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Offlinespliffmasta
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Can u clone liquid cultures?
    #3364588 - 11/15/04 04:57 PM (13 years, 29 days ago)

...assuming that that the liquid culture was less than 2 weeks old, or do you need to use tissue, spores, or a solid substrate or somethin?


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Offline4hodmt
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364602 - 11/15/04 05:03 PM (13 years, 29 days ago)

yes.


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Offlinerecalcitrant
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364616 - 11/15/04 05:05 PM (13 years, 29 days ago)

mycelium grows. Suck up some liquid culture and squirt it into a new jar of karo. I can't see why this wouldn't work.

I hear say of potency loss, but I can't understand it with taking some mycelium and giving a new place to grow. :confused:


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: 4hodmt]
    #3364617 - 11/15/04 05:06 PM (13 years, 29 days ago)

Hrmm, can you think of a better method to grow mushrooms then than using a perpetuated supply of liquid culture and spawning it to bulk? You don't even need a PC!


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364629 - 11/15/04 05:08 PM (13 years, 29 days ago)

Sure. You can take a sample from 1 karo jar and transfer it to another. It will only be a clone if the 1st karo jar was started with cloned material, though.

As I understand it, it has to do with age. Cells are only designed to replicate themselves so many times before, during the replication process, errors occour and you have genetic degredation.


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Offlinerecalcitrant
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Re: Can u clone liquid cultures? [Re: discman1]
    #3364650 - 11/15/04 05:13 PM (13 years, 29 days ago)

Ahh, right. Much appreciated.


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364655 - 11/15/04 05:16 PM (13 years, 29 days ago)

Got any clue as to how many generations it takes before that happens?

Also, I have cheap Meijer brand honey, but it says "Pure Clover Honey US Grade A" and on the back there are no indications of additives or additional ingredients at all. Would this work for a liquid culture?


Edited by spliffmasta (11/15/04 05:18 PM)


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Offlinerecalcitrant
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364674 - 11/15/04 05:20 PM (13 years, 29 days ago)

I don't know how long it takes for a fungal cell to replicate itself, but I heard on the radio or something that a cell divides fifty times before it dies.

The program went on to explain that there is research into extending that number indefinately. Aparently in the early part of genetic research someone thought they found the secret, and it pissed off every scientist who tried to copy his experiement because they couldn't get their cultures to survive. I guess the original experiment was completely bunk because it wasn't immortal cells, just regular reproduction, but no one figured that out for fifty years.

Surely that jumble of layspeak clears everything up :wink:


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364693 - 11/15/04 05:24 PM (13 years, 29 days ago)

What about this honey though, cause I'm gonna do these suckers this instant if this is the stuff :wink:


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364714 - 11/15/04 05:28 PM (13 years, 29 days ago)

If it says it's pure honey, it shouldn't have anything else in it.

Why do you want to use honey, though? I was under the impression that it is less desirable than karo, because honey is colored and contains proteins and other substances that can further cloud the mixture, making it more difficult to discern whether you've got mycelium or contamination.


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: discman1]
    #3364746 - 11/15/04 05:35 PM (13 years, 29 days ago)

Cause I'm sittin here bored, reading, with nothing to do, waiting for my PC to be delivered and I have these ingredients on my desk with me.

What is that stuff anyway, and where do I get it?


Edited by spliffmasta (11/15/04 05:35 PM)


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364755 - 11/15/04 05:37 PM (13 years, 29 days ago)

Karo is just a brand name of light corn syrup.

You can pick up a bottle of generic light corn syrup for less than 2$ at any store that carries baking supplies.



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Offlinerecalcitrant
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Re: Can u clone liquid cultures? [Re: discman1]
    #3364784 - 11/15/04 05:42 PM (13 years, 29 days ago)

holy shit, those are big clusters discman. Do you shake your karo?


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364803 - 11/15/04 05:45 PM (13 years, 29 days ago)

That was after 2 weeks. I've shaken it a few times, but it doesen't do anything. They just swirl around in there... lol.

I wasn't under the impression that it matters? I haven't used that jar yet, but when I do.. I will just use a syringe and sucking/spitting action to break the balls up before the final draw.


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Offlinerecalcitrant
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Re: Can u clone liquid cultures? [Re: discman1]
    #3364810 - 11/15/04 05:47 PM (13 years, 29 days ago)

I tried making some karo just recently. I've been stirring it everyday and I see many tiny clusters forming all over. Nothing like that


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364820 - 11/15/04 05:49 PM (13 years, 29 days ago)

How long does the colonization take and how big of a batch is it safe to make?


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364826 - 11/15/04 05:50 PM (13 years, 29 days ago)

Yeah. I have two karo jars of that strain. The 1st jar is pictured, the 2nd jar is quite a bit different.. It has many more thinner, smaller.. more wispy blobs. Swirling that one around does indeed break them up a bit.


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Offlinediscman1
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Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364838 - 11/15/04 05:52 PM (13 years, 29 days ago)

Quote:

spliffmasta said:
How long does the colonization take and how big of a batch is it safe to make?


I believe you should give at least 1.5 weeks before taking a sample. You want to make sure there is plenty left in there to re-colonize.

You can make them as big as you want, as long as they will fit into your PC, really... but there's no reason to go nuts and make a quart jar of karo or anything wacky, lol. It'd be better to make many smaller samples, so you don't lose all if one gets contaminated.


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OfflineMushroomFriend
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Re: Can u clone liquid cultures? [Re: discman1]
    #3364849 - 11/15/04 05:55 PM (13 years, 29 days ago)

Discman do you know if any kind of grain syrup will work? Cause here I dont know karo or mais syrup.


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Offlinespliffmasta
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Re: Can u clone liquid cultures? [Re: MushroomFriend]
    #3364881 - 11/15/04 06:04 PM (13 years, 29 days ago)

Do you have to use a pressure cooker, will steam sterilizing not work?


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