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Wall.E
Bacteria's Bitch

Registered: 06/05/20
Posts: 3,075
Loc: The Axiom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27022366 - 11/04/20 09:19 PM (5 years, 10 months ago)

Yeah, two longer parallel lines and then you cut through to pull multiple transfers from a row basically. It's a good idea and I'm gonna try it out

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Mehhh

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trippytimes
♡contamination queen♡

Registered: 09/02/14
Posts: 1,220

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27022376 - 11/04/20 09:30 PM (5 years, 10 months ago)

Rafiikii said:

any one try a hexagonal punch?

while looking through my tools, this caught my eye.

think I'm onto something here.

Your stealing my idea! Buy a quartz tube and change the handle..... Heats up good it's clean and heat don't transfer to far with quarts..

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want more info on shrooms "click"

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: trippytimes]
#27022385 - 11/04/20 09:37 PM (5 years, 10 months ago)

Texas Orange Cap

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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TheDoobsker
Amateur

Registered: 05/22/20
Posts: 815

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]
#27022386 - 11/04/20 09:38 PM (5 years, 10 months ago)

mushboy said:

Or! Check out the josex poke/puck method. Highly recommend

Good read that's a pretty slick method. I always trip on how well my myc does in pf jars compared to agar plates. Definitely gonna try this.

So you'd say that Z-strain plate looks bacterial?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,406
Loc: Exact Center
Last seen: 6 hours, 45 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: mushboy]

1
#27022388 - 11/04/20 09:41 PM (5 years, 10 months ago)

mushboy said:

imo punches are a novelty/gimmick at best, unnecessary step/complication at worst

I felt the same way when I first saw it, then I stuck a bit of a stainless steel straw on the end if my scalpel and tried it out, its surprisingly efficient and less movements than cutting wedges ime.

Its like a poke without having to sterilize a whole syringe and squirting water

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: trippytimes]
#27022405 - 11/04/20 09:55 PM (5 years, 10 months ago)

trippytimes said:

Rafiikii said:

any one try a hexagonal punch?

while looking through my tools, this caught my eye.

think I'm onto something here.

Your stealing my idea! Buy a quartz tube and change the handle..... Heats up good it's clean and heat don't transfer to far with quarts..

will try as is for shits and giggles

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

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D3_Myc
Weeb Trash

Registered: 05/06/18
Posts: 4,399
Loc: Year Zero

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27022411 - 11/04/20 10:07 PM (5 years, 10 months ago)

I tried a star shaped one once, too many angles, wedge got mega stuck. I think that the wall thickness will cause your wedge to get compressed into the hole and stick in it. But don’t hurt to fuck around and try.

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D3’s GlogD3's Agar TEKAgar Porn Gallery RW x MelMak Grow Along Thread

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Rafiikii

Registered: 11/17/10
Posts: 2,892

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27022415 - 11/04/20 10:12 PM (5 years, 10 months ago)

D3monic said:

I tried a star shaped one once, too many angles, wedge got mega stuck. I think that the wall thickness will cause your wedge to get compressed into the hole and stick in it. But don’t hurt to fuck around and try.

good point.

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"You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."

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FarmerEric
Friend

Registered: 04/06/20
Posts: 46
Last seen: 5 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27022647 - 11/05/20 05:56 AM (5 years, 10 months ago)

Rafiikii said:

any one try a hexagonal punch?

while looking through my tools, this caught my eye.

think I'm onto something here.

Buy a set of leather punches, wrap the handle in self-sealing silicone tape to block heat.

https://www.amazon.com/OWDEN-12Piece-cutting-leather-material/dp/B078GT892Y/

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,406
Loc: Exact Center
Last seen: 6 hours, 45 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: FarmerEric]
#27022873 - 11/05/20 09:29 AM (5 years, 10 months ago)

Stainless straws are cheaper and don't hold heat long

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

nmd_myco
Obsessed Noob

Registered: 09/05/20
Posts: 198
Loc: PNW
Last seen: 4 years, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Rafiikii]
#27022874 - 11/05/20 09:29 AM (5 years, 10 months ago)

Rafiikii said:

any one try a hexagonal punch?

while looking through my tools, this caught my eye.

think I'm onto something here.

I tried this the day before i found the cocktail straws at target.

The hex tool made a mess and takes 3x as much time to cool down as the straws.

The hex shape did what it would be expected to do... it increased the potential for the tube "grabbing" the sample... a circle provides less points of friction for the puck. think of wiggling one of the hex bits out of those tools... if you torque it too much it gets stuck.

straws are the move. i have a ton of different diameters of thin, fast cooling tube. from 2mm to boba straw width.

Still only use the cocktail straw width because its precut to the perfect length and those silicone tips double up for a perfect handle. grippy, block heat and alcohol-friendly at the same time.

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trippytimes
♡contamination queen♡

Registered: 09/02/14
Posts: 1,220

Re: AGAR ENVY! (Anything and All things agar!)

[Re: nmd_myco]
#27022891 - 11/05/20 09:37 AM (5 years, 10 months ago)

See I use straws but I want to find a quarts rod/straw I figure it would be cleaner and easy to heat and the heat transfer would be less try doing 30 transfer with the same straw burn fingers much

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want more info on shrooms "click"

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: trippytimes]

1
#27022921 - 11/05/20 09:47 AM (5 years, 10 months ago)

Why bother? Just use a scalpel :shrug:

:shrug:

I've done thousands of succesfull transfers without ever once having to worry about that shit.

Extra steps are bad. Less is better.

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,406
Loc: Exact Center
Last seen: 6 hours, 45 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27022943 - 11/05/20 09:55 AM (5 years, 10 months ago)

I'm telling you, its less movements. One for cutting one for picking up as opposed to 3 or 4 to cut and one to lift.

Its not something I'd suggest starting with but if you know what you are doing its pretty nifty

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

nmd_myco
Obsessed Noob

Registered: 09/05/20
Posts: 198
Loc: PNW
Last seen: 4 years, 15 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: D3_Myc]
#27022952 - 11/05/20 10:02 AM (5 years, 10 months ago)

D3monic said:

Texas Orange Cap

beautiful.

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Tight Lunchbox
Drunk cat

Registered: 11/06/16
Posts: 2,137
Last seen: 1 day, 22 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#27022957 - 11/05/20 10:04 AM (5 years, 10 months ago)

How easy is it to transfer the agar to the receiving plate?

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"it's all a joke between mom contractions and coffin fittings"

The most useful tool for noobs

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]
#27022960 - 11/05/20 10:06 AM (5 years, 10 months ago)

tryptkaloids said:

Its not something I'd suggest starting with but if you know what you are doing its pretty nifty

This is pretty much my point. Most people doing this, with a few exceptions, are the sort of people that have trouble producing clean cultures/spawn to begin with. Adding extra steps will not help.

Though there may be fewer hand motions in terms of actual cutting motions, the exposure is greater. 2 tools instead of one, hand exposure by hovering or to manipulate the plate so as not to hover, and increased surface area of transfer.

3 simple cuts is all I make, they are extremely small. Transfer is approximately the size of a pin head.just enough to skewer with my scalpel. 4 tiny/quick motions in total.

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Normlizer420
Stranger

Registered: 02/25/03
Posts: 414

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27023070 - 11/05/20 11:01 AM (5 years, 10 months ago)

I made this using a spent rifle case and old scalpel.

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Inthepit
Aum Mani Padme Hum

Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 1 year, 3 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Stipe-n Cap]
#27023233 - 11/05/20 12:52 PM (5 years, 10 months ago)

Speaking of pin head size transfers.
What about just lifting a piece of myc off the agar.

I did because I fumbled one wedge and then poked the surface
lifting a thread like piece of just myc.

Not sure where that one went, lost track. I recall
it seemed to take awhile to grow.

But it didn't seem to need a wedge under it or over it.

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:sporedrop:

:sporedrop:

:sporedrop:

:sporedrop:

GETTING STARTED :sporedrop:

:sporedrop:

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Stipe-n Cap
Full retard

Registered: 08/04/12
Posts: 9,112
Loc: Canada

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Inthepit]
#27023242 - 11/05/20 12:56 PM (5 years, 10 months ago)

The underlying wedge part of the transfer isn't the important part of the transfer, it's the transportation of clean mycelium that matters.

You'll notice sometimes that your scalpel will have residual mycelium on the blade when you place the wedge on a new plate, if you cut into the receiving plate to deposit the wedge, the cut in the agar will grow a satellite colony independent of the new wedge.

So long as clean mycelium makes it to your receiving plate it doesn't really matter how it gets there. The same thing happens when you transfer a wedge to grain and then give it a shake. The wedge will deposit little fragments of myc on the surface of some of the grains to form satellite colonies independent of the wedge.

Edited by Stipe-n Cap (11/05/20 01:09 PM)

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