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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish]
    #26983313 -

You definitely can, you just don't have a way of guaranteeing the culture you grab is axenic, so you should transfer away from that a couple times to be sure.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish]
    #26983322 -

Certainly.

I am a plate hoarder, and I do this all the time.

Even better is when the overgrown plate pins. Grab a plate pin for a pretty solid clone. Unless you're already working with a clone, it that case, pins are still good, because they will almost always give you clean growth. At least in my experience

But also :whathesaid: still may need a couple transfers before its all clean


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LAGM 2021
LAGM 2020
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Re: AGAR ENVY! (Anything and All things agar!) [Re: One of Us]
    #26983434 -

I use overgrown plates all the time... its up to you how much you want to risk...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: One of Us]
    #26983685 -

Using a plate pin sounds awesome lol. Do you use tweezers instead of a scalpel or is there some other strat for grabbing them?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: LoadedFish]
    #26983713 -

They come up pretty easily with either scalpel or tweezers.
I've tried to get into using tweezers for different things, but I always end up just learning a knife technique that works seemingly better than any tweezing.

edit: the technique for plate pins is harpoon.

Edited by footpath (10/13/20 07:09 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #26984235 -

So i got a bit careless with my agar and the last batches have all had bacterial problems.

I switched it up and used some bigger pp5 dishes didnt poke any GE holes (i read somewhere on hear that it wasnt necesary as there is enough gas in the bigger container)...
But all of these agars have got growth that is not very white... its growth (it seems) but strange. Iv seen from searching sometimes it can be nutrient deficiency but mine dont seem to look the same and there is LME + drops of honey. I do no pour tek.

Im hoping someone might have a hint for me... first time posting pictures and its harrrrd with that glare. Apologies and many thanks to anyone who can shed some light for me.

Iv gone back to using distilled water for agar and poking holes. Basic recipe. The recipe in these pics has coffee and food coloring.



Edit: i should prolly mention i dropped two transfers in each here (hence two growths) and they are both pictures of rustywhytes transfers about 7 days ago 🤙

Edited by Galendula (10/14/20 12:31 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Galendula]
    #26984240 -

Maybe try some gentamicin sulfate powder or Chloramphenicol.  I am not sure if the community consensus has changed on these in agar, but I have read a in few books that they can be added to help with bacteria.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Galendula]
    #26984273 -

How come youre using honey and all that? It seems just agar and lme is sufficient.. Might be easier to narrow down where your issue is coming from with less ingredients. If you wanna stick with it id see if youve changed anything? New honey brand perhaps?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Robin-Forepaper]
    #26984286 -

I like to use 8g Agar, 7g LME for germinating spores and 9g Agar, 8g LME for transfers.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fightingcherries]
    #26984302 -

☝️☝️☝️ iv seen some opposite findings on using those antibacs but ill give it another check. Thx ::)

Honestly my original agar i could only find the liquid barley malt extract and it actually worked well...

I was always getting tomatose growth and so i went out and sourced the brewers lme... found some recipes and some used honey so started doing that and adding a drop to each 250ml batch. Its not real honey tbh i think its more like sugar syrup and iv used the same bottle with success.

After that i started adding the food coloring and then progressed to the coffee which was a youtube vid recipe (im growing a few varieties of oysters too)

I got a bit excited and i think ill go back to absolute basics with your recipe above (thank you all replies)

Do you think these transfers are screwed? Im a big fan of agar.. ill most definatly let them continue to see what happens but im wondering if its worth taking a transfer from them... or if its not enough GE. Im curious why it looks that way.

Ahhh my science side has not caught up to my wonder side... one day!
Im still pretty new and i get excited to try new things. I also was pretty lucly at failing hard early on and then building my Sab which changed everything... i guess i got too ambitious ^^

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Galendula]
    #26984362 -

If youre only gettin tomentose perhaps you should try less nutes? Even if you use the same ingredients you could try cutting the nutrient portion back. I dont think they're toast.. Depends how bad you want to save them and how important they are, imo it's probably not worth the effort if you got others on the go. If you do choose to try and save it? Id hit about 10 and 4-5 o clock ish. On pic 1 top culture. Good luck my guy!

