded swab tip.

Might be worth trying, you should be able to find it pretty easily with the search engine.

I haven't worked with swabs enough otherwise to suggest anything else but I'm sure others know more.


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:chems:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27053284 -

Im waiting for PC to arive , but want to start some agar work, I have one litre bottel for agar, sterille petri, can i so agar? Found lots of info, people say it wont work, other it will... Is there eny real good tek?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ras_Irie]
    #27053525 -

Working on my first set of clones. I put 3 small pieces of inner stem tissue onto a couple of MEA plates. It looks like each piece is putting out new mycelium, but the growth is kind of weird looking, see the first pic. Can someone confirm this is mycelium and not some mold growing on the decomposing bits of mushroom?

My GT plate looks contamination free to me, but the PESA plate had some condensation on the lid that dripped and spread across the agar and now there are a bunch of bacterial colonies spreading across the surface. When can I try to transfer some of this myc to a new plate?



--------------------


Important Links.

Be kind to others.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lenz] * 1
    #27053552 -

Lenz said:
T3 TOC and RW plates going to grain in a few days. Dropped one of the RW plates and the center just fell out  :foreheadslap:




Also, a question: Does storing plates in the fridge completely halt growth or just slow it down extremely?



All but that last plate look sexy as fuck. Nice work


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ras_Irie]
    #27053764 -

Ras_Irie said:
Im waiting for PC to arive , but want to start some agar work, I have one litre bottel for agar, sterille petri, can i so agar? Found lots of info, people say it wont work, other it will... Is there eny real good tek?


Agar can be steam sterilized. Id let it go for an hour and a half to two


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #27053818 -

Ya know, I was super stoned pouring plates the other night (thinking about the honey porridge I was gonna have after) and I reckon I'm gonna see of I can germinate something on stodgy porridge this weekend now.
Wanna try do it without the PC.
Maybe put some in some in small jars in a double boiler type system and then whilst they're steaming put the lids on (kinda like a shitty oven tek where they rely on the hot air rising to keep out contams).
After that let them cool, let a couple go to see if anything grows and nocc up a few.
I'm bored, my cultures are clean or just transferred and all my tubs are in fruiting so why not


--------------------
Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #27053858 -

Is this pin developed enough to clone?


Here is the plate it is from:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: krock]
    #27053919 -

Yeah, clone that bitch. Myc will grow on agar no matter how small your transfer is, a young pin grows myc very fast, that'll be strong.


--------------------
Vinci luh you

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Vinci]
    #27054203 -

:whathesaid:


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27054214 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27054365 -

D3monic said:
Lenz said:
T3 TOC and RW plates going to grain in a few days. Dropped one of the RW plates and the center just fell out  :foreheadslap:




Also, a question: Does storing plates in the fridge completely halt growth or just slow it down extremely?



All but that last plate look sexy as fuck. Nice work



Thanks D3! Appreciate the comment and agar wisdom.

Had a slight hiccup with some recent plates growing mold colonies on the edges, think it might have to do with me being clumsy taking them out of Ziploc's but overall I think agar is my favorite part of cultivation.

Didn't realize how easy it is to generate nearly endless clean cultures once you get a grip on sterile technique, it's pretty awesome. :cool: Now for a flowhood....


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:chems:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #27054369 -

Got a couple pins from a Argentinian plate and the growth has been explosive with these bois.



T1 popped off faster than almost all my t7 KSSS clone, although the KSSS have cleaned up very nicely so far! trying to get that perfect even growth all around, so finicky.



--------------------
Don't be afraid of feeling the feelings
Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI

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Re: AGAR ENVY! (Anything and All things agar!) [Re: lookintolearn]
    #27054597 -

lookintolearn said:
Got a couple pins from a Argentinian plate and the growth has been explosive with these bois.



T1 popped off faster than almost all my t7 KSSS clone, although the KSSS have cleaned up very nicely so far! trying to get that perfect even growth all around, so finicky.





Beautiful looking plates. What's your plan for these?


--------------------


Important Links.

Be kind to others.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lenz]
    #27054646 -

Lenz said:
Rafiikii said:
any tips with working with swabs?

having trouble with germination.

streaked, removed fragments, dunked the whole swab etc.

still nothing.



Sticking the whole swab into the plate worked for my APE swabs I couldn't get growth from when streaking but if that didn't work for you I think there's a tek floating around here where someone stuck the swab into the agar and then put a couple drops of sterile water on the embedded swab tip.

Might be worth trying, you should be able to find it pretty easily with the search engine.

I haven't worked with swabs enough otherwise to suggest anything else but I'm sure others know more.


its kinda early to call it i guess. maybe some water would help.

never heard of apes germinating slower, is that a thing?


--------------------
"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."


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Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Mix

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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