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Forevergreen
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Help me on my First grow. Spores to agar to grain to monotubs
#26791232 - 06/29/20 02:02 AM (3 years, 6 months ago) |
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I made agar in tequila bottles at 15gr agar, 12 gr MLE and 750 ml water. I mixed those ingredients in a saucepan and brought to a boil. as soon as it came to boil you pour it into your bottles then pressure cooked at 15 PSI for 30 min. then used my sterilizer as a 120-130F waterbath.

I then poured plates and set hot water on top. The hot water did nothing. I let them sit for 24 hours with no parafilm in stacks and the condensation went away. However, before i used them in my SAB I sprayed with ISO and got condensation on some. Next time i will not spray my stacks with ISO before putting into SAB. I wiped each plate down and dried so it shouldn't be a problem.

I smoked some weed (legal state)

These are the leftover plates that will get wrapped and put into fridge

I used my SAB to inoculate at least 10 plates of: Amazon pes Hawaiian PES Red Boys Ecuadorian
Okay i still find it hard to do just one drop on each dish AND I CAN BARELY SEE IN MY SAB TRYING TO DO TWO SINGLE DROPS. am I the only one who is like "holy shit, this isnt easy!" I have to hunch down to see through my arm hole and try very careful not to squeeze too hard and im successful, but sometimes I get a squirt and I just opened another dish and tried to pour the extra into the other dish. Probably gonna contam, but fuck it. I also got two different syringes from this source and one was way easier than the other. Oh well. I try so hard to do a single drop haha.
Plates at 77.





All I will do over next few days is maybe remove parafilm to rid condensation, especially if it isn't gone by tomorrow I will carefully remove all parafilm and allow dishes to do their thing until moisture is gone then I'll replace parafilm
Edited by Forevergreen (07/19/20 03:13 PM)
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Rapjack
Oat Soakin' Toker


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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26791258 - 06/29/20 02:37 AM (3 years, 6 months ago) |
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Good job mate! Looking good. Visibility in a SAB can be a pain, I'd recommend getting a clamp lamp or LED shop light for sterile work. Game changer. I also used to elevate the entire table my SAB was on with 8" plastic bed risers which put it at around shoulder level while sitting. This allowed me to just stick my arms straight out with elbows sometimes resting on the edge of the table to work and do a lot less hunching. Good luck, and look forward to future progress!
Edited by Rapjack (06/29/20 02:44 AM)
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jcm4620
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Re: First grow. Spores to agar to grain to monotubs [Re: Rapjack]
#26791340 - 06/29/20 04:19 AM (3 years, 6 months ago) |
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just a lil trick i use to get rid of condensation on tops of my plates. il use a very very light heat source just above the lids of the plates.
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Roger Clemency
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Registered: 03/23/20
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26791465 - 06/29/20 06:44 AM (3 years, 6 months ago) |
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Just leaving them in stacks usually works for me. I’ll put an old plate or two up top and just let them take all the condensation.
Also you don’t need to store plates in the fridge, it’s actually better to leave them at room temp so that anything nasty shows itself before you go using that plate.
Spore prints are much easier to work with in my experience than spore syringes. I hate syringes. I’m hoping you get clean growth right out and you go on to grow many trippy mushrooms. But if you keep getting plates full of bacteria don’t get discouraged, just get a print. Starting with agar is the way to go though
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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jcm4620
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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26791630 - 06/29/20 08:41 AM (3 years, 6 months ago) |
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ya prints are way better 😃 il see if i have any cubes left when i get home from work tonite. if i do il send ya one. im just not sure if i have any cube prints left. i think all i have right now is some pan alabamas but il check for u. and if i dont im sure someone will be kind enough to send ya one
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metaphoric
Mr. Bater



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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26791642 - 06/29/20 08:50 AM (3 years, 6 months ago) |
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You can also germinate your syringe on some grain, then pluck a few individual grains out of the jar and put them on agar to get the culture cleaned up.
Pretty sure it was jcm who gave me the idea originally. Works great, I hate squirting a syringe onto an agar plate.
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AtmozFear
just a shade of myself


