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Amazon Shop for: ½ Pint Jars, Agar, Air Pump, HEPA Filter, Petri Dish

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Offlinescatmanrav
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Gas Exchange for Liquid Cultures
    #3555290 - 12/29/04 01:36 AM (11 years, 11 months ago)

Wanted to find out what people think about how much gas exchange liquid cultures need. I got some peircable membranes and I'm guessing just putting one in with no other holes and putting the lid on normally would seal it and create a vaccum and not allow for gas exchange (bad). What I want to know is do I need to affix a filter to lids? It would be easier if not and I could just flip the lids over so the rubber doesn't seal. That wouldnt allow for much gas exchange but there isnt so much myc in there anyway..probably stops, or slows, creation of co2 once in the fridge too which is most of its life...

And I know I can affix some tyvek to lids, it would just be easier if I could just flip the lids, this is why I'm asking.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinejackel235
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Registered: 11/21/04
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Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3555400 - 12/29/04 02:03 AM (11 years, 11 months ago)

u know i just can't ever get my karo to work and don't know why


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Offlinedjred
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Registered: 08/27/04
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Re: Gas Exchange for Liquid Cultures [Re: jackel235]
    #3555414 - 12/29/04 02:06 AM (11 years, 11 months ago)

what? how can you not get karo to work..?

how long do you cook your jars how much heat flame? how much karo.cause really water doesn't really make any diff no matter what anyone says..you can't dump 2tbl of karo in a 50ml bottle or nothing,
but like i do i just pour some water about a half pint worth add some karo not measuring..boom with in a week or so i have agood karo jar. im still working with my karo from late nov. not to say my suggestions are by no means the way you should take for your own production. karo is so simple, a fucking monkey could do it.


Edited by djred (12/29/04 02:09 AM)


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Invisibleagar
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Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3555439 - 12/29/04 02:13 AM (11 years, 11 months ago)

Air/Gas exchange makes for thick healthy liquid cultures. However, it is a PITA, not getting any filter WET, which can QUICKLY lead to CONTAM'S wicking in :mad2:



I finally figured out a way to overcome that problem.


That is an IV "T" valve, with a tiny hepa grade iV filter attached to one side & a cap on top.

Flip switch one way (to filter) & it allows gas exchange. Flip the switch the other way & it close access to the filter & will not allow it to get WET.

When the filter is closed OFF, it allows interior access - with syringe needle, after you unscrew the CAP.

If you know a surgical nurse, she can bag you a few of these. If not, they are available at surgery & medical supply houses.

Lid, T valve & filter will withstand PC'ing.

To install, simply drill hole in cap, fit a rubber grommet into cap hole, force one end of T valve through hole in grommet.


--------------------


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Offlinedjred
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Registered: 08/27/04
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Re: Gas Exchange for Liquid Cultures [Re: agar]
    #3555453 - 12/29/04 02:17 AM (11 years, 11 months ago)

agar your creative...from what i can see from your post you have the resources to collect stuff and make use of it. i assume your an older man.given by your post.. i don't recal to many younger adults creating iv t valve gadgets to go on top of thier karo jars..:)


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Offlinejackel235
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Re: Gas Exchange for Liquid Cultures [Re: djred]
    #3555477 - 12/29/04 02:27 AM (11 years, 11 months ago)

well i use 100ml of water 1 teaspoon of karo 1 cc of spores and pc it to sterlize and then i make it air tight maybe cause of airexchange i don;t know my jars like 3 weeks old and nothing inthem


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Offlinescatmanrav
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Re: Gas Exchange for Liquid Cultures [Re: djred]
    #3555484 - 12/29/04 02:29 AM (11 years, 11 months ago)

Interesting, so you dont think it will work just flipping the lids over? Thats a handy little gadget and thats one reason I wanted to forgo the filters...tyvek always gets wet and dries quickly but then sticks to the lid and such..just a pain...Well I may end up trying one out with the lid flipped over cause that looks like a bit of a build and probably tough to find (not sure of any medical supply places around and a look in the yellow pages gets me nothing, know no nurses either)...though I could maybe purchase online I'd still like to do it simply if I can.

Hey don't they make those for air pumps for fish too? Maybe I could find some at a pet store? Well thanks..I'm still interested in any replies from anyone who may have had success just flipping the lid..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinedjred
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Registered: 08/27/04
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Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3555543 - 12/29/04 02:44 AM (11 years, 11 months ago)

wow scat you have managed almost 3,000 post since may..


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Invisibleagar
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Re: Gas Exchange for Liquid Cultures [Re: djred]
    #3555549 - 12/29/04 02:45 AM (11 years, 11 months ago)

Well.... I'm no pup. But, anyone over 18, with a few bucks, a smile & gift of gab.... can round this junk up






One gallon size - with stir-bar inside.


