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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: Myyco.com Golden Teacher Liquid Culture For Sale, Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck, Certified Organic All-In-One Grow Bags by Magic Bag

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Re: Making Liquid Culture [Re: mushboy]
    #26452255 -

mushboy said:
A.k.a said:
I’m also wondering about that.



I take lc out while hot, let it cool then tighten. Leaving the foil on the whole time. I never leave things to cool in the pc.

No vacuum:awedance:



I did this, there was still a whooosh. Here's hoping it was positive pressure escaping. We shall see, and i will endeavor to do it this way.


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Re: Making Liquid Culture [Re: Kimble]
    #26452261 -

I've been all paranoid lately, so I've been moving the PC in front of
the hood right as it reaches 0 psi on the gauge but still has
pressure. I'll leave it there for an hour or so and then open it
up and make sure all the lids are tight.

mushboy, is this something a crazy person does? :lol:


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Cultivating Panaeolus cyanescens


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Re: Making Liquid Culture [Re: Asura] * 1
    #26452274 -

First LC done. I didn't notice any vacuum when opening. 


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FIRST GROW getting started
https://www.shroomery.org/forums/postlist.php/Journal/484135

Joobys 2020 LAGM growlog

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Re: Making Liquid Culture [Re: Asura]
    #26452290 -

Asura said:
mushboy, is this something a crazy person does? :lol:


Crazy person with a fh. If I had one I'd do that as well.

Someday:rolleyes:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26452426 -

I’ve been wanting one to but my space is just too small. :crying:


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Tek's I use

LAGM2020

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Re: Making Liquid Culture [Re: staytrippy420] * 1
    #26460407 -

5 days later. Mid swirl. I'm in love.


Fir the sake of experiment I also noc'd a ball jar. Mushboy, is this no bueno?


I've used red food coloring but dont recall if this has it, I was drunk. If it's no good, I might drink it...looks like goldschlager


--------------------
FIRST GROW getting started
https://www.shroomery.org/forums/postlist.php/Journal/484135

Joobys 2020 LAGM growlog

Edited by Jooby (01/30/20 02:49 PM)

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Re: Making Liquid Culture [Re: Jooby]
    #26460759 -



Day six.


I also picked these up:


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Re: Making Liquid Culture [Re: Kimble] * 1
    #26460773 -

Kimble said:



That magnetic bar with the bump on the middle will make a lot of noise on the stir plate. I stopped using them cause it was driving me crazy:D


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====> Making a timelapse video with a Raspberry Pi and a webcam <====

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Re: Making Liquid Culture [Re: eLeSDenes] * 1
    #26462887 -

eLeSDenes said:
Kimble said:



That magnetic bar with the bump on the middle will make a lot of noise on the stir plate. I stopped using them cause it was driving me crazy:D


A citrus rasp removes them well.


--------------------
Question everything, it's very responsive.
culture storage

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Re: Making Liquid Culture [Re: Nichrome] * 1
    #26463222 -

You really only need to whip an LC with a stir bar once or twice a day for less than a minute so I don't know why you would complain about the noise it's not that loud. There's not much difference running it 24/7 and many stir plates will overheat your lc if you do that anyway because the motors can get warm especially when dialed down low because of the rheostat. If you do want to run your LC stirring for 24/7 you better test your stir plate by letting it run for a few days and feel if the base plate is warm where the LC sits. I once ruined a whole bunch of LC after finding out my old 4 position stirrer gets warm when run 24/7.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Edited by sandman420 (02/01/20 08:40 AM)

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Re: Making Liquid Culture [Re: sandman420] * 1
    #26463332 -

Seems to be species and/or culture specific as well. Pan cyans respond extremely
well to the 24/7 stirring. I can have an LC ready in 4 days.

Zapotecorum absolutely does not like constant agitation. Same with semperviva and
caerulescens. Some cubes do but others do not :shrug:

Fast growing vs. slow? I'm not sure.


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Cultivating Panaeolus cyanescens


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Re: Making Liquid Culture [Re: Asura] * 1
    #26463344 -

Once I did PE with 24/7 stirring and a star shaped bar and it grew these little spiked balls of mycelium that looked like naval mines. It was wild and I regret not getting any pics.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Making Liquid Culture [Re: sandman420] * 1
    #26463394 -

sandman420 said:
Once I did PE with 24/7 stirring and a star shaped bar and it grew these little spiked balls of mycelium that looked like naval mines. It was wild and I regret not getting any pics.


Do you mean just like my first Estero culture? This is today.

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Re: Making Liquid Culture [Re: Puduwoke]
    #26463446 -

oh snit wow yep just like that.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Making Liquid Culture [Re: sandman420]
    #26463494 -



My rustywhyte is doing the same
I keep it on a low swirl and crank it once a day or so to really blend
Think im gonna shut off for the weekend and let it fluff up


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.
Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can

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Re: Making Liquid Culture [Re: Camera93]
    #26465071 -

I’m guessing this one on the left is fucked??



I’ve made seven and this is the only cloudy one.



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LAGM2020

Edited by A.k.a (02/02/20 10:53 AM)

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Re: Making Liquid Culture [Re: A.k.a]
    #26465136 -

yep. one i contamed on purpose for an example:




this one smelled like cheese!

your other ones look solid though :rockon:


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Re: Making Liquid Culture [Re: A.k.a]
    #26465139 -

I'd say so.

Big reason why I like clear broths. It goes cloudy? Its fucked:sad:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Making Liquid Culture [Re: mushboy]
    #26465775 -

mushboy said:
I'd say so.

Big reason why I like clear broths. It goes cloudy? Its fucked:sad:


My looked cloudy but it was clean, i did it with .8% nutes tho. Next time i'll do it with .4%


--------------------

My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Making Liquid Culture [Re: Mr. Alien]
    #26466553 -

I have a few questions with regards to water content and LC in general, thanks for the help!

Say I put 50 CCs of LC into a jar of oats, if the oats were properly sterilized, slightly under hydrated and my LC was 100% clean, what’s the downside if the extra LC is absorbed by the oats (even if it takes a shake or two)?

Will it get contaminated if there aren’t any other competing organisms? I’ve been putting ships on my LC bottles and use micropore for GE. Am I overestimating the effectiveness of PC sterilization and multiple layers of micropore and SHIPS?

Last question, when is a shake recommended when using LC (if any)?


--------------------
Looking to learn all about it

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale, Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck, Certified Organic All-In-One Grow Bags by Magic Bag

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