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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26559394 -

If that’s two weeks old definitely don’t goto grain. Looks iffy to me, Mold perhaps.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26559418 -

Well fuck.  What a waste of time.  I took some better pics.  Ive been fighting a constant nagging mold issue with my monos.  For like months.  So I started from the beginning.  Guess It looks like my source is the problem.




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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26559420 -

maniac1886 said:
Is this ready to transfer to WBS?  This is fifth transfer, 2 weeks(ish) old.
I get that there is bacteria, that just popped up.  Would I transfer from the inner, more mature myc or from the outter reaching ends?  I am going to noc 4 jars.  3 for a mini mono, and 1 as a master.  Should I take 4 wedges?  Any recommendations as to where from?






Edit: seeing them on the computer monitor, looks like they are a bit washed out from the background.  If I need to take better pics, I can definitely do that.



nah mane dont go to grain with a plate that has obvious contam on it. keep trying to clean it up with more transfers. though it doesnt look so good to begin with , so also start fresh just in case

edit: yeah i think you found it


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

Edited by c10h12n2o (03/26/20 05:49 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26559431 -

These yeti plates are looking really promising. Strong thick myc growth.



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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26559453 -

For what it's worth, the agar I'm using may not be the best.  I bought some agar from seaweed laboratories and I found a single thread on this forum that said it was food grade agar and more geared for bacterial growth.  The poster suggested adding potato flakes, which I did, and from then on was able to successfully grow cultures on the agar plates.

So, maybe that is why it takes so long to grow, but this is 5 transfers from what I was suspecting a contaminated LC. I thought I could clean it up, but I guess I don't yet have the eye for it. I guess maybe I'll just scrap it and start from MS  that I have laying around.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26559454 -

They have more than one type of agar. I’ve used their MEA and have had success


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26559469 -

Confirmed.  Since it's looking bad I pulled the lids off of two of them.  They smell like mold. Not myc.    Back to the drawing board!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26559477 -

D3monic said:
These yeti plates are looking really promising. Strong thick myc growth.




what kinda coloring did you use? those plates came out beautiful! what is that tyrian purple lol?

maniac1886 said:
For what it's worth, the agar I'm using may not be the best.  I bought some agar from seaweed laboratories and I found a single thread on this forum that said it was food grade agar and more geared for bacterial growth.  The poster suggested adding potato flakes, which I did, and from then on was able to successfully grow cultures on the agar plates.

So, maybe that is why it takes so long to grow, but this is 5 transfers from what I was suspecting a contaminated LC. I thought I could clean it up, but I guess I don't yet have the eye for it. I guess maybe I'll just scrap it and start from MS  that I have laying around.


are you using their premade MEA mix? im pretty sure that is way higher nutes than people usually use here, like WAY higher, since they use 56g per liter. adding additional nutrients (potato flakes) definitely isnt gonna help. id recommend making your own, its easy af. check my sig. most people here use 15g LME, 20g agar, 1L h2o around here. alternatively, you could probably just use half the amount suggested by seaweed solutions (add more agar agar powder if needed)

proper sterilization will kill any bacteria present, so so the issue is almost certainly your clean technique (based on the satellite colonies of bacteria), and/or dirty spores. are you starting from syringes or prints? SAB or FH? doing any control plates?


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: AGAR ENVY! (Anything and All things agar!) [Re: c10h12n2o] * 1
    #26559506 -

D3monic said:
3 drops magenta in 250ml after boil





c10h12n2o said:
D3monic said:
These yeti plates are looking really promising. Strong thick myc growth.




what kinda coloring did you use? those plates came out beautiful! what is that tyrian purple lol?
maniac1886 said:
I think for that plate it was 2 drops magenta and two
Drops purple


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26559519 -

D3monic said:
These yeti plates are looking really promising. Strong thick myc growth.





Ahhh I was hypnotized by those plates immediately, I haven’t been using food coloring but this inspired me to maybe make some up! I think I saw that brand of food coloring at Vons?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Happycuzimhuntin]
    #26559547 -

20 Days after my very first agar plates, having no clue what I’m looking at, too developing these beauties! These are third transfers! The Fatass I know is a huge piece transferred to the plate but I’m a noob and am trying different things, also the plate it was transferred from was being tossed out anyways:smirk: Thanks for all the useful information from the community! My question is when to transfer these to jars now? And how many one quart jars can a plate be split into?














-Happycuzimgrowin :wink:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: monkey_accident]
    #26560002 -

monkey_accident said:
tedoro said:
roarkell said:
Lately I've been doing 3 bottles of agar at a time so getting the temp just right has been tricky... I poured 75 plates out of three bottles and left them to sit in 6 stacks in the sab in my grow room (ambient temp is around 80f) the first stack I pour I leave in the middle of the sab and then the rest I set around it to kinda insulate it... that first stack had less than half with condensation, all the other stacks were perfect. I was kinda scared of pouring that many all at once but I think it worked well and now I've got a months worth of plates ready whenever.


