Condensation formed inside the plate then dripped onto the agar....any problems with this?

Sorry, I don't know how long you can save agar dishes. But since it is fertile conditions for mold and bacteria, I wouldn't chance it after too long.

Colonization should take 4 to 5 days. Did you add the spores to the center or multiple spots in the dish? Use the mycelium closest to the center (away from the sides) to avoid contamination when you take the samples to innoculation your substrate (rice cakes).

Check out your mycelium under a microscope. It's kewl!

Tor

I've noticed that if one injects a small drop of spore juice onto a dish it will take about 2 days to show signs of growth and about 10-13 days for complete colonization. Spore print scrapings take slightly longer.

Thanks man. That helps! I notice some growth today. I am incubating at 70-75 degrees.

I guess all I really needed to do was to wait till the agar set then turn the plates upside down? Maybe I should be incubating that way also?

Later, Tor

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