Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   MagicBag.co Certified Organic All-In-One Grow Bags   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Can u clone liquid cultures?
    #3364588 - 11/15/04 02:57 PM (19 years, 4 months ago)

...assuming that that the liquid culture was less than 2 weeks old, or do you need to use tissue, spores, or a solid substrate or somethin?

Extras: Filter Print Post Top
Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 9 years, 10 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364602 - 11/15/04 03:03 PM (19 years, 4 months ago)

yes.


--------------------
all posts made by 4hodmt are entirely ficticious. the user 4hodmt has noa ffiliation with any company, or organization; any endorsements made by 4hodmt are to be considered a joke. any similarity between a person (or story) 4hodmt is talking about is entirely happenstance. 4hodmt is not to be taken seriously under any circumstance. furthermore, he does not know what he is talking about. Please ignore anything 4hodmt has to say.


_______

"Trust me, I'm from the future.


Actually I'm not from the future, but I did lived there for a while."

- Baby Hitler

Extras: Filter Print Post Top
Offlinerecalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 7 years, 10 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364616 - 11/15/04 03:05 PM (19 years, 4 months ago)

mycelium grows. Suck up some liquid culture and squirt it into a new jar of karo. I can't see why this wouldn't work.

I hear say of potency loss, but I can't understand it with taking some mycelium and giving a new place to grow. :confused:


--------------------

We have to answer our own prayers

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: 4hodmt]
    #3364617 - 11/15/04 03:06 PM (19 years, 4 months ago)

Hrmm, can you think of a better method to grow mushrooms then than using a perpetuated supply of liquid culture and spawning it to bulk? You don't even need a PC!

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364629 - 11/15/04 03:08 PM (19 years, 4 months ago)

Sure. You can take a sample from 1 karo jar and transfer it to another. It will only be a clone if the 1st karo jar was started with cloned material, though.

As I understand it, it has to do with age. Cells are only designed to replicate themselves so many times before, during the replication process, errors occour and you have genetic degredation.

Extras: Filter Print Post Top
Offlinerecalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 7 years, 10 months
Re: Can u clone liquid cultures? [Re: discman1]
    #3364650 - 11/15/04 03:13 PM (19 years, 4 months ago)

Ahh, right. Much appreciated.


--------------------

We have to answer our own prayers

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364655 - 11/15/04 03:16 PM (19 years, 4 months ago)

Got any clue as to how many generations it takes before that happens?

Also, I have cheap Meijer brand honey, but it says "Pure Clover Honey US Grade A" and on the back there are no indications of additives or additional ingredients at all. Would this work for a liquid culture?

Edited by spliffmasta (11/15/04 03:18 PM)

Extras: Filter Print Post Top
Offlinerecalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 7 years, 10 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364674 - 11/15/04 03:20 PM (19 years, 4 months ago)

I don't know how long it takes for a fungal cell to replicate itself, but I heard on the radio or something that a cell divides fifty times before it dies.

The program went on to explain that there is research into extending that number indefinately. Aparently in the early part of genetic research someone thought they found the secret, and it pissed off every scientist who tried to copy his experiement because they couldn't get their cultures to survive. I guess the original experiment was completely bunk because it wasn't immortal cells, just regular reproduction, but no one figured that out for fifty years.

Surely that jumble of layspeak clears everything up :wink:


--------------------

We have to answer our own prayers

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364693 - 11/15/04 03:24 PM (19 years, 4 months ago)

What about this honey though, cause I'm gonna do these suckers this instant if this is the stuff :wink:

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364714 - 11/15/04 03:28 PM (19 years, 4 months ago)

If it says it's pure honey, it shouldn't have anything else in it.

Why do you want to use honey, though? I was under the impression that it is less desirable than karo, because honey is colored and contains proteins and other substances that can further cloud the mixture, making it more difficult to discern whether you've got mycelium or contamination.

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: discman1]
    #3364746 - 11/15/04 03:35 PM (19 years, 4 months ago)

Cause I'm sittin here bored, reading, with nothing to do, waiting for my PC to be delivered and I have these ingredients on my desk with me.

What is that stuff anyway, and where do I get it?

Edited by spliffmasta (11/15/04 03:35 PM)

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364755 - 11/15/04 03:37 PM (19 years, 4 months ago)

Karo is just a brand name of light corn syrup.

You can pick up a bottle of generic light corn syrup for less than 2$ at any store that carries baking supplies.


Extras: Filter Print Post Top
Offlinerecalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 7 years, 10 months
Re: Can u clone liquid cultures? [Re: discman1]
    #3364784 - 11/15/04 03:42 PM (19 years, 4 months ago)

holy shit, those are big clusters discman. Do you shake your karo?


