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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26820853 -

D3monic said:
What kind of agar? I get little to no sediment in my plates with lme. Also once agar is PC I don’t swirl it or anything and leave the last 1/4 in the media bottle. That way any sediment that may be present stays in the bottom and not on a plate


grain water, oats specifically. plus, thinking about ordering the charcoal powder that you recommended because apparently the activated carbon that i bought is different? i have always thought that they are both the same thing... the one i bought is used for aquariums so im not sure why it doesnt work. i ran a test and when im mixing it, the water turns black but once i stop, the sediments settle at the bottom and the water turns clear/grey tint. it doesnt seem to dissolve and turn the water black

     

Edited by ouuwee (07/13/20 12:59 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ouuwee]
    #26820863 -

I like sediment. It gives me some contrast when looking at things up close under the scope. I've never seen it hinder or impede anything. That's just me though.



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Question everything, it's very responsive.

Culture Storage

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #26821142 -

Inthepit said:
Wooo hooo! checkout the rhizomorphic legs on that one!
And yes the more I tried to knock the wedges off the top,
the more stuck! :lol:


I have a wedge stuck to the top of a Petri dish and the myc somehow reached across to the agar and started growing.


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My first grow:
https://www.shroomery.org/forums/showflat.php/Number/26791232

Edited by Forevergreen (07/13/20 03:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #26821149 -

The Dalcassian said:
Quote:
C12ShroomMan said:cut a 1" piece off the roll to make a mini roll and its perfect.


How do you do this, I tried and I ended up with one raggedy ass end piece just frayed to shit. Nowhere near neat enough for my liking 😂
My knives are sharp too.


Just save up and get parafilm. I fucking love parafilm


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My first grow:
https://www.shroomery.org/forums/showflat.php/Number/26791232

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Forevergreen]
    #26821175 -

Forevergreen said:
The Dalcassian said:
Quote:
C12ShroomMan said:cut a 1" piece off the roll to make a mini roll and its perfect.


How do you do this, I tried and I ended up with one raggedy ass end piece just frayed to shit. Nowhere near neat enough for my liking 😂
My knives are sharp too.


Just save up and get parafilm. I fucking love parafilm


Wow! I just saw 76m of parafilm for £168 that more than £2 a meter.
Why is parafilm preferred over other grafting tapes? Or M3


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Here Lies This Individual




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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #26821280 -

Labgrade, allows the proper oxygenation of a sample, also has no odor and it's self sealing thermoplastic.

Basically it's lab approved, for lab work probably one of the few things you can use. Think I remember like a nescfilm or something that is interchangeable.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro] * 2
    #26821437 -



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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #26822010 -

The Dalcassian said:
Forevergreen said:
The Dalcassian said:
Quote:
C12ShroomMan said:cut a 1" piece off the roll to make a mini roll and its perfect.


How do you do this, I tried and I ended up with one raggedy ass end piece just frayed to shit. Nowhere near neat enough for my liking 😂
My knives are sharp too.


Just save up and get parafilm. I fucking love parafilm


Wow! I just saw 76m of parafilm for £168 that more than £2 a meter.
Why is parafilm preferred over other grafting tapes? Or M3


I just prefer parafilm over saran wrap and such.


--------------------
My first grow:
https://www.shroomery.org/forums/showflat.php/Number/26791232

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26822132 -

D3monic said:
Wish my SAT would look like that. Very weak looking. Need new print.


It was a numbers game. I made 7 streak plates and took 20+ transfers, this one was the beat but I want to say they about 60% of them looked average to questionable.  This and a few others look great though.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26822213 -

D3monic said:



Making me want to pull the trigger on another box of dishes and a hole punch :sunny:


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My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26822323 -

D3monic said:



Your agar plates are living art. I know that sounds cliche as fuck, but you moreso than others seem to kill it with the colors and rhizo spread.


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26822344 -

My secret is I only share the pretty ones :rofl2:


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D3’s Glog
D3's Agar TEK
Agar Porn Gallery
RW x MelMak Grow Along Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26822346 -

"If this guy only saw the 4,083 that look like absolute garbage" rofl


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #26822406 -

Post only the good and never talk about the bad.