Half gallon size with injection / asperation resealable port on side


--------------------


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Gas Exchange for Liquid Cultures [Re: djred]
    #3555565 - 12/29/04 02:51 AM (11 years, 11 months ago)

2,000 of them since september too..thats when I really started posting..I lurked and read for a little white Then September came and that was almost 2 months after I started growing so I started learning alot quickly at that point..I try and go through just about every post here...I tend to ramble alot too so I post on alot of them.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedjred
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Registered: 08/27/04
Posts: 922
Last seen: 8 years, 3 months
Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3555574 - 12/29/04 02:53 AM (11 years, 11 months ago)

lol agar...man i have just a basic half pinter with a medical tape covering holes with some tvek


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Gas Exchange for Liquid Cultures [Re: agar]
    #3555586 - 12/29/04 02:57 AM (11 years, 11 months ago)

Quote:

agar said:
, anyone over 18, with a few bucks, a smile & gift of gab....





You forgot some ambition..something most younger people lack..I think thats what makes you seem older, whether you are or not.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
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Re: Gas Exchange for Liquid Cultures [Re: djred]
    #3555607 - 12/29/04 03:11 AM (11 years, 11 months ago)

Milipore & Transpore medical tape is good stuff. I also use it to seal petri's. Hell, a half pint works. Nothing wrong with that.


--------------------


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Invisibleagar
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Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3555620 - 12/29/04 03:19 AM (11 years, 11 months ago)

Quote:

scatmanrav said:
Quote:

agar said:
, anyone over 18, with a few bucks, a smile & gift of gab....





You forgot some ambition..something most younger people lack..I think thats what makes you seem older, whether you are or not.




Patience & tenacity are important, Set a goal, go for it & don?t stop until you reach it. :wink:


--------------------


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Offlinehyphae
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Re: Gas Exchange for Liquid Cultures [Re: agar]
    #3556339 - 12/29/04 08:38 AM (11 years, 11 months ago)

I'll tell you all I have ever done with Karo was to use unmodded lids tighten and shake then loosen again without a problem. KISS works for me :wink: Just another option :laugh:


--------------------
Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"


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Offlinescatmanrav
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Registered: 05/08/04
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Re: Gas Exchange for Liquid Cultures [Re: hyphae]
    #3557167 - 12/29/04 02:04 PM (11 years, 11 months ago)

Really so no holes or filters in the lid and even on with the ruberside down? This is good news to my experiment.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetheknighterrant
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Re: Gas Exchange for Liquid Cultures [Re: scatmanrav]
    #3557416 - 12/29/04 03:06 PM (11 years, 11 months ago)

might be talking out of my ass here, correct me if i am wrong. i can't see a liquid culture needing a great amount of air transfer since 1: there will be oxygen dissolved in the water and 2: CO2 amounts produced by the culture will be nowhere near those of fruiting bodies. i will agree that the more oxygen that is available to the culture the better and the lowest CO2 level possible can't hurt, but for most people i would think that 1/8 inch hole in the lid with an appropriate filter would be sufficient.
this is what i have been doing with my cultures (not that i have been doing this long mind you), and using fabric bandaids for filter. Bandaids because: they come out of the packaging sterile, the allow for low contaminate free air transfer, and the fabric ones stick nicely even through the pc'ing process. so far, contaminate free jars and cultures. kind of ghetto tek but seems to work fine.

agar, love your t valve. that is so cool.

tke


--------------------
The oldest and strongest emotion of man is fear. The oldest and strongest kind of fear is fear of the unknown.
--H.P. Lovecraft

Demented Piper Press


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Offlinescatmanrav
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Re: Gas Exchange for Liquid Cultures [Re: theknighterrant]
    #3557448 - 12/29/04 03:19 PM (11 years, 11 months ago)

Quote:

theknighterrant said:
might be talking out of my ass here, correct me if i am wrong. i can't see a liquid culture needing a great amount of air transfer since 1: there will be oxygen dissolved in the water and 2: CO2 amounts produced by the culture will be nowhere near those of fruiting bodies. i will agree that the more oxygen that is available to the culture the better and the lowest CO2 level possible can't hurt, but for most people i would think that 1/8 inch hole in the lid with an appropriate filter would be sufficient.
this is what i have been doing with my cultures (not that i have been doing this long mind you), and using fabric bandaids for filter. Bandaids because: they come out of the packaging sterile, the allow for low contaminate free air transfer, and the fabric ones stick nicely even through the pc'ing process. so far, contaminate free jars and cultures. kind of ghetto tek but seems to work fine.

agar, love your t valve. that is so cool.

tke




All of this sounds right..thats what I do as well but I use tyvek as well..bandaids to cover needle holes in the tyvek...but now I'm trying to simplify even further and remove all holes from the lid..except one I put in for the membrane.

I'll be trying it out and posting my results back..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Agar, Air Pump, HEPA Filter, Petri Dish

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