--------------------

We have to answer our own prayers

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364803 - 11/15/04 03:45 PM (19 years, 4 months ago)

That was after 2 weeks. I've shaken it a few times, but it doesen't do anything. They just swirl around in there... lol.

I wasn't under the impression that it matters? I haven't used that jar yet, but when I do.. I will just use a syringe and sucking/spitting action to break the balls up before the final draw.

Extras: Filter Print Post Top
Offlinerecalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 7 years, 10 months
Re: Can u clone liquid cultures? [Re: discman1]
    #3364810 - 11/15/04 03:47 PM (19 years, 4 months ago)

I tried making some karo just recently. I've been stirring it everyday and I see many tiny clusters forming all over. Nothing like that


--------------------

We have to answer our own prayers

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364820 - 11/15/04 03:49 PM (19 years, 4 months ago)

How long does the colonization take and how big of a batch is it safe to make?

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: recalcitrant]
    #3364826 - 11/15/04 03:50 PM (19 years, 4 months ago)

Yeah. I have two karo jars of that strain. The 1st jar is pictured, the 2nd jar is quite a bit different.. It has many more thinner, smaller.. more wispy blobs. Swirling that one around does indeed break them up a bit.

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364838 - 11/15/04 03:52 PM (19 years, 4 months ago)

Quote:

spliffmasta said:
How long does the colonization take and how big of a batch is it safe to make?


I believe you should give at least 1.5 weeks before taking a sample. You want to make sure there is plenty left in there to re-colonize.

You can make them as big as you want, as long as they will fit into your PC, really... but there's no reason to go nuts and make a quart jar of karo or anything wacky, lol. It'd be better to make many smaller samples, so you don't lose all if one gets contaminated.

Extras: Filter Print Post Top
OfflineMushroomFriend
I smell a conspiracy!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 21 days, 20 hours
Re: Can u clone liquid cultures? [Re: discman1]
    #3364849 - 11/15/04 03:55 PM (19 years, 4 months ago)

Discman do you know if any kind of grain syrup will work? Cause here I dont know karo or mais syrup.


--------------------

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: MushroomFriend]
    #3364881 - 11/15/04 04:04 PM (19 years, 4 months ago)

Do you have to use a pressure cooker, will steam sterilizing not work?

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3364906 - 11/15/04 04:11 PM (19 years, 4 months ago)

What other kind of grain syrups are there? Not really sure.

I'd say when in doubt use honey. Actually, I asked the question a few days ago and didn't really get much of a response, but I'm wondering why you can't just use water with table sugar dissolved in it.

You should be able to use steam sterilization for liquid culture, yeah.

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: discman1]
    #3364934 - 11/15/04 04:19 PM (19 years, 4 months ago)

Does long grain brown rice have to be PC'd if it's not ground up?

Edited by spliffmasta (11/15/04 04:19 PM)

Extras: Filter Print Post Top
OfflineMushroomFriend
I smell a conspiracy!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 21 days, 20 hours
Re: Can u clone liquid cultures? [Re: discman1]
    #3365047 - 11/15/04 04:50 PM (19 years, 4 months ago)

I read about rice- and wheatsyrup.

Ill be looking for corn syrup first though or some straight honey..

Sorry but I havent got a clue about that sugar in water question, but you probably know that... :smile:

Thanks!


--------------------

Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Can u clone liquid cultures? [Re: MushroomFriend]
    #3365091 - 11/15/04 05:00 PM (19 years, 4 months ago)

I use 1 cc of (or was it 2cc) molasses in water (frutopia bottle)then I sterilize it.
details here along with my strange way of making liquid innoculant
http://members15.clubphoto.com/peter627123/2058710/guest.phtml
the rotator thing keeps everything suspended nicely and the agar in the slant partially dissolves making a food soup that coats the grains.creepy fast colonization.I'm working on a better write up this week to post but you can get the general idea.

