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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26889690 -

Myc_Hunt, what recipe did you use?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ChRnZN]
    #26889863 -

Hey gang,

Not much on asking questions, but I’m hell on search and reading. I’m learning that’s for sure. Had enough success that I’m still trying.

Anywho, a B+ clone transfer that I want to keep growing.
I read here about transfers, take what interests you.
I like that.

But this ones got me slightly stumped. I like it all.
Damn green dots....

So could use some advice for where to cut...
Thanks and mush love......

Edited by UpRigger (08/20/20 12:51 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: UpRigger]
    #26889869 -

UpRigger said:
Hey gang,

Not much on asking questions, but I’m hell on search and reading. I’m learning that’s for sure. Had enough success that I’m still trying.

Anywho, a B+ clone transfer that I want to keep growing.
I read here about transfers, take what interests you.
I like that.

But this ones got me slightly stumped. I like that it all.
Damn green dots....

So could use some advice for where to cut...
Thanks and mush love......




In a SAB, Id try for the areas furthest from the contam. around 12 o'clock area


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Re: AGAR ENVY! (Anything and All things agar!) [Re: UpRigger]
    #26889872 -

Also I try to keep my transfer rice size or smaller, but I’ve always cut against the grain, so to speak. Can I cut length wise, like trying to isolate single strands of myc...
Thanks....

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Re: AGAR ENVY! (Anything and All things agar!) [Re: UpRigger]
    #26889887 -

You can. People use punches, cuticle files, heart shaped tools ect.
I would just aim for the leading edge on that growth up top. The contam is likely from you, so just be sure to be aware of your hand placement when making the xfer. I would rotate that place 180 and have the section you are going to xfer from closest to you

careful with how you handle that plate as well. it looks like the mold is sporulating and you dont want to further compromise the cube myc


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Camera93]
    #26889906 -

Thanks......

And I know it’s from me.... darn it..✌🏻

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Re: AGAR ENVY! (Anything and All things agar!) [Re: UpRigger]
    #26890056 -

lol yeah not a coincidence there’s one in the exact same spot on each side.

I’m betting scalpel handle. At least that’s what it is for me usually.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ChRnZN]
    #26890461 -

ChRnZN said:
Myc_Hunt, what recipe did you use?


500ml water, 7.5g of lme and agar


--------------------
Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26890549 -

Is this mycelium? or mold or both


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Quadryder]
    #26890553 -

So I just made a couple agar plate using a couple fat PE. With the agar plates will grow this exact isolated genetics?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Quadryder]
    #26890555 -

Looks like mycelium to me


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26890581 -

Those are huge tissue samples


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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #26890616 -

Genghis Chron said:
Those are huge tissue samples


Is that bad? I was thinking it might help 🤷‍♂️

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26890734 -

Nah it should still work fine. I take the smallest sample I can with some tweezers. Usually about the same size as my agar transfers (grain of rice-ish).


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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Fresh Prints]
    #26890785 -

Genghis Chron said:
Nah it should still work fine. I take the smallest sample I can with some tweezers. Usually about the same size as my agar transfers (grain of rice-ish).


Awesome,  I just cut out the pieces with a flamed exacto knife.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26890797 -

I’m all about small transfers too.

lol I love cloning huge stems like that, makes it so easy to get a clean piece.

I cloned one similar to those but shorter, it looked like a cupcake. Didn’t have a huge pinset but put out a lot of weight. Rock hard solid fruits all funky lookin.



That somehow ended up being about 400g wet.


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LAGM2020

Edited by A.k.a (08/20/20 11:27 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26890852 -

They look awesome. :rockon: I just ordered this pack of petri dishes, Wondering what is the best way to make them. Make agar and sterilize it then pour, or pour them then PC the dishes?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26890864 -

You cannot PC polystyrene. If you want to pre-pour you need PP5 plastic. With those dishes you'll want to pour your sterile media in a SAB or in front of a flow hood 👍

Edit: Check out Bod's Comprehensive Agar Tek


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Edited by The Fresh Prints (08/21/20 12:48 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: seand04]
    #26890890 -

Nichrome said:
I've always personally found pouring and wrapping dishes with parafilm as soon as they're cool enough to be superior to any of the no pour methods I've used.

I can comfortably pour and wrap a few hundred plates in a day like that and have way more control over outcome.

Plates are cheap and pouring is really easy.


Plates are expensive. . . Cheap financially, expensive environmentally. . . Polystyrene is one or the worst plastics also. . .

I prefer post-pour (Aka pour) with glass also, a bit more of a hassle, yet much less condensation. The sous vide has reduced this remarkably as pouring at 46°C has made a huge difference, though glass are more susceptible to temp changes

seand04 said:
They look awesome. :rockon: I just ordered this pack of petri dishes, Wondering what is the best way to make them. Make agar and sterilize it then pour, or pour them then PC the dishes?


What genghis said, though make sure you pour the whole sleeve in one go. Once you open it, sterility is compromised. Pour them, wrap them, they will store for months :thumbsup:


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The Official Kiwi Cultivators Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #26890979 -

If you have a plate that doesn't look great should you still take transfers or start back from spores? can you bring a bad transfer back from the dead or does it take starting over?

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Shop: Sporeworks.EU Spores for European Microscopy   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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