Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Liquid culture problems
    #2096563 -

I've been working with liquid culture following this recipe...

50cc of water and 1/2 teaspoon karo syrup in a jar. This is mixed up sterilized for 10 min in boiling water, and innoculated.

When incubated at apx 85f I have germination and mycellial threads within 3-4 days which congeal into floating clumps over about 2 weeks.

My problem is that the jars just don't seem to fill up like I'd expect them to. I have heard that a liquid culture should be used when the mycellium fills half the jar. This is WAY under what I get. I have tried both distilled water and tap with similar results. I have also tried different karo/water ratios with similar problems.

So what am I doing wrong? Do I expect too much mycellium? Or is there a trick I missed somewhere? When do other people use their jars if not when half full? How fast do your jars grow, and what are your mixtures?

thanx
d.k.


--------------------
     

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: simplemachine]
    #2096593 -

This is how I did this tek:

-Filled a jar with 150 ml of water, mixed with a tablespoon of Karo. Sterilized.
-Cut a small chunk off my fully colonized cake, dropped it in the jar, shook the jar very well.
-Sucked up some syringes of the mycelium water and directly injected it into my jars (didn't wait for mycelium to grow).
-After two days saw some slight growth. After a week my jars are half done (they are one pint jars filled with BRF and Verm).

This seems to be the easiest way. Also, I used the jar the colonized fastest for the chunks. Good luck.


--------------------
No question is so difficult to answer as that to which the answer is obvious.

Meow.

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: MycoCat]
    #2097183 -

Thanks MycoCat! I have a cake that's colonizing way ahead of schedule. I think I'll try your cloning method on him tonight or tommorrow. You should see this guy go! He's weeks ahead of jars germinated on the same day! Why so much variation between spores?


--------------------
     

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: simplemachine]
    #2097206 -

>Why so much variation between spores?


I think depending how old are those spores and where they coming from !

anybody else ?


--------------------

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: simplemachine]
    #2097424 -

Ive never had a jar more than a quater fill with actual mycelium.
A few clumps are all you need, as once shaken there broken into
many strands, the water only needs to suspend a few of these
strands for inoculation to work, but as is the case with spores,
the more the better.

Once the spores are germinated, its worth adding 1 or 2 drops of h2o2
to the jar, as when it breaks down, it adds o2 to the water, helping
the mycelium grow larger.


--------------------


Extras: Filter Print Post Top
Re: Liquid culture problems [Re: Paid]
    #2097562 -

this (h2o2) is the best tip you'r getting :wink: 


--------------------
comario


"crusaders against emotional poverty"

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: simplemachine]
    #2097745 -

your recipe sounds about right .
i use 100ml spring water 1 Tsp. Karo, in a 1/2 pint jar .
amount of mycelium varies by species/strain, usually a ball about the size of a quarter, within 5 days after germination .
shake the jar once a day to airate the culture .
dextrose and light malt extract work better . (jmo)


Extras: Filter Print Post Top
Re: Liquid culture problems [Re: deanofmean]
    #2097762 -

Cool guys thanxz! I guess I was expecting too much mycellium!!


--------------------
     

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: simplemachine]
    #2098695 -

Any other LM tricks I should know?


--------------------
     

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: deanofmean]
    #2098782 -

deanofmean said:
dextrose and light malt extract work better . (jmo)

 


how much malt extract  would you suggest? :grin: 


--------------------
"Why don't they make the whole plane out of that black box stuff."
Steven Wright.

Some say the glass is half empty, some say the glass is half full, I say, are you going to drink that?

"I'm desperately trying to figure out why kamikaze pilots wore helmets."

Extras: Filter Print Post Top
Re: Liquid culture problems [Re: Suby]
    #2098894 -

the same amount, about 4% .
Liquid Growth Medium

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* liquid culture using h2o2 baja 9,940 14 03/09/17 10:00 PM
by 3B3B4E
* Pan cyan in petri dish plus giant pan & liquid culture memberjockey 1,693 4 03/02/18 10:09 AM
by meltdowner
* 1st Liquid Culture, arrrgh! Seril 15,576 10 02/28/06 08:35 PM
by Limelight_Liver
* Starting a simple liquid culture method Garret_Hill 9,964 3 03/11/02 09:49 AM
by YourHighness
* Liquid Medium trouble (extracting Psylocibin ) CubensisMage 1,702 4 10/11/01 12:59 PM
by Workman
* Liquid Culture injection problem Bloomer69 2,199 5 03/14/03 01:48 AM
by BikeCourier
* Best liquid culture medium? lemunhed 7,113 4 06/09/03 05:06 PM
by lemunhed
* Easy Liquid Culture Container Tek SixTango 21,755 7 01/17/12 04:59 PM
by dubyastep

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,512 topic views. 10 members, 1,602 guests and 126 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.012 seconds on 16 queries.