Whyterye said:

If you have a plate that doesn't look great should you still take transfers or start back from spores? can you bring a bad transfer back from the dead or does it take starting over?

---

seand04 said:

With the agar plates will grow this exact isolated genetics?

---

MLPismyOPSEC said:

seand04 said:

With the agar plates will grow this exact isolated genetics?

---

No, not exactly. You'll likely get most of the phenotype, but not all because there are simply too many "branches" (for lack of better term) in the mycelium to get those narrowed down. Picture a pie graph, with thousands of tiny slivers each representing unique phenotypes. When you take your first clone, as you just did, that's cutting the pie into a single slice. Now that slice still has a couple hundred slivers, but far fewer than the whole pie. So you can continue to clone and narrow down the genetic pool, and that will get you closer to those exact genetics, but it'll take some time.

---

tydygy said:

So I did a bit of agar work this weekend. I decided to test some variations. FOR SCIENCE!

I did some glass(pyrex), some Celtreat and some Alien Holy Grails(pp5). I tried to use Cap's SAGARI method to see if I like soft liquifiable agar when shaking my grains. None of them are pre-pours. (Sorry Alien) Oh yeah, and I did some with a crap ton of food color and some with none.



So far I hate dropping pyrex on a tile floor with no shoes on. That sucked and lost me 1 & 1/2 dishes (saved one of the lids). The HGs and Pyrex came out of the PC a bit wet, so I let them sit in the oven at 200f for about 45 minutes before moving them to the SAB. After pouring Saturday evening, letting them sit in the SAB overnight and doing a few transfers Sunday, you can see that the HGs and Pyrex are clear and the Celtreats have condensation. I used a water bath to get the agar bottles to 45c before pouring, but maybe you need to go cooler for Cap's reduced agar recipe?

Anyhow, so far for ease I like pouring the HGs. Less condensation and no fucking around with saran wrap or tape. I set plenty more of each dish aside to watch for contamination. I guess we will see what happens.

---

Hide said:

I first got into this hobby with MSS to brown rice.
Then I tried agar and wasn't 100% if I was doing it right, had some funny looking plates.

I didn't bother for a while as my plates never looked as good as the ones online so I stashed them all away.

I recently found the plates, many of them survived. Clones to transfers to 2nd transfers survived. I then gave it another go to take one of 2nd transfer and do a 3rd but got green nasties. Looks easily recoverable if peroxide will work. How effective is 3% solution? Pour onto plate or cotton bud?

Making a bigger SAB tomorrow.. Will definitely help.


Cookie rack or no rack? Towel or no towel? Bleach water or warm soap to settle air? I used a mini kitchen gas jet with the blue flames to sterilise my scalpel but not sure if it got hot enough. It got red but would fade away quick.

I just want to get this shit right. I've ordered dextrose too to try LC. Any tips or sterile technique tips would be great. Arm hole cover recommendations? Any order of spraying and not spraying? Thank you

---

Don’t waste time with peroxide. Just practice transfers by . . . Making transfers. . . You can transfer off a dirty dish and have plenty of success. . . Just don’t transfer the contams. . .

Rack yes as gravity is your friend concentrating comtams on the bottom of your SAB.
Towel personal preference, I’d suggest placing your rack higher rather than a towel. . .

Gravity to settle air. . . Before getting a hood, I left my SAB setup, and never sprayed walls or roof (thanks mushboy for confirming my madness that dry works fine). . .

Red = red, so is hot enough. . .

If ya can’t do agar, LC will make you abandon all hope. . . Practice agar. . . Then practice some more.

Any fabric will work as a cover for arm holes. . .

My tips:
Open petri as little as possible = work quickly through a ‘cracked’ dish, just enough to get the scalpel in and out. . .
Imagine a flow of dirty air travelling downward in your SAB, and keep things out of that flow where possible.
Wrap your petri from the moment they gel (solidify), and only unwrap when transferring. . . Then rewrap promptly. . .
Practice, practice & practice. . . It’ll come really quick. . .