Why do you suppose the first stack had some condensation, while the latter ones didn't?


Probably because the first stack would be poured hotter than the rest? just a guess


Yeah... the first stack gets poured around 120*F... by the time I'm finishing the third bottle its juuuusssssstttttt starting to set... and I've also noticed that if I just let them sit for a good day or two (which almost never happens...) then they seem to even out completely.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26560039 -

maniac1886 said:
Confirmed.  Since it's looking bad I pulled the lids off of two of them.  They smell like mold. Not myc.    Back to the drawing board!


Annoying I know, what are you using spore wise to get your self going?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smoothcat]
    #26560159 -

Smoothcat said:
maniac1886 said:
Confirmed.  Since it's looking bad I pulled the lids off of two of them.  They smell like mold. Not myc.    Back to the drawing board!


Annoying I know, what are you using spore wise to get your self going?


B+ ms syringe from a reputable source.  It's a year or two old but it's been stored in the zip lock and box it came in, in a drawer in my refrigerator that has nothing else in it.

Edited by maniac1886 (03/27/20 07:53 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886]
    #26560609 -

I don’t think you’re supposed to mention vendors here.

So how are you inoculating your agar then?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: maniac1886] * 1
    #26560614 -

Guys! noob alert

So I stand before for 3rd transfer from the MS I started with and I would really love some professionel advise now, from what to transfer and what not to and more specifically from where int he given plates.

I only have 20 new petri dishes left and I'm having a hard time getting more now because of all the corona stuff going on, so have should I use what I have left?

So here are all 20 plates I have... I spot a couple of plates with some yellow spots (contam I guesss?!?)


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Congame]
    #26560625 -

What’s your plan here?

Honestly none of them look ready for grain yet. You might get away with 7, 17 or 19 but it’s hard to tell from the pics. Do more transfers but don’t use up all your dishes if you can’t get more. Take about 3 transfers to 3 plates from strong clean growth to try and clear them up and work on your sterile tek. Any more would be a waste imo

Good luck


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Congame]
    #26560642 -

Congame said:
Guys! noob alert

So I stand before for 3rd transfer from the MS I started with and I would really love some professionel advise now, from what to transfer and what not to and more specifically from where int he given plates.



My understanding is that there is very little point to isolating MS because you've got no idea if the genetics you're isolating are any good.

Put some of those plates to grain, fruit, look for a strong cluster, take a tissue sample *then* start playing with agar.

The only point of agar with MS is to cut out contams which you've certainly done at this point.


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
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Re: AGAR ENVY! (Anything and All things agar!) [Re: metaphoric]
    #26560652 -

metaphoric said:
Congame said:
Guys! noob alert

So I stand before for 3rd transfer from the MS I started with and I would really love some professionel advise now, from what to transfer and what not to and more specifically from where int he given plates.



My understanding is that there is very little point to isolating MS because you've got no idea if the genetics you're isolating are any good.

Put some of those plates to grain, fruit, look for a strong cluster, take a tissue sample *then* start playing with agar.

The only point of agar with MS is to cut out contams which you've certainly done at this point.

 

Take a tissue sample like this...RR picks a clone  be sure to label the shroom and plate...eat the shroom!

Edited by Inthepit (03/27/20 09:38 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Congame]
    #26560675 -

Congame said:
Guys! noob alert

So I stand before for 3rd transfer from the MS I started with and I would really love some professionel advise now, from what to transfer and what not to and more specifically from where int he given plates.

I only have 20 new petri dishes left and I'm having a hard time getting more now because of all the corona stuff going on, so have should I use what I have left?

So here are all 20 plates I have... I spot a couple of plates with some yellow spots (contam I guesss?!?)




I used to be like this and have loads of plates.  No I work like this.  For every culture I have I am looking to do 2 things.  I am trying to  A- get a clean culture to use for grains, LC's LI's ect and B - 1 to store.

With this in mind from every clone plate(I could take 3 clones at 1 time), spore plates (usually 3)  I take 2 transfers per culture.  Once I get a clean culture on 1 or both new plate, 1 goes to grains or LC and 1 goes in storage.  With spore plates im trying to grow so I can get clones. With clone plates If that culture is really good I G2G as long as I can till finally it gets dirty or i get bored and then go back to the storage plate and make new plates- One goes to grains/LC and the other storage, repeat.  If only one is clean I keep transferring from the non 100% plate till I am happy with it, then store.

In the beginning, like you,  I used every vessel in my house to grow cultures and it got overwhelming.  I using maybe 30 plates at the mo but have 12-15 cultures im experimenting with.

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