Peterthinks


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
OfflineMushroomFriend
I smell a conspiracy!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 21 days, 20 hours
Re: Can u clone liquid cultures? [Re: Peterthinks]
    #3365261 - 11/15/04 05:24 PM (19 years, 4 months ago)

Cool you professor! :laugh:


--------------------

Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Can u clone liquid cultures? [Re: MushroomFriend]
    #3365568 - 11/15/04 06:07 PM (19 years, 4 months ago)

Heh Heh!
You may want to use the ground wire (Green)if you build one!
I just looked at the picture closer and I notice that I didn't use mine!
ZOT! I should be more carefull.
I once took 220 volts from nipples to knees and lived(barely!)
that's twice house voltage.
110 just doesn't scare me that much anymore.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Can u clone liquid cultures? [Re: Peterthinks]
    #3365598 - 11/15/04 06:11 PM (19 years, 4 months ago)

Oh,if you don't have a 40 rpm gear motor kicking around a larger clock's second hand would probably work with a carefully placed dab of crazy glue to keep the hand from slipping.I got the motor I used surplus for $2 at princess auto.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: Peterthinks]
    #3366159 - 11/15/04 07:59 PM (19 years, 4 months ago)

Yo disc, I just noc'd up some regular table sugar, I'll let you know how it comes out.

Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3366236 - 11/15/04 08:16 PM (19 years, 4 months ago)

That would be fantastadilastic.
how much water what type(tap distilled boiled fishtank)
and how much sugar?.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: Peterthinks]
    #3366253 - 11/15/04 08:21 PM (19 years, 4 months ago)

I boiled my water for 30 minutes. I put about 200 mL in a gladware with 2 holes in the lid filtered with polyfil, then I added a seemingly arbitrary amount of sugar that was probably about 3 x table spoons. Then I added a wee dash of H2O2, then microwaved on high for 30 minutes. Then noc'd it up with about 3 cc through the filter. Got it incubatin..

Extras: Filter Print Post Top
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3366306 - 11/15/04 08:36 PM (19 years, 4 months ago)

Well too much can be bad for growth.sugar is a preservative too.
but I have agar in my tubes for food too so who knows....a weaker solution may work for me because of that.
If it doesn't take try a weaker solution or just add sterile water.
or try both and see which works better.


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.

Extras: Filter Print Post Top
Offlinediscman1
journeyman
Registered: 08/24/04
Posts: 962
Last seen: 19 years, 3 months
Re: Can u clone liquid cultures? [Re: Peterthinks]
    #3366472 - 11/15/04 09:15 PM (19 years, 4 months ago)

For karo, 1tsp - 1tbsp per 100ml of water works well. So I'd say there is a pretty wide margin.

Extras: Filter Print Post Top
Offlinespliffmasta
Fuzzy thumb
Registered: 10/20/04
Posts: 764
Loc: Casing layer of hell
Last seen: 19 years, 11 days
Re: Can u clone liquid cultures? [Re: discman1]
    #3366773 - 11/15/04 10:09 PM (19 years, 4 months ago)

Yea I operate on about 1 tbsp/100 mL

Extras: Filter Print Post Top
Offlinefungophiliac
militantpacifist
 User Gallery
Registered: 11/08/04
Posts: 190
Loc: yo mama
Last seen: 18 years, 6 months
Re: Can u clone liquid cultures? [Re: spliffmasta]
    #3459526 - 12/07/04 12:19 PM (19 years, 3 months ago)

i thought agar was just a medium for surface grawth i didnt know it was nutritive to mycelium.


--------------------
why are there so many more horses asses than there are horses?

if you can't duck it, fuck it.
-the makers of duck tape


Extras: Filter Print Post Top
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 2 months
Trusted Cultivator
Re: Can u clone liquid cultures? [Re: fungophiliac]
    #3461725 - 12/07/04 08:37 PM (19 years, 3 months ago)

Agar agar is a medium for surface growth and isnt nutrious. Thats why you add a nute when making "agar". Agar is a nute (malt or potatoe dextrose or sugar or whatever), water, and agar agar.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   MagicBag.co Certified Organic All-In-One Grow Bags   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 53,106 43 07/08/23 07:04 AM
by Screwup
* GUNK IN LIQUID CULTURE
( 1 2 all )
agar 6,037 20 03/29/05 05:24 AM
by Sbealzebub
* Dextrose liquid culture Flashman 9,211 12 01/16/05 06:08 PM
by Psilocybeingzz
* Malt extract - liquid culture _JJ_ 1,731 1 04/21/05 10:41 AM
by Anno
* Cloning in liquid culture, is it possible? InjectTruth 2,031 9 09/14/04 07:31 PM
by hyphae
* Liquid culture problems simplemachine 3,289 10 11/12/03 08:17 PM
by deanofmean
* dumb liquid culture cloning question Anonymous 2,236 9 11/17/04 10:29 AM
by derx
* Help! Liquid culture on grain going bad wrong Submob 2,561 4 10/26/03 03:01 AM
by Submob

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,932 topic views. 30 members, 124 guests and 98 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.009 seconds on 14 queries.