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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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 GUNK IN LIQUID CULTURE 
#3963674 - 03/24/05 04:41 AM (19 years, 27 days ago) |
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Whatever you use, Karo, Dextrose, Honey, Light dry Malt, or any combinations - with water. You often get some caramelization in it - after PC?ing. The simplest way to acheive a clear liquid culture -without any gunk in it- is to simply PC it. Then, filter it through double coffee filters (twice), thus removing any gunk. Then??.PC IT AGAIN. Simple as that.
 
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 Fluxburn
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Registered: 10/22/04
Posts: 2,216
Loc: Oakland, CA, USA 
Last seen: 13 years, 4 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3963693 - 03/24/05 05:04 AM (19 years, 27 days ago) |
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Word I never have a problem with gunk bro. I need to get a stir plate thou and build some stir rods like u did since the stuff gets all chunky
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ABSTRACT ART (Mine) http://nathanbelomy.com
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GUNK IN LIQUID CULTURE [Re: Fluxburn]
#3963699 - 03/24/05 05:06 AM (19 years, 27 days ago) |
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>>>>>>>"chunky"<<<<<<<<<< ?
More like STRINGY, but I get the picture.
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
Posts: 4,899
Last seen: 10 months, 21 days
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3963771 - 03/24/05 06:03 AM (19 years, 27 days ago) |
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Hey Agar....Who are those two peeps in your sig? PM it to me if not here 
-Gnostic
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KulTiVater
Comfortably Numb
Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3963844 - 03/24/05 06:48 AM (19 years, 27 days ago) |
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do you kno where i can find the liquid culture tek??
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MrMaddHatter
Dementia praecox
Registered: 06/07/02
Posts: 1,420
Loc: Everywhere
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I would say its a psychedelic man looking for a psychedelic woman 
Nice post agar!
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Hotnuts
old hand
Registered: 02/26/05
Posts: 3,436
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
#3964553 - 03/24/05 11:30 AM (19 years, 27 days ago) |
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Liquid cultures are easy as pie. Myself, I like to use light Karo corn syrup. I use a 1qt jar filled 1/3 of the way up with h20. Add a couple pieces of broken glass or a couple marbles in the jar to help break up the tissue later on when swirling. Then simply add 2-3 tablespoons of LIGHT corn syrup (Karo). Stir it up until the solution becomes clear and add you filtered lid of choice. Put a piece of foil over the lid. Pc for 25 minutes @ 15 psi. Done!
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KulTiVater
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Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3966822 - 03/24/05 09:38 PM (19 years, 27 days ago) |
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what are you supposed to do with it when you use it for cultivation?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
#3966829 - 03/24/05 09:39 PM (19 years, 27 days ago) |
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Quote:
KulTiVater said:
what are you supposed to do with it when you use it for cultivation?
Do with WHAT? 
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dr0mni
My Own Messiah
Registered: 08/21/04
Posts: 2,921
Loc: USF Tampa, Fl
Last seen: 16 years, 10 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3966859 - 03/24/05 09:49 PM (19 years, 27 days ago) |
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so the how will the gunk affect the culture? I think I heard someone saying something about caramelization causing mutants...
what's the deal?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
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Re: GUNK IN LIQUID CULTURE [Re: dr0mni]
#3966998 - 03/24/05 10:26 PM (19 years, 27 days ago) |
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You want a CLEAR liquid culture, so you can see into & through it.
Meaning, while colonizing......if it contam's.... you will see something other than healthy myc in it.
Gunk in it, doesnt look like healthy Myc.
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KulTiVater
Comfortably Numb
Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3967041 - 03/24/05 10:38 PM (19 years, 27 days ago) |
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the liquid stuff is that what the mushroom grow from or what?
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
#3967178 - 03/24/05 11:13 PM (19 years, 27 days ago) |
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Quote:
KulTiVater said:
the liquid stuff is that what the mushroom grow from or what?
Click RIGHT HERE & READ.
agar.....why some folks don't research 
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KulTiVater
Comfortably Numb
Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3967333 - 03/24/05 11:54 PM (19 years, 27 days ago) |
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that isnt exact about what you are supposed to do i have read tha b4 i was just asking how do you fruit it says nothing about it.
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
#3967355 - 03/25/05 12:02 AM (19 years, 27 days ago) |
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You "inoculate" (agar ANNO) a liquid culture into jars, or bags of wbs/rye/grain spawn. It colonizes VERY QUICKLY. Then, spawn that to bulk substrate, incubate/colonize, case & fruit out. 
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KulTiVater
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Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: agar]
#3967819 - 03/25/05 07:44 AM (19 years, 26 days ago) |
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never mind i kno what you mean now i had to read a couple times but i getit still tight tho.so you just pour it in the substrate?
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poboy
On the bounce
Registered: 03/08/05
Posts: 1,355
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Re: GUNK IN LIQUID CULTURE *DELETED* [Re: KulTiVater]
#3967840 - 03/25/05 08:01 AM (19 years, 26 days ago) |
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Post deleted by poboyReason for deletion: xxx
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Burn the land and boil the sea but you can't take the sky from me.     
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KulTiVater
Comfortably Numb
Registered: 02/22/05
Posts: 474
Loc: Wonderland
Last seen: 6 years, 2 months
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Re: GUNK IN LIQUID CULTURE [Re: poboy]
#3967845 - 03/25/05 08:05 AM (19 years, 26 days ago) |
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ight cool
thanks
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nambi
journeyman
Registered: 10/18/02
Posts: 132
Last seen: 13 years, 9 months
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Re: GUNK IN LIQUID CULTURE [Re: KulTiVater]
#3968005 - 03/25/05 09:24 AM (19 years, 26 days ago) |
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Wow this sounds really cool and I haven't even tried it yet. It seems much easier then making spore syringes! and from what I read this could knock off a week in colonizing time. I gotta try this thanks.
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nambi
journeyman
Registered: 10/18/02
Posts: 132
Last seen: 13 years, 9 months
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Re: GUNK IN LIQUID CULTURE [Re: nambi]
#3984291 - 03/29/05 05:07 AM (19 years, 22 days ago) |
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How long does this culture last for?
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