agar said:

If you have STERILE SWABS ?

Simply drop a drop of the culture solution on the swab, then streak the swab LIGHTLY across the agar plate.

This works BETTER, if you have an LC that was shredded by a stir plate stir bar. As the myc is chopped into minuscule pieces.


If you don't have sterile swabs, simply push the syringe plunger down, a teeny weeny bit, until a tiny drop of LC forms on the needle.

Then use the needle to drag the drop across the face of the plate.

If all else fails, drop a drop of LC on the center of the plate, then tilt the plate & try to roll the droplet across the face of it.

As I understand it, you injected sterile water into a (partially filled) colonized wbs/grain jar.

I would suggest you quickly use sterile syringes to extract all the solution possible. Then refrigerate those syringes.

All that water, in all that grain - is going to cause gooey problems, if left alone.

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Thanks, man. 

I know any colonized jar injected with water is to be forgotten. 

I'll give it a go.  I'm going to wait until there's very thick colonization, then inject, say, 200--300ml, shake it about in the glovebox, then pull out a few LC culture syringes from that.  Ditch the jar afterwards.  I'll try to spawn the other fully colonized jars to pasteurized wood substrate.

Also: I FUCKING MANAGED to clone a Dryad's Saddle into LC from the stump out back.  I'm a bit excited about this--first time. 

But that shit is colonizing the spawnbag fine (one out of like 6 jars of LC dryad saddle attempts).  So I know which jar is active.  I'll play around with that one too. 

I'd just like to get some experience with agar cultures.

Thanks, tho.  You're a fucking bastion mycoknowledge.  Hats off, sir. 


Also...that pic sort of looks like a butt.    That's the OTD coming out in me now, tho.