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Amazon Shop for: ½ Pint Jars, Agar, Rye Grain, Wild Bird Seed

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Invisibleagar
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GUNK IN LIQUID CULTURE
    #3963674 - 03/24/05 06:41 AM (11 years, 8 months ago)

Whatever you use, Karo, Dextrose, Honey, Light dry Malt, or any combinations - with water. You often get some caramelization in it - after PC?ing. The simplest way to acheive a clear liquid culture -without any gunk in it- is to simply PC it. Then, filter it through double coffee filters (twice), thus removing any gunk. Then??.PC IT AGAIN. Simple as that.



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OfflineFluxburn
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3963693 - 03/24/05 07:04 AM (11 years, 8 months ago)

Word I never have a problem with gunk bro. I need to get a stir plate thou and build some stir rods like u did since the stuff gets all chunky


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Invisibleagar
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Re: GUNK IN LIQUID CULTURE [Re: Fluxburn]
    #3963699 - 03/24/05 07:06 AM (11 years, 8 months ago)

>>>>>>>"chunky"<<<<<<<<<< ?

More like STRINGY, but I get the picture.


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OfflineIGnosticAbhorI
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3963771 - 03/24/05 08:03 AM (11 years, 8 months ago)

Hey Agar....Who are those two peeps in your sig? PM it to me if not here :smirk:

-Gnostic


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OfflineKulTiVater
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3963844 - 03/24/05 08:48 AM (11 years, 8 months ago)

do you kno where i can find the liquid culture tek??


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InvisibleMrMaddHatter
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Re: GUNK IN LIQUID CULTURE [Re: IGnosticAbhorI]
    #3964498 - 03/24/05 01:16 PM (11 years, 8 months ago)

I would say its a psychedelic man looking for a psychedelic woman :tongue:

Nice post agar!


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OfflineHotnuts
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
    #3964553 - 03/24/05 01:30 PM (11 years, 8 months ago)

Liquid cultures are easy as pie. Myself, I like to use light Karo corn syrup. I use a 1qt jar filled 1/3 of the way up with h20. Add a couple pieces of broken glass or a couple marbles in the jar to help break up the tissue later on when swirling. Then simply add 2-3 tablespoons of LIGHT corn syrup (Karo). Stir it up until the solution becomes clear and add you filtered lid of choice. Put a piece of foil over the lid. Pc for 25 minutes @ 15 psi. Done!


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OfflineKulTiVater
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3966822 - 03/24/05 11:38 PM (11 years, 8 months ago)

what are you supposed to do with it when you use it for cultivation?


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Invisibleagar
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
    #3966829 - 03/24/05 11:39 PM (11 years, 8 months ago)

Quote:

KulTiVater said:
what are you supposed to do with it when you use it for cultivation?




Do with WHAT? :confused:


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Offlinedr0mni
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3966859 - 03/24/05 11:49 PM (11 years, 8 months ago)

so the how will the gunk affect the culture? I think I heard someone saying something about caramelization causing mutants...

what's the deal?


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Invisibleagar
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Re: GUNK IN LIQUID CULTURE [Re: dr0mni]
    #3966998 - 03/25/05 12:26 AM (11 years, 8 months ago)

You want a CLEAR liquid culture, so you can see into & through it.

Meaning, while colonizing......if it contam's.... you will see something other than healthy myc in it.

Gunk in it, doesnt look like healthy Myc.


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OfflineKulTiVater
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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3967041 - 03/25/05 12:38 AM (11 years, 8 months ago)

the liquid stuff is that what the mushroom grow from or what?


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Invisibleagar
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
    #3967178 - 03/25/05 01:13 AM (11 years, 8 months ago)

Quote:

KulTiVater said:
the liquid stuff is that what the mushroom grow from or what?




Click  RIGHT HERE & READ.

agar.....:confused:why some folks don't research :rolleyes:


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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3967333 - 03/25/05 01:54 AM (11 years, 8 months ago)

that isnt exact about what you are supposed to do i have read tha b4 i was just asking how do you fruit it says nothing about it.


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Invisibleagar
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
    #3967355 - 03/25/05 02:02 AM (11 years, 8 months ago)

You "inoculate" (agar:bow:ANNO:bow:) a liquid culture into jars, or bags of wbs/rye/grain spawn. It colonizes VERY QUICKLY. Then, spawn that to bulk substrate, incubate/colonize, case & fruit out. :wink:


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Re: GUNK IN LIQUID CULTURE [Re: agar]
    #3967819 - 03/25/05 09:44 AM (11 years, 8 months ago)

never mind i kno what you mean now i had to read a couple times but i getit still tight tho.so you just pour it in the substrate?


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Invisiblepoboy
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Re: GUNK IN LIQUID CULTURE *DELETED* [Re: KulTiVater]
    #3967840 - 03/25/05 10:01 AM (11 years, 8 months ago)

Post deleted by poboy

Reason for deletion: xxx



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OfflineKulTiVater
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Re: GUNK IN LIQUID CULTURE [Re: poboy]
    #3967845 - 03/25/05 10:05 AM (11 years, 8 months ago)

ight cool
thanks


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Offlinenambi
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
    #3968005 - 03/25/05 11:24 AM (11 years, 8 months ago)

Wow this sounds really cool and I haven't even tried it yet. It seems much easier then making spore syringes! and from what I read this could knock off a week in colonizing time. I gotta try this thanks.


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Offlinenambi
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Re: GUNK IN LIQUID CULTURE [Re: nambi]
    #3984291 - 03/29/05 07:07 AM (11 years, 8 months ago)

How long does this culture last for?


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Amazon Shop for: ½ Pint Jars, Agar, Rye Grain, Wild Bird Seed

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