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Offlineshroomeritus
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Registered: 12/18/00
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Agar - Will a fully colonized plate be a problem?
    #340421 - 06/14/01 06:34 AM (17 years, 4 months ago)

Hi everyone,
One of my plates have been completely colonized with rhizomorphic mycelium. In TMC, it says that a plate should only be colonized 2/3 ( or 3/4 ) of the way. Otherwise, contams from the sides of the petri could inhabit the mycelium and go undected.

My question is:
Would cutting only the mycelium that is 3/4 of the way away from the edge work to only get clean agar? + Would it matter if I was using Peroxidated Agar ?



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Offlineegghead
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Re: Agar - Will a fully colonized plate be a problem? [Re: shroomeritus]
    #340438 - 06/14/01 07:29 AM (17 years, 4 months ago)

Cutting from the center will work fine. Also, peroxidated agar is no good for germination. You can use it w/ clones and isolates, but I wouldn't do it unless it's necessary.



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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