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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Malt Extract, Vermiculite

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Invisiblesimplemachine
Manfly
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Registered: 09/14/03
Posts: 1,981
Liquid culture problems
    #2096563 - 11/12/03 11:45 AM (13 years, 26 days ago)

I've been working with liquid culture following this recipe...

50cc of water and 1/2 teaspoon karo syrup in a jar. This is mixed up sterilized for 10 min in boiling water, and innoculated.

When incubated at apx 85f I have germination and mycellial threads within 3-4 days which congeal into floating clumps over about 2 weeks.

My problem is that the jars just don't seem to fill up like I'd expect them to. I have heard that a liquid culture should be used when the mycellium fills half the jar. This is WAY under what I get. I have tried both distilled water and tap with similar results. I have also tried different karo/water ratios with similar problems.

So what am I doing wrong? Do I expect too much mycellium? Or is there a trick I missed somewhere? When do other people use their jars if not when half full? How fast do your jars grow, and what are your mixtures?

thanx
d.k.


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OfflineMycoCat
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Registered: 10/09/03
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Re: Liquid culture problems [Re: simplemachine]
    #2096593 - 11/12/03 11:52 AM (13 years, 26 days ago)

This is how I did this tek:

-Filled a jar with 150 ml of water, mixed with a tablespoon of Karo. Sterilized.
-Cut a small chunk off my fully colonized cake, dropped it in the jar, shook the jar very well.
-Sucked up some syringes of the mycelium water and directly injected it into my jars (didn't wait for mycelium to grow).
-After two days saw some slight growth. After a week my jars are half done (they are one pint jars filled with BRF and Verm).

This seems to be the easiest way. Also, I used the jar the colonized fastest for the chunks. Good luck.


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Invisiblesimplemachine
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Re: Liquid culture problems [Re: MycoCat]
    #2097183 - 11/12/03 02:47 PM (13 years, 26 days ago)

Thanks MycoCat! I have a cake that's colonizing way ahead of schedule. I think I'll try your cloning method on him tonight or tommorrow. You should see this guy go! He's weeks ahead of jars germinated on the same day! Why so much variation between spores?


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Offlinediacamomo
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Re: Liquid culture problems [Re: simplemachine]
    #2097206 - 11/12/03 02:51 PM (13 years, 26 days ago)

>Why so much variation between spores?


I think depending how old are those spores and where they coming from !

anybody else ?


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OfflinePaid
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Re: Liquid culture problems [Re: simplemachine]
    #2097424 - 11/12/03 03:33 PM (13 years, 26 days ago)

Ive never had a jar more than a quater fill with actual mycelium.
A few clumps are all you need, as once shaken there broken into
many strands, the water only needs to suspend a few of these
strands for inoculation to work, but as is the case with spores,
the more the better.

Once the spores are germinated, its worth adding 1 or 2 drops of h2o2
to the jar, as when it breaks down, it adds o2 to the water, helping
the mycelium grow larger.


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Offlinecomario2
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Re: Liquid culture problems [Re: Paid]
    #2097562 - 11/12/03 04:03 PM (13 years, 26 days ago)

this (h2o2) is the best tip you'r getting :wink: 


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Invisibledeanofmean
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Registered: 12/07/02
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Re: Liquid culture problems [Re: simplemachine]
    #2097745 - 11/12/03 04:48 PM (13 years, 26 days ago)

your recipe sounds about right .
i use 100ml spring water 1 Tsp. Karo, in a 1/2 pint jar .
amount of mycelium varies by species/strain, usually a ball about the size of a quarter, within 5 days after germination .
shake the jar once a day to airate the culture .
dextrose and light malt extract work better . (jmo)



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Invisiblesimplemachine
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Re: Liquid culture problems [Re: deanofmean]
    #2097762 - 11/12/03 04:52 PM (13 years, 26 days ago)

Cool guys thanxz! I guess I was expecting too much mycellium!!


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Invisiblesimplemachine
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Re: Liquid culture problems [Re: simplemachine]
    #2098695 - 11/12/03 09:25 PM (13 years, 26 days ago)

Any other LM tricks I should know?


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OfflineSuby
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Re: Liquid culture problems [Re: deanofmean]
    #2098782 - 11/12/03 09:46 PM (13 years, 26 days ago)

Quote:

deanofmean said:

dextrose and light malt extract work better . (jmo)

 




how much malt extract  would you suggest? :grin: 


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"Why don't they make the whole plane out of that black box stuff."
Steven Wright.

Some say the glass is half empty, some say the glass is half full, I say, are you going to drink that?

"I'm desperately trying to figure out why kamikaze pilots wore helmets."


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Invisibledeanofmean
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Re: Liquid culture problems [Re: Suby]
    #2098894 - 11/12/03 10:17 PM (13 years, 26 days ago)

the same amount, about 4% .
Liquid Growth Medium


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Malt Extract, Vermiculite

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