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Re: First grow. Spores to agar to grain to monotubs [Re: metaphoric]
#26791661 - 06/29/20 09:04 AM (3 years, 6 months ago) |
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more and more people are saying this... I'd like to know what the super-specialists think
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jcm4620
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Re: First grow. Spores to agar to grain to monotubs [Re: AtmozFear]
#26791689 - 06/29/20 09:24 AM (3 years, 6 months ago) |
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ya i do that if i have a hard time with germ on agar 😃
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Roger Clemency
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Re: First grow. Spores to agar to grain to monotubs [Re: AtmozFear]
#26791708 - 06/29/20 09:34 AM (3 years, 6 months ago) |
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Quote:
AtmozFear said: more and more people are saying this... I'd like to know what the super-specialists think
Disclaimer - all of the following have been forged. For entertainment purposes only.
Quote:
Pastywhyte said: Spore syringes make you gay. More so than just eating or growing mushrooms alone:
Quote:
cronicr said: Using spore syringes basically supports terrorism. How many middle eastern girls do you want to be maimed by your mush cult choices??
Quote:
fahtster said: Never use syringes to agar. Firstly it makes you irreversibly gay, and secondly it will lead to extremely low potency mushrooms...if they ever even germinate
Quote:
AtmozFear said: Roger Clemency is a d!ck and keeps misquoting people, sowing chaos and confusion
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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metaphoric
Mr. Bater



Registered: 05/18/15
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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26791718 - 06/29/20 09:39 AM (3 years, 6 months ago) |
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They're all talking about spawning the grain you noc up with a syringe. That's not what we're talking about.
I'm going to ignore the fact that they are fake quotes and address the point I think you're trying to make.
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Forevergreen
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Re: First grow. Spores to agar to grain to monotubs [Re: jcm4620]
#26791758 - 06/29/20 10:04 AM (3 years, 6 months ago) |
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Quote:
jcm4620 said: ya prints are way better 😃 il see if i have any cubes left when i get home from work tonite. if i do il send ya one. im just not sure if i have any cube prints left. i think all i have right now is some pan alabamas but il check for u. and if i dont im sure someone will be kind enough to send ya one
Wow that would be really cool. Not expecting anything here other than y'alls help.
I'm going to noc up these wide mouth pint jars, I just bought WBS with a bunch of cracked corn and my dumbass didn't notice til I got home because it didn't list it on the ingredients.. Im just gonna buy another bag and buy a bird feeder or something and use the old bag for the birds. I don't save receipts and take it as a lesson. Birds get fed now lol
The tractor supply store also sells oats for $15.. Debating doing just WBS or half jars WBS and other half Oats.
Edited by Forevergreen (06/29/20 10:06 AM)
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metaphoric
Mr. Bater



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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26791767 - 06/29/20 10:10 AM (3 years, 6 months ago) |
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Wbs has more inoculation points but oats are basically fool-proof. I love oats, got started with wbs first.
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AtmozFear
just a shade of myself


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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26791770 - 06/29/20 10:11 AM (3 years, 6 months ago) |
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your entertainment doesn't make any sense
Edited by AtmozFear (06/29/20 10:11 AM)
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Roger Clemency
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Registered: 03/23/20
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Re: First grow. Spores to agar to grain to monotubs [Re: AtmozFear]
#26791795 - 06/29/20 10:20 AM (3 years, 6 months ago) |
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It was more about not using syringes to agar then about germinating on grain and then going to agar for trouble syringes, which I guess is what you meant.
The OP is probably like wtf is going on I haven’t even had a problem yet. I’m just here trying to hate on syringes bro, don’t try to confound me.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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AtmozFear
just a shade of myself


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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26791799 - 06/29/20 10:22 AM (3 years, 6 months ago) |
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like i said would love to hear the experts on this...
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Roger Clemency
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Re: First grow. Spores to agar to grain to monotubs [Re: AtmozFear]
#26791886 - 06/29/20 11:18 AM (3 years, 6 months ago) |
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Lol about what though?
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Phrontist
Determined



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Re: First grow. Spores to agar to grain to monotubs [Re: Forevergreen]
#26791962 - 06/29/20 12:16 PM (3 years, 6 months ago) |
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Quote:
Roger Clemency said: Disclaimer - all of the following have been forged. For entertainment purposes only.
Homophobia isn’t terribly entertaining.
Quote:
Forevergreen said: am I the only one who is like "holy shit, this isnt easy!"
Someone please correct me if this is awful advice, but if you squirt a little on a piece of clean aluminum foil rather than directly onto the agar, you could wet your loop in the foil puddle and then put it on the agar.
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Capsicum Chinense
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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26791993 - 06/29/20 12:32 PM (3 years, 6 months ago) |
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Quote:
Roger Clemency said: Lol about what though?
MS2G
G2A
A2?
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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As a true freak I suffer from no obias Phrontist 
Agar back to grain (or LC or LI) to finish that thought, only now the myc has been separated from the bacteria that was living all in the syringe water, making it unfeasible to go MSS to agar in the first place.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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jcm4620
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Re: First grow. Spores to agar to grain to monotubs [Re: Roger Clemency]
#26792054 - 06/29/20 01:05 PM (3 years, 6 months ago) |
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heres a good example why syringes suck big floppy donkey balls
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