Edited by Robin-Forepaper (10/14/20 02:36 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Robin-Forepaper]
    #26984513 -

RW is typically tomentos. I’ve only gotten rhyzo on a few plates and it was after T7 or so. I just likes to stay fluffy.



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26984538 -

I somehow got the most my rhizo growth so far with Rw but it was on bacteria ridden plates! Iv tried to transfer out using h2o2 so if it works ill upload pics..

Happy to hear though coz i also just dropped a dish of tomo Rw to grain because im all about 'why not!?' right now 😆

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Galendula]
    #26984554 -

Personally, I would toss them and start fresh but I don't think they're a complete loss. You can do a hot pour and take transfers when the myc comes back to the surface and grows away away from the bacteria.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Galendula]
    #26984562 -

Galendula said:
So i got a bit careless with my agar and the last batches have all had bacterial problems.

I switched it up and used some bigger pp5 dishes didnt poke any GE holes (i read somewhere on hear that it wasnt necesary as there is enough gas in the bigger container)...
But all of these agars have got growth that is not very white... its growth (it seems) but strange. Iv seen from searching sometimes it can be nutrient deficiency but mine dont seem to look the same and there is LME + drops of honey. I do no pour tek.

Im hoping someone might have a hint for me... first time posting pictures and its harrrrd with that glare. Apologies and many thanks to anyone who can shed some light for me.

Iv gone back to using distilled water for agar and poking holes. Basic recipe. The recipe in these pics has coffee and food coloring.



Edit: i should prolly mention i dropped two transfers in each here (hence two growths) and they are both pictures of rustywhytes transfers about 7 days ago 🤙


Honey, coffee... let’s start over with the basics and see how it does. I wouldn’t even waste my time trying to rescue myc out of those samples. More effort than starting over.

Pp5 is permeable and allows gas exchange. Though if you do decide to have ventilation you are covering the hole with micropore tape correct?

Let’s start with a super basic agar recipe that works extremely well. 10g agar, 10g lme and 500ml distilled water. This should work well for germination of spores , provide plenty of nutrients ect ect. You can play with the nute levels on subsequent transfers. I personally run 20g agar, 13g lme and 1000ml distilled which gives me 40 100mm petris


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26985571 -

Texas Orange Cap



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26985595 -

D3monic said:
Texas Orange Cap




Damn, bro! I wish I could get the picture quality that you put out.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26985604 -

D3monic said:
Texas Orange Cap




kinda cliche at this point to call one of your plates sexy.

As for the photo quality... i am going to get a fixed mini tripod and mark a special spot in the hood with a fixed distance and a camera or camera app that will fix my focus easily. then its just a question of beating the glare. the problem for most is that the software focuses on the lid of the plate because it reflects light. you can focus through it, but hard to do without a VERY steady hand, which i dont have.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nmd_myco] * 1
    #26985631 -

Shooting plates definitely takes some getting used to. It’s like whack a mole. I’ll get it to focus right but then there’s glare, I get the glare out but then you can see my face on the lid.


I hear people talk about girls and selfie angles but I think we might have them beat.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nmd_myco] * 1
    #26985637 -



So... been waiting till I had something ON agar to post any of my work on this thread.  Today, I prepped for T1's on Friday.  I took a couple practice transfers over the last couople of days, but I am waiting for some myc that leaps out at me.

Lessons learned about putting minimal spores to agar. I am going to fuck with serial dilutions next time I get something special.

The nicest colonies I'm seeing are from a recently made TC's PESA and Costa Rica- Arenal. The vigor out of the gate that I am seeing on the Arenal and comparing it to the proven PESA, I'm excited that this is the one thats really going for it out of my rando spores from randomspores.com.

I have a PE from another user here that I am going to get onto plates soon.  I was sorta just waiting to prove to myself that I can do this before I try swabs.  Seems easier to fuckup and waste 2 swabs than to ruin a whole print or syringe.



Thanks to everybody for all the knowledge.

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Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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