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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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PURE WHITE LIQUID CULTURE PANCAKES
    #3978875 -



Have many bulk liquid cultures going.

I forgot to stir a few for awhile (week - 10 days?) :confused: on the stir plate.

Ones I didn't stir, grew about an 1/8 or 3/16ths thick pure white myc pancake floating on the top.

Have on stir plate now, trying to break it up & get everything back into fluid suspension.

LOL...... myc pancake is pretty tough - to get apart :eek:.


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3978888 -

you got any pics of your stir plate or inf on where you got it? ive been looking for a good method of mixing up my LCs

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: bixo]
    #3978913 -


Watch eBay. Got this powerfull old workhorse for $30 + S/H
(Flash freezes stir bar.)


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3978920 -

Ahh nice man.. so you just got something that you leave in the liquid culture and the magnetic stir machine gets that object spinning?

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: bixo]
    #3978950 -

Stir bar goes in the jar - pre/PC.

if you get one, PM me & I will show you how to build stir bars, for almost nothing. Store bought, they run about $9 + each. Which can get spendy, running a dozen or 2 culture jars - at once.

EBAY EXAMPLE LINK


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3978962 -

agar said:

Watch eBay. Got this powerfull old workhorse for $30 + S/H
(Flash freezes stir bar.)


j00 R h4Dk0r3 4 lyfe.

:bow: :laugh:


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Jerry Garcia. JERRY GARCIA! JERRY GARCIA!!!!

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: GnuBobo]
    #3978992 -

:thumbup:

Who's hungry Who's hungry (oldschool) :rotfl:

-Gnostic


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For the sake of those seeking knowledge.
I've provided some in my signature.
Double Tek Pictorial
Getting the most out of your casings!, A pinning strategy.
Soaking wbs - The Final Answer by Agar
50/50 Coco Coir versus 50/50 Peat Moss.

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3979264 -

I like my liquid culture jars with lots of injection plugs :grin:


one in action (almost gone)


Throw me a PM about those cheap stir bars if you don't mind Agar :thumbup:

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: MrMaddHatter]
    #3979294 -


>>Throw me a PM about those cheap stir bars if you don't mind<<

Done did.

Hell, I just should have posted it. Copy & post it - would you. I didnt save it.


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3979302 -

Go to auto parts store. Buy several feet rubber wind sheild wiper type vaccum hose. Also buy RTV high temp gasket sealer.

Find steel nail, bolts or threaded rod(magnetic) that will fit tight in rubber hose (Ace or True Value). Cut hose into into short lengths, cut of heads of bolts/nails - or cut rod to size, insert in hose - seal both ends with RTV sealant. Allow to cure overnight.

BAM :grin:

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: MrMaddHatter]
    #3979310 -

Thank you Sir.  :wink:


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #3979318 -

thanks for the info agar..time to start some ebay huntin..

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: bixo]
    #3980449 -

Good idea on the stir bar.

I had a myc pancake form in a culture I think. I didn't stir for 3-4 days though. I didn't think the myc would form a solid that fast so I tossed it thinking it may be a contam. It was pure white and looked just like your pics but only took up 25% of the surface.

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: Smushroom]
    #3980491 -

Surface myc starts small & grows. If it was pure white. Sounds like you tossed a good jar.


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #4243048 -

question on those stir bars, can that windshield tubing withstand autoclave temperatures. Say for making those nifty lids you have?


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Check out the official MushroomWiki at http://www.mushroomwiki.com. Stop by and contribute.

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: xburn]
    #4243059 -

agar- do you believe harvesting the mycelium is worthwhile? I have been toying with the idea of a 50l container. I already have it all built for another purpose and it could be easily modified for a bulk LC.
An idea that I tried before was readding sugars to a spent LC, theory being that after it uses up the 4% solution it is thick with mycelium, you bring it to 4% again and it will multiply again very quickly ending up in a densely populated mycelium LC. It seemed to work for a while, I never drank it though. After a while its metabolites should become toxic to itself.

Edited by blackout (06/01/05 03:27 AM)

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: xburn]
    #4243064 -

xburn said:
question on those stir bars, can that windshield tubing withstand autoclave temperatures. Say for making those nifty lids you have?


Most plastic is fine in a a/c. You could always just cover the nail completely with silicon

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: blackout]
    #4243151 -

hey agar, thats basically THE example of overlay :wink:
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: blackout]
    #4243425 -

blackout said:
agar- do you believe harvesting the mycelium is worthwhile?


Years ago, I did a 5 gallon batch that was stirred & provided with filtered air exchange. The results were not worth the time, effort or trouble. Moreover, LC's are very prone to contamination. In the sense that a liquid nutriant broth is an ideal medium for them.

The big LC culture was incubated in darkness. I have always wondered - if once the culture was fairly thick - if it was exposed to steady light & incubated/stirred for more time (a week)- if that might increase potency?

All in all.... I believe fruiting out an optimal substrate under ideal conditions will get you about the best potency - possible.


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Re: PURE WHITE LIQUID CULTURE PANCAKES [Re: agar]
    #4243474 -

Speakin of potency increases in LC's. I've been fiddling around with vanilla in LC's. Vanillas loaded with amino acids and anti's. I used some extract in one to see if it would be worth while. Just to get a colonization and MAYBE a potency increase when knockin up grains. You know all that jazz about Tryptophan increasing potency? And to my surprise the damn LC is taking off like a rocket. Haven't put it to use yet though.

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Shop: Mushroom-Hut Substrate Mix   MagicBag.co Certified Organic All-In-One Grow Bags   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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