I just threw out 4 plates that looked like I streaked the inside lip of a toilet. Can't for the life of me figure out what went wrong. Usually I know where/when I'm going to contam.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: oculodextro]
    #26822415 -

This is a new hobby for me, so any growth that looks alright is good enough for me


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26822481 -

D3monic said:
My secret is I only share the pretty ones :rofl2:


You're screwed now! You set the bar so high, maintaining those appearances is going to be a full-time job! :laugh2:




Damn it! I could have had a sterile print :lol:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MLPismyOPSEC]
    #26822724 -

Hello all, here to learn some Agar techniques and trying to solidify some mycology in my fat head. Did BRF cakes about 10 years ago and then again about 5 years ago with mediocre success. This looks a lot more fun and proficient than shooting for success with cakes, so here we go.

In mid June ordered all the Agar supplies, gathered up all my old syringes (and a new one) and emptied them out onto 20 plates. Was around 50/50 contaminated to not (at least visibly.) It was probably late to do the first transfer, but I was out of town for a while. I poured 60 new plates yesterday and transferred to the new plates (not all of them, 2/3 samples per original plates.) Thanks to all for the knowledge here, I'm on page 40 right now and plan on reading all the way up to here I find a lot of great info on every page almost.

Here are photos of my OG plates. What is up with my food coloring, that was 5 drops of red into 500ml of water whisked in pretty well, all sorts of weird streaking, in a couple plates the color dissipated all together. Also I got a bunch of bubbles on the first pour but not later ones. Also photos of the bad plates; should I try to transfer off of those at all?? The one with the black spot looks to have healthy growth, the others look mildly interesting to me.









Edited by Huldrelokk (07/14/20 12:46 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huldrelokk]
    #26822767 -

Wow brosiph. Those look good for plates inoculated with a syringe. Since you have so many decent germination points to choose from I don’t see any reason to mess with plates full of black, possibly deadly mold lol.

Myc eats the food coloring as it grows. Other stuff probably eats it as well. I’d take some transfers from the outer edge of a couple clean spots. I’d probably transfer into 4-5 new plates. Let those grow out. They should be clean but I’d still do one more round of transfers, but also drop a few wedges into grain just to get some jars started. 

If you transfer into like 20 plates now you’ll just end up with a ton of overgrown plates of plates that get transferred from and never used.


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Sour grapes, sweet revenge
Heaven starts right where hell ends

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Roger Clemency]
    #26822996 -

Roger Clemency said:
If you transfer into like 20 plates now you’ll just end up with a ton of overgrown plates of plates that get transferred from and never used.



Thanks for the advice! I really don't have a plan, I was figuring the more plates I do the more likely chance I will end up with something nice to put onto grain. Did you see any plates that are especially nicer looking? I won't touch the moldy ones, I have enough to throw them out. (Do you guys toss them straight into the trash?)

So now that I have 8 plates of t0. I made 2 transfers of each of those, so now have 8 t0 and 16 t1 plates. I won't make as many plates on my t2 transfers, but im curious what you guys do with all these older plates. How do you decide when you want to stop making new transfers?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Huldrelokk]
    #26823029 -

Huldrelokk said:
Roger Clemency said:
If you transfer into like 20 plates now you’ll just end up with a ton of overgrown plates of plates that get transferred from and never used.



Thanks for the advice! I really don't have a plan, I was figuring the more plates I do the more likely chance I will end up with something nice to put onto grain. Did you see any plates that are especially nicer looking? I won't touch the moldy ones, I have enough to throw them out. (Do you guys toss them straight into the trash?)

So now that I have 8 plates of t0. I made 2 transfers of each of those, so now have 8 t0 and 16 t1 plates. I won't make as many plates on my t2 transfers, but im curious what you guys do with all these older plates. How do you decide when you want to stop making new transfers?


Im no expert either but I usually start with oone or two dishes for spores to agar, then transfer out to 5 and maybe transfer those out to 10 or just use the. 5. Saves you a ton of wasted plates


--------------------
India Woodlover Growalong
Tweeq's Woodlovers Adventures
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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