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OfflineInjectTruth
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Cloning in liquid culture, is it possible?
    #3131003 - 09/13/04 08:36 PM (13 years, 7 months ago)

hello. Ive recently been reading up on cloning with cardboard and also making liquid cultures. Can you put a piece of mush flesh directly in a sterile karo solution to clone it? or should you clone with cardboard and use a piece of that? Seems like you could do it.


--------------------
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Offlinehyphae
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Re: Cloning in liquid culture, is it possible? [Re: InjectTruth]
    #3131280 - 09/13/04 09:40 PM (13 years, 7 months ago)

I have done this with just sterile water no need for Karo unless your looking at some kinda long time storage. I just take a very nice chunk (1-2") as long as possible and dropped it into my homemade Eberbach which was PCed with h2o in it. It makes for some nice quick cloning. I also use CBS's (core biopsy syringes) after Mycota (6T) turned me on to them a few years back. They make taking sterile samples a breeze!!!!!


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Invisiblefastfred
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Re: Cloning in liquid culture, is it possible? [Re: hyphae]
    #3132202 - 09/14/04 12:24 AM (13 years, 7 months ago)

That may be fine for people with a fancy blender, but you can also just drop a small piece in some 3% malt extract solution and let it grow up.


-FF


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OfflineInjectTruth
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Re: Cloning in liquid culture, is it possible? [Re: fastfred]
    #3133641 - 09/14/04 11:25 AM (13 years, 7 months ago)

great. that makes life easier. Hyphae, whats a core biopsy syringe? I'll see if I can do a search.


--------------------
On a personal level, Freaking Out is a process whereby an
individual casts off outmoded and restricting standars of
thinking, dress, and social etiquette in order to express
CREATIVELY his relationship to his immediate environment and
the social structure as a whole.

http://www.OrganicPharming.com - Ethno Shopping Portal


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Offlinehyphae
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Re: Cloning in liquid culture, is it possible? [Re: InjectTruth]
    #3134084 - 09/14/04 01:33 PM (13 years, 7 months ago)

CBS's are used for taking sterile tissue samples from organs, tumors, mushrooms, etc; They are also reusable. :laugh:





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OfflineInjectTruth
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Registered: 10/05/03
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Re: Cloning in liquid culture, is it possible? [Re: hyphae]
    #3134220 - 09/14/04 02:15 PM (13 years, 7 months ago)

hmm.  how bout dat? :animal:


--------------------
On a personal level, Freaking Out is a process whereby an
individual casts off outmoded and restricting standars of
thinking, dress, and social etiquette in order to express
CREATIVELY his relationship to his immediate environment and
the social structure as a whole.

http://www.OrganicPharming.com - Ethno Shopping Portal


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Invisiblefastfred
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Re: Cloning in liquid culture, is it possible? [Re: InjectTruth]
    #3134264 - 09/14/04 02:29 PM (13 years, 7 months ago)

Do you have a source for those biopsy syringes hyphae?

-FF


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Offlinehyphae
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Re: Cloning in liquid culture, is it possible? [Re: fastfred]
    #3135561 - 09/14/04 07:33 PM (13 years, 7 months ago)

When available I've seen heard them go from $35 on up to $75 each. I do have a source but it's inconsistant so basically I put my order in and wait, last time it was almost 3mos. :wink: When I get them they go for $35+shipping (2-3 day delivery). These shoot the needle out and back into a sheath keeping the sample from ever being exposed to an open environment, I like to store the syringe in a bottle of alcohol right up until the sample is taken. :wink:


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OfflineGr0wer
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Re: Cloning in liquid culture, is it possible? [Re: hyphae]
    #3135855 - 09/14/04 08:44 PM (13 years, 7 months ago)

Could we see the homemade Eberbach? Possibly on a new thread with pointers and info? It could even be added to the tek's :wink:


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Offlinehyphae
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Re: Cloning in liquid culture, is it possible? [Re: Gr0wer]
    #3136048 - 09/14/04 09:31 PM (13 years, 7 months ago)

Just looked and it must be in storage, when I get a chance I'll grab it and take a pic for ya guys :wink: It's really quite simple, it is a regular mouth pint jar screwed to a blender base (poly carbonate), not all bases work, my first deformed badly (Proctor Silex) but the second one (Oster) worked flawlessly. The base was drilled and tapped with a small machine screw which is where the syringe is inserted after the screw is removed. It works great when used with the CBS (core biopsy syringe!) :wink: